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Effect of long-term caffeine administration to mice on in vitro fertilization and embryo development using oocytes

  • Original Article
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Reproductive Medicine and Biology

Abstract

Purpose

To evaluate the effect of long-term caffeine administration to mice on in vitro fertilization (IVF) of oocytes.

Methods

Mice were injected with different dosages (0, 0.1, and 1.0 mg/mouse/converted day) of caffeine for one month. Subsequently, the fertilization rate and embryo development to blastocyst stage were evaluated in IVF using oocytes from the mice.

Results

The retrieved average oocyte rate was significantly lower (27.4) in mice injected with 1.0 mg caffeine than in the control group (36.5; P < 0.05); the fertilization rate was significantly different between the 0 mg (317/401; 79.1 %) and 1.0 mg group (199/301; 66.1 %) (P < 0.05). At 96 h after insemination, the blastocyst formation rate was significantly decreased in the 1.0 mg group (94/199; 47.2 %) compared with the control (0 mg) group (237/317; 74.8 %) and 0.1 mg group (226/323; 70 %) (P < 0.05). When 1.0 mg caffeine was administered for two weeks, embryo development was significantly impacted.

Conclusions

Our findings suggest that caffeine administration negatively impacts oocytogenesis and embryonic development after IVF.

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Conflict of interest

We have no conflict of interest.

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Authors and Affiliations

  1. Yokota Maternity Hospital, 1-5-22 Shimokoide, Maebashi, Gunma, 371-0031, Japan

    Hidemi Yokota, Yoshimasa Yokota & Mikako Yokota

  2. The Institute for Advanced Reproductive Medical Technology, 909-21 Ishii, Fujimi, Maebashi, Gunma, 371-0105, Japan

    Yasuyuki Araki & Yasuhisa Araki

Authors
  1. Hidemi Yokota
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  2. Yoshimasa Yokota
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  3. Mikako Yokota
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  4. Yasuyuki Araki
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  5. Yasuhisa Araki
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Corresponding author

Correspondence to Yoshimasa Yokota.

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Yokota, H., Yokota, Y., Yokota, M. et al. Effect of long-term caffeine administration to mice on in vitro fertilization and embryo development using oocytes. Reprod Med Biol 12, 167–171 (2013). https://doi.org/10.1007/s12522-013-0150-4

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  • DOI: https://doi.org/10.1007/s12522-013-0150-4

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