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In vitro fertilization embryo development from caffeine-treated murine sperm

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Reproductive Medicine and Biology

Abstract

Purpose

To evaluate the effect of long-term caffeine administration on murine sperm and subsequent in vitro fertilization (IVF).

Methods

Male mice were injected with various doses (0, 0.2 and 1.0 mg/mouse/day) of caffeine for 1 month. After sperm collection, the IVF rate and embryo development to the blastocyst stage were evaluated.

Results

The mean body weight significantly decreased in the 1.0 mg/day treatment group compared to the control group (P < 0.01). Testicular weight and histological features did not differ, and total blood testosterone was no different in spite of the difference between 0.2 and 1.0 mg/day of caffeine. The IVF rate differed significantly between the control group [100/105 (95.2 %)] and 0.2 mg/day group [106/121 (87.6 %)] (P < 0.05). Furthermore, blastocyst formation was significantly and dose-dependently lower with higher caffeine levels: control group: 85/100 (85.0 %); 0.2 mg/day group: 84/106 (79.2 %) (P < 0.05); 1.0 mg/day group: 64/102 (62.7 %) (P < 0.001).

Conclusions

Caffeine treatment affected body weight of male mice. However, testicular weight, histological features and total blood testosterone concentration were not statistically different. In addition, following IVF using sperm from these mice, blastocyst formation decreased in a dose-dependent manner. These findings suggest that embryo development from oocytes fertilized with sperm from caffeine-administered male mice is negatively affected.

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Conflict of interest

Yoshimasa Yokota, Hidemi Yokota, Mikako Yokota, Yasuyuki Araki and Yasuhisa Araki declare that they have no conflict of interest.

Human rights

This article does not contain any studies with human subjects performed by any of the authors.

Animal studies

All institutional and national guidelines for the care and use of laboratory animals were followed.

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Authors and Affiliations

  1. Yokota Maternity Hospital, 1-5-22 Shimokoide, Maebashi, Gunma, 371-0031, Japan

    Yoshimasa Yokota, Hidemi Yokota & Mikako Yokota

  2. The Institute for Advanced Reproductive Medical Technology, 909-21 Ishii, Fujimi, Maebashi, Gunma, 371-0105, Japan

    Yasuyuki Araki & Yasuhisa Araki

Authors
  1. Yoshimasa Yokota
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  2. Hidemi Yokota
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  3. Mikako Yokota
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  4. Yasuyuki Araki
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  5. Yasuhisa Araki
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Corresponding author

Correspondence to Yoshimasa Yokota.

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Yokota, Y., Yokota, H., Yokota, M. et al. In vitro fertilization embryo development from caffeine-treated murine sperm. Reprod Med Biol 14, 117–121 (2015). https://doi.org/10.1007/s12522-015-0203-y

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  • DOI: https://doi.org/10.1007/s12522-015-0203-y

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