Skip to main content
SpringerLink
Log in

Impact of moisture, host genetics and Fusarium graminearum isolates on Fusarium head blight development and trichothecene accumulation in spring wheat

  • Original Paper
  • Published:
Mycotoxin Research Aims and scope Submit manuscript

Abstract

The impact of moisture on the development of Fusarium head blight (FHB) and accumulation of deoxynivalenol (DON) in Fusarium-infected wheat was examined. The field experiments were designed as split-split-plot with five replicates. Main plots were durations of mist-irrigation [14, 21, 28 and 35 days after inoculation (DAI)]; sub-plots were wheat cultivar; and sub-sub-plots were F. graminearum isolates differing in aggressiveness and DON production capacity. The wheat cultivars ‘Alsen’ (moderately resistant), ‘2375’ (moderately susceptible) and ‘Wheaton’ (susceptible) were inoculated at anthesis. Severity of FHB was assessed 21 days after inoculation. Visually scabby kernels (VSK) and mycotxin content (DON, 15-AcDON, 3-AcDON and nivalenol) were determined on harvested grain. The damage to grain, as measured by VSK, was significantly lower in the treatments receiving the least amount of mist-irrigation (14 DAI) suggesting that extended moisture promotes disease development. DON was, however, significantly lower in the 35-DAI misting treatment than in treatments receiving less post-inoculation moisture. The reduction of DON observed in treatments receiving extended mist-irrigation was greatest in ‘Wheaton’ which recorded the highest FHB severity, VSK and DON of the cultivars examined. Our results suggest that DON and other trichothecenes may be reduced by late-season moisture despite increased grain colonization. We suggest that leaching may explain much of the reduction of mycotoxins, and that differences in tissue morphology and metabolism may determine the rate of leaching from specific tissues.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

References

  • Abramson D, Clear RM, Nowicki TW (1987) Fusarium species and trichothecene mycotoxins in suspect samples of 1985 manitoba wheat. Can J Plant Sci 67:611–619

    Article  CAS  Google Scholar 

  • Akinsanmi OA, Backhouse D, Simpfendorfer S, Chakraborty S (2006) Pathogenic variation of Fusarium isolates associated with head blight of wheat in Australia. J Phytopathol 154:513–521

    Article  Google Scholar 

  • Argyris J, Van Sanford D, TeKrony D (2003) Fusarium graminearum infection during wheat seed development and its effect on seed quality. Crop Sci 43:1782–1788

    Article  Google Scholar 

  • Atanasoff D (1920) Fusarium-blight (scab) of wheat and other cereals. J Agric Res 20:1–40

    Google Scholar 

  • Bai GH, Shaner G (1996) Variation in Fusarium graminearum and cultivar resistance to wheat scab. Plant Dis 80:975–979

    Article  Google Scholar 

  • Bai GH, Shaner G, Desjardin AE, Kolb FL (1994) Scab of wheat: Prospects for control. Plant Dis 78:760–766

    Article  Google Scholar 

  • Bai GH, Plattner R, Desjardin A, Kolb F (2001) Resistance to Fusarium head blight and deoxynivalenol accumulation in wheat. Plant Breed 120:1–6

    Article  CAS  Google Scholar 

  • Berthiller F, Lemmens M, Werner U, Krska R, Hauser M, Adam G, Schuhmacher R (2007) Short review: Metabolism of the Fusarium mycotoxins deoxynivalenol and zearalenone in plants. Mycotoxin Res 23:68–72

    Article  CAS  Google Scholar 

  • Böhm C, Cichna-Markl M, Brenn-Struckhofova Z, Razzazi-Fazeli E (2008) Development of a selective sample clean-up method based on immuno-ultrafiltration for the determination of deoxynivalenol in maize. J ChromA 1202:111–117

    Article  Google Scholar 

  • Cahill LM, Kruger SC, McAlice BT, Ramsey CS, Prioli R, Kohn B (1999) Quantification of deoxynivalenol in wheat using an immunoaffinity column and liquid chromatography. J ChromA 859:23–28

    Article  CAS  Google Scholar 

  • Carter JP, Rezanoor HN, Holden D, Desjardins AE, Plattner RD, Nicholson P (2002) Variation in Pathogenicity Associated with the Genetic Diversity of Fusarium graminearum. Eur J Plant Pathol 108:573–583

    Article  CAS  Google Scholar 

  • Cowger C, Patton-Özkurt J, Brown-Guedira G, Perugini L (2009) Post-anthesis moisture increased Fusarium head blight and deoxynivalenol levels in North Carolina winter wheat. Phytopathology 99:320–327

    Article  PubMed  CAS  Google Scholar 

  • Culler MD, Miller-Garvin JE, Dill-Macky R (2007) Effect of extended irrigation and host resistance on deoxynivalenol accumulation in Fusarium-infected wheat. Plant Dis 91:1464–1472

    Article  CAS  Google Scholar 

  • De Wolf ED, Madden LV, Lipps PE (2003) Risk assessment models for wheat Fusarium head blight epidemics based on within-season weather data. Phytopathology 93:428–435

    Article  PubMed  Google Scholar 

  • Dill-Macky R (2003) Inoculation methods and evaluation of Fusarium head blight resistance in wheat. In: Leonard KJ, Bushnell WR (eds) Fusarium Head Blight of Wheat and Barley. The American Phytopathological Society, St. Paul, MN, pp 184–210

    Google Scholar 

  • Evans CK, Xie W, Dill-Macky R, Mirocha CJ (2000) Biosynthesis of Deoxynivalenol in Spikelets of Barley Inoculated with Macroconidia of Fusarium graminearum. Plant Dis 84:654–654

    Article  CAS  Google Scholar 

  • Fuentes RG, Mickelson HR, Busch RH, Dill-Macky R, Evans CK, Thompson WG, Wiersma JV, Xie W, Dong Y, Anderson JA (2005) Resource allocation and cultivar stability in breeding for Fusarium head blight resistance in spring wheat. Crop Sci 45:1965–1972

    Article  CAS  Google Scholar 

  • Gang G, Miedaner T, Schuhmacher U, Schollenberger M, Geiger HH (1998) Deoxynivalenol and nivalenol production by Fusarium culmorum isolates differing in aggressiveness toward winter rye. Phytopathology 88:879–884

    Article  PubMed  CAS  Google Scholar 

  • Garda-Buffon J, Badiale-Furlong E (2010) Kinetics of deoxynivalenol degradation by Aspergillus oryzae and Rhizopus oryzae in submerged fermentation. J Braz Chem Soc 21:710–714

    Article  CAS  Google Scholar 

  • Gautam P, Dill-Macky R (2012) Fusarium head blight development and trichothecene accumulation in point inoculated Fusarium-infected wheat heads. World Mycotoxin J (Accepted for publication; doi:10.3920/WMJ2011.1315)

  • Goswami RS, Kistler HC (2005) Pathogenicity and in planta mycotoxin accumulation among members of the Fusarium graminearum species complex on wheat and rice. Phytopathology 95:1397–1404

    Article  PubMed  CAS  Google Scholar 

  • Groth JV, Ozmon EA, Busch RH (1999) Repeatability and relationship of incidence and severity measures of scab of wheat caused by Fusarium graminearum in inoculated nurseries. Plant Dis 83:1033–1038

    Article  Google Scholar 

  • Hooker DC, Schaafsma AW, Tamburic-Ilincic L (2002a) Using weather variables pre- and post-heading to predict deoxynivalenol content in winter wheat. Plant Dis 86:611–619

    Article  Google Scholar 

  • Hooker DC, Shaafsma AW, Tamburic-Ilincic L (2002b) The DONcast model: using weather variables pre- and postheading to predict deoxynivalenol content in winter wheat. In: Canty SM, Lewis J, Siler L, Ward RW (eds) Proceedings of 2002 National Fusarium Head Blight Forum. Michigan State University, MI, Erlanger, KY, p 165

    Google Scholar 

  • Hope R, Aldred D, Magan N (2005) Comparison of environmental profiles for growth and deoxynivalenol production by Fusarium culmorum and F. graminearum on wheat grain. Lett Appl Microbiol 40:295–300

    Article  PubMed  CAS  Google Scholar 

  • Jones RK, Mirocha CJ (1999) Quality parameters in small grains from Minnesota affected by Fusarium head blight. Plant Dis 83:506–511

    Article  Google Scholar 

  • Kang Z, Buchenauer H (1999) Immunocytochemical localization of Fusarium toxins in infected wheat spikes by Fusarium culmorum. Physiol Mol Plant Pathol 55:275–288

    Article  CAS  Google Scholar 

  • Kang Z, Buchenauer H (2000) Ultrastructural and immunocytochemical investigation of pathogen development and host responses in resistance and susceptible wheat spikes infected by Fusarium culmorum. Physiol Mol Plant Pathol 57:255–268

    Article  CAS  Google Scholar 

  • Kollarczik B, Gareis M, Hanelt M (1994) In vitro transformation of the Fusarium mycotoxins deoxynivalenol and zearalenone by the normal gut microflora of pigs. Nat Toxins 2:105–110

    Article  PubMed  CAS  Google Scholar 

  • Lacey J, Bateman GL, Mirocha CJ (1999) Effects of infection time and moisture on development of ear blight and deoxynivalenol production by Fusarium spp. in wheat. Ann Appl Biol 134:277–283

    Article  CAS  Google Scholar 

  • Lemmens M, Buerstmayr H, Krska R, Schuhmacher R, Grausgruber H, Ruckenbauer P (2004) The effect of inoculation treatment and long-term application of moisture on Fusarium head blight symptoms and deoxynivalenol contamination in wheat grains. Eur J Plant Pathol 110:299–308

    Article  CAS  Google Scholar 

  • Lemmens M, Scholz U, Berthiller F, Dall’Asta C, Koutnik A, Schuhmacher R, Adam G, Buerstmayr H, Mesterházy Ã, Krska R, Ruckenbauer P (2005) The ability to detoxify the mycotoxin deoxynivalenol colocalizes with a major quantitative trait locus for Fusarium head blight resistance in wheat. Mol Plant Microbe Interact 18:1318–1324

    Article  PubMed  CAS  Google Scholar 

  • McMullen M, Jones R, Gallenberg D (1997) Scab of Wheat and Barley: A Re-emerging Disease of Devastating Impact. Plant Dis 81:1340–1348

    Article  Google Scholar 

  • Mesterházy Á, Bartók T, Lamper C (2003) Influence of wheat cultivar, species of Fusarium, and isolate aggressiveness on the efficacy of fungicides for control of Fusarium head blight. Plant Dis 87:1107–1115

    Article  Google Scholar 

  • Miller JD, Arnison PG (1986) Degredatioin of deoxynivalenol by suspension cultures of the Fusarium head blight resistant wheat cultivar Frontana. Can J Plant Pathol 8:147–150

    Article  CAS  Google Scholar 

  • Miller JD, Young JC (1985) Deoxynivalenol in an experimental Fusarium graminearum infection of wheat. Can J Plant Pathol 7:132–134

    Article  CAS  Google Scholar 

  • Miller JD, Young JC, Sampson DR (1985) Deoxynivalenol and Fusarium head blight resistance in spring cereals. Phytopathol Z 113:359–367

    Article  CAS  Google Scholar 

  • Mirocha CJ, Kolaczkowski E, Xie W, Yu H, Jelen H (1998) Analysis of Deoxynivalenol and Its Derivatives (Batch and Single Kernel) Using Gas Chromatography/Mass Spectrometry. J Agric Food Chem 46:1414–1418

    Article  CAS  Google Scholar 

  • Nasri T, Bosch RR, Voorde ST, Fink-Gremmels J (2006) Differential induction of apoptosis by type A and B trichothecene in Jurkat T-lymphocytes. Toxicol In Vitro 20:832–840

    Article  PubMed  CAS  Google Scholar 

  • Nganje WE, Bangsund DA, LLeistritz FL, Wilson WW, Tiapo NM (2002) Estimating the economic impact of a crop disease: the case of Fusarium head blight in US wheat and barley. In: Canty SM, Lewis J, Siler L, Ward RW (eds) Proceedings of 2002 National Fusarium Head Blight Forum. Michigan State University, MI, Erlanger, KY, pp 275–281

    Google Scholar 

  • Pirgozliev SR, Edwards SG, Hare MC, Jenkinson P (2003) Strategies for the control of Fusarium head blight in cereals. Eur J Plant Pathol 109:731–742

    Article  Google Scholar 

  • Prandini A, Sigolo S, Filippi L, Battilani P, Piva G (2009) Review of predictive models for Fusarium head blight and related mycotoxin contamination in wheat. Food Chem Toxicol 47:927–931

    Article  PubMed  CAS  Google Scholar 

  • Rohácik T, Hudec K (2005) Influence of agro-environmental factors on Fusarium infestation and population structure in wheat kernels. Ann Agric Environ Med 12:39–45

    PubMed  Google Scholar 

  • Saxton AM (1998) A macro for converting mean separation output to letter groupings in Proc Mixed. In: Proceedings of 23rd SAS Users Group International SAS Institute, Cary, NC, pp 1243–1246

  • Schroeder HW, Christensen JJ (1963) Factors affecting resistance of wheat to scab caused by Gibberella zeae. Phytopathology 53:831–838

    Google Scholar 

  • Scott PM, Nelson K, Kanhere SR, Karpinski KF, Hayward S, Neish GA, Teich AH (1984) Decline in deoxynivalenol (vomitoxin) concentrations in 1983 Ontario winter wheat before harvest. App Environ Microbiol 48:884–886

    CAS  Google Scholar 

  • Snijders CHA, Krechting CF (1992) Inhibition of deoxynivalenol translocation and fungal colonization in Fusarium head blight resistant wheat. Can J Bot 70:1570–1576

    Article  CAS  Google Scholar 

  • Tóth B, Mesterházy Á, Horváth Z, Bartók T, Varga M, Varga J (2005) Genetic Variability of Central European Isolates of the Fusarium graminearum Species Complex. Eur J Plant Pathol 113:35–45

    Article  Google Scholar 

  • Tu C (1929) Physiological specialization in Fusarium spp. causing head blight of small grains. Phytopathology 53:831–838

    Google Scholar 

  • Tu C (1930) Physiologic specialization in Fusarium spp. causing headblight of small grains. In: Minnesota Agricultural Experiment Station Technical Bulletin

  • Tuite J, Shaner G, Everson RJ (1990) Wheat Scab in Soft Red Winter Wheat in Indiana in 1986 and Its Relation to Some Quality Measurements. Plant Dis 74:959–962

    Article  Google Scholar 

  • Walker SL, Leath S, Hagler WM, Murphy JP (2001) Variation among isolates of Fusarium graminearum associated with Fusarium head blight in North Carolina. Plant Dis 85:404–410

    Article  Google Scholar 

  • Wilde F, Miedaner T (2006) Selection for Fusarium head blight resistance in early generations reduces the deoxynivalenol (DON) content in grain of winter and spring wheat. Plant Breed 125:96–98

    Article  CAS  Google Scholar 

  • Xue AG, Ho KM, Babcock C, Chen Y, Sabo F, Kuc M(2004). Pathogenicity of Fusarium species causing headblight in barley. Can J Plant Pathol 26:429 (abstract)

    Google Scholar 

  • Zadoks JC, Chang TT, Konzak CF (1974) A decimal code for the growth stages of cereals. Weed Res 14:415–421

    Article  Google Scholar 

Download references

Acknowledgements

We would like to thank Beheshteh Zagaran, Amar M. Elakkad, and Karen J. Wennberg, for their technical assistance and Dr. Yanhong Dong for mycotoxin analyses. This material is based upon work supported by the U.S. Department of Agriculture, under agreement No. 59-0790-4-096. This is a cooperative project with the U.S. Wheat and Barley Scab Initiative.

Conflict of interest

None

Author information

Author notes

  1. Pravin Gautam

    Present address: Department of Plant Science, South Dakota State University, Brookings, SD, 57007, USA

Authors and Affiliations

  1. Department of Plant Pathology, University of Minnesota, St. Paul, MN, 55108, USA

    Pravin Gautam & Ruth Dill-Macky

Authors
  1. Pravin Gautam
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Ruth Dill-Macky
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Ruth Dill-Macky.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Gautam, P., Dill-Macky, R. Impact of moisture, host genetics and Fusarium graminearum isolates on Fusarium head blight development and trichothecene accumulation in spring wheat. Mycotoxin Res 28, 45–58 (2012). https://doi.org/10.1007/s12550-011-0115-6

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12550-011-0115-6

Keywords

Search

Navigation