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Combined osteomorphological, isotopic, aDNA, and ZooMS analyses of sheep and goat remains from Neolithic Ulucak, Turkey

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Abstract

The site of Ulucak is pivotal in exploring the Neolithic of the eastern Aegean and western Anatolia. It has an impressive stratigraphic sequence stretching from the first half of the seventh millennium BC to the first half of the sixth millennium BC. Recent zooarchaeological analyses have provided insight into the importance of animal husbandry practices and dairying at the site throughout the Neolithic but also raised questions about the changing nature of herd management strategies and whether these differed for sheep and goat. The faunal data, coupled with the significance of the site and condition of the assemblage, prompted the application of a number of methodological techniques to assess differences between sheep and goat. In this paper, we combine traditional osteomorphological analysis, ancient DNA, collagen peptide mass fingerprinting (zooarchaeology by mass spectrometry, or ZooMS), and stable isotope analysis of δ18O and δ13C from tooth enamel carbonate as well as δ13C and δ15N from bone collagen. As such, this is the first study of its kind. We evaluate the juxtaposition of these four approaches and their application in this important case, with relevance for future studies in the region.

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References

  • Ambrose SH (1990) Preparation and characterization of bone and tooth collagen for isotopic analysis. J Archaeol Sci 17(4):431–451

    Article  Google Scholar 

  • Ambrose SH (1991) Effects of diet, climate and physiology on nitrogen isotope abundances in terrestrial foodwebs. J Archaeol Sci 18(3):293–317

    Article  Google Scholar 

  • Arbuckle BS, Atici L (2013) Initial diversity in sheep and goat management in Neolithic south-western Asia. Levant 45(2):219–235

    Article  Google Scholar 

  • Arbuckle BS, Kansa SW, Kansa E, Orton D, Çakirlar C, Gourichon L, Atici L et al (2014) Data sharing reveals complexity in the westward spread of domestic animals across Neolithic Turkey. PLoS One 9(6):e99845

    Article  Google Scholar 

  • Atici L, Kansa SW, Lev-Tov J, Kansa EC (2013) Other people’s data: a demonstration of the imperative of publishing primary data. J Archaeol Method Theory 20:663–681

    Article  Google Scholar 

  • Atici L, Pilaar Birch SE, Erdoğu B (2017) Spread of domestic animals across Neolithic western Anatolia: new zooarchaeological evidence from Uğurlu Höyük, the island of Gökçeada, Turkey. PLoS One 12(10):e0186519

    Article  Google Scholar 

  • Balasse M (2002) Reconstructing dietary and environmental history from enamel isotopic analysis: time resolution of intra-tooth sequential sampling. Int J Osteoarchaeol 12:155–165

    Article  Google Scholar 

  • Balasse M (2013) Seasonality and season of birth in early Eneolithic sheep from Cheia (Romania): methodological advances and implications for animal economy. J Archaeol Sci 40:4039–4055

    Article  Google Scholar 

  • Balasse M, Ambrose SH, Smith A, Price TD (2002) The seasonal mobility model for prehistoric herders in the south-western cape of South Africa assessed by isotopic analysis of sheep tooth enamel. J Archaeol Sci 29:917–932

    Article  Google Scholar 

  • Balasse M, Smith AB, Ambrose SH, Leigh SR (2003) Determining sheep birth seasonality by analysis of tooth enamel oxygen isotope ratios: the Late Stone Age site of Kasteelberg (South Africa). J Archaeol Sci 30:205–215

    Article  Google Scholar 

  • Balasse M, Obein G, Ughetto-Monfrin J, Mainland I (2012) Investigating seasonality and season of birth in past herds: a reference set of sheep enamel stable oxygen isotope ratios. Archaeometry 54:349–368

    Article  Google Scholar 

  • Bar-Gal GK, Ducos P, Horwitz LK (2003) The application of ancient DNA analysis to identify neolithic caprinae: a case study from the site of Hatoula, Israel. Int J Osteoarchaeol 13(3):120–131

    Article  Google Scholar 

  • Boessneck J (1970) Osteological differences between sheep (Ovis ariea Linne) and goats (Capra hircus Linne). In: Brothwell D, Higgs E (eds) Science in archaeology. Praeger, New York, pp 331–358

    Google Scholar 

  • Boessneck J, Müller H -H, Teichert M (1964) Osteologische Unterscheidungmerkmale zwischen Schaf (Ovis aries Linne) und Zeige (Capra hircus Linne). Kuhn-Archiv 78:1–29

  • Bollongino R, Vigne J-D (2008) Temperature monitoring in archaeological animal bone samples in the Near East arid area, before, during and after excavation. J Archaeol Sci 35:873–881

    Article  Google Scholar 

  • Bollongino R, Tresset A, Vigne J-D (2008) Environment and excavation: pre-lab impacts on ancient DNA analyses. Comptes Rendus Palevol 7:91–98

    Article  Google Scholar 

  • Brami MN (2014) A graphical simulation of the 2,000-year lag in Neolithic occupation between Central Anatolia and the Aegean basin. Archaeol Anthropol Sci, 1–9

  • Brown S, Higham T, Slon V, Paabo S, Meyer M, Douka K, Brock F, Comesky D, Procopio N, Shunkov M, Derevianko A, Buckley M (2016) Identification of a new hominin bone from Denisova Cave, Siberia using collagen fingerprinting and mitochondrial DNA analysis. Sci Rep 6:23559

    Article  Google Scholar 

  • Buckley M, Collins MJ (2011) Collagen survival and its use for species identification in Holocene-lower Pleistocene bone fragments from British archaeological and palaeontological sites. Antiqua 1(1):e1

    Article  Google Scholar 

  • Buckley M, Kansa SW (2011) Collagen fingerprinting of archaeological bone and teeth remains from Domuztepe, South Eastern Turkey. Archaeol Anthropol Sci 3(3):271–280

    Article  Google Scholar 

  • Buckley M, Collins M, Thomas-Oates J (2008) A method of isolating the collagen (I) α2 chain carboxytelopeptide for species identification in bone fragments. Anal Biochem 374(2):325–334

    Article  Google Scholar 

  • Buckley M, Collins M, Thomas-Oates J, Wilson JC (2009) Species identification by analysis of bone collagen using matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry. Rapid Commun Mass Spectrom: RCM 23(23):3843–3854

    Article  Google Scholar 

  • Buckley M, Whitcher Kansa S, Howard S, Campbell S, Thomas-Oates J, Collins M (2010) Distinguishing between archaeological sheep and goat bones using a single collagen peptide. J Archaeol Sci 37(1):13–20

    Article  Google Scholar 

  • Buckley M, Fraser S, Herman JS, Melton ND, Mulville J, Pálsdóttir AH (2014) Species identification of archaeological marine mammals using collagen fingerprinting. J Archaeol Sci 41:631–641

    Article  Google Scholar 

  • Buckley M, Gu M, Shameer S, Patel S, Chamberlain AT (2016) High-throughput collagen fingerprinting of intact microfaunal remains; a low-cost method for distinguishing between murine rodent bones. Rapid Commun Mass Spectrom 30:1–8

    Article  Google Scholar 

  • Çakırlar C (2012a) The evolution of animal husbandry in Neolithic central-west Anatolia: the zooarchaeological record from Ulucak Höyük (c. 7040–5660 cal. BC, Izmir, Turkey). Anatol Stud 62:1–33

    Article  Google Scholar 

  • Çakırlar C (2012b) Neolithic dairy technology at the European–Anatolian frontier: implications of archaeozoological evidence from Ulucak Höyük, Turkey, ca. 7000–5700 cal BC. Anthropozoologica 47(2):77–98

    Article  Google Scholar 

  • Çakırlar C (2015) Adaptation, identity, and innovation in Neolithic and Chalcolithic Western Anatolia (6800–3000 cal. BC): the evidence from aquatic mollusk shells. Quat Int 390:117–125

    Article  Google Scholar 

  • Çakirlar C (2016) Early bronze age foodways in the Aegean: social archaeozoology on the eastern side. In Pernicka E, ÜnlÜsoy S, Blum S (Eds). Early bronze age troy: chronology, cultural development and interregional contacts: proceedings of an international conference held at the University of TÜbingen, May 8–10, 2009. (pp. 291–303). Verlag Rudolf Habert

  • Çakırlar C, Atici L (2017) Patterns of animal exploitation in western Turkey from Palaeolithic molluscs to Byzantine elephants. In: Albarella U, Russ H, Vickers K, Viner-Daniels S (eds) Oxford handbook of zooarchaeology. Oxford University Press, Oxford, pp 266–279

    Google Scholar 

  • Çevik Ö (2013) Ulucak Höyük 2009-2011 yılı kazı çalışmaları. Kazı Sonuçları Toplantısı 34:143–158

  • Çilingiroğlu Ç (2017) The Aegean before and after 7000 BC dispersal: defining patterning and variability. Neo-Lithics 1(16):32–41

    Google Scholar 

  • Çilingiroğlu A, Abay E (2005) Ulucak Höyük excavations: new results. Mediterr Archaeol Archaeometry 5(3):5–21

    Google Scholar 

  • Çilingiroglu C, Çakirlar C (2013) Towards configuring the neolithisation of Aegean Turkey. Doc Praehist 40:21–29

    Article  Google Scholar 

  • Çîlîngîroğlu A, Derîn Z, Abay E, Sağlamtîmur H, Kayan I (2004). Ulucak Höyük. Excavations Conducted Between 1995 and 2002. Ancient near eastern studies supplement series 15, Peeters, Leuven

  • Çilingiroğlu A, Çevik Ö, Çilingiroğlu Ç (2012) Ulucak Höyük: towards understanding the early farming communities of Middle West Anatolia: the contribution of Ulucak. In: Özdoğan M, Kuniholm P, Başgelen N (eds) The Neolithic in Turkey: new excavations and new research. Archaeology and Art Publications, Istanbul, pp 139–175

    Google Scholar 

  • Clementz MT, Fox-Dobbs K, Wheatley PV, Koch PL, Doak DF (2009) Revisiting old bones: coupled carbon isotope analysis of bioapatite and collagen as an ecological and palaeoecological tool. Geol J 44(5):605–620

    Article  Google Scholar 

  • Conolly J, Colledge S, Dobney K, Vigne J-D, Peters J, Stopp B, Manning K, Shennan S (2011) Meta-analysis of zooarchaeological data from SW Asia and SE Europe provides insight into the origins and spread of animal husbandry. J Archaeol Sci 38:538–545. https://doi.org/10.1016/j.jas.2010.10.008

    Article  Google Scholar 

  • Craig H (1961) Standard for reporting concentrations of deuterium and oxygen-18 in natural waters. Science 133:1833–1834

    Article  Google Scholar 

  • Dansgaard W (1964) Stable isotopes in precipitation. Tellus 16:436–468

    Article  Google Scholar 

  • Davis SJ (1987) The archaeology of animals. Routledge

  • Erdoğu B (2016) The Neolithic landscape and settlement of the Island of Gökçeada (Imbros, Turkey). In: Ghilardi M, Leandri F, Bloemendal J, Lespez L, Fachard S (eds) Géoarchéologie des îles de la Méditerranée. CNRS Editions Alpha, Paris, pp 89–94

    Google Scholar 

  • Galik A, Horejs B (2011) Çukuriçi Höyük—various aspects of its earliest settlement phase. In Krauss R (ed). Beginnings…new research in the appearance of the Neolithic between Northwestern Anatolia and the Carpathian Basin. Papers of the international workshop 8th–9th April 2009, Istanbul. Menschen-Kulturen-Traditionen 1. Rahen/Westfahlen. Leidorf, pp. 83–94

  • Gamba C, Jones ER, Teasdale MD, McLaughlin RL, Gonzalez-Fortes G, Mattiangeli V, Domboróczki L et al (2014) Genome flux and stasis in a five millennium transect of European prehistory. Nat Commun 5:5257

    Article  Google Scholar 

  • Geörg C (2013) Paläopopulationsgenetik von Schwein und Schaf in Südosteuropa und Transkaukasien: Von der Sesshaftigkeit zur komplexen Gesellschaft:/ Siedlung, Wirtschaft, Umwelt. Volume 9 of Menschen - Kulturen - Traditionen. Rahen/Westfahlen. Leidorf

  • Gillis R, Chaix L, Vigne J-D (2011) An assessment of morphological criteria for discriminating sheep and goat mandibles on a large prehistoric archaeological assemblage (Kerma, Sudan). J Archaeol Sci 38(9):2324–2339

    Article  Google Scholar 

  • Grant A (1982) The use of tooth wear as a guide to the age of domestic ungulates. In B. Wilson, C. Grigson, S. Payne S. (eds), Ageing and sexing animal bones from archaeological sites (British archaeological reports international series 109). Oxford: 91–107

  • Halstead P, Collins P, Isaakidou V (2002) Sorting the sheep from the goats: morphological distinctions between the mandibles and mandibular teeth of adult Ovis and Capra. J Archaeol Sci 29:545–553

    Article  Google Scholar 

  • Hedges REM, Stevens RE, Koch PL (2005) Isotopes in bones and teeth. In Leng MJ (ed). Isotopes in paleoenvironmental research pp. 117–145. Springer

  • Helmer D, Rocheteau M (1994) Atlas du squelette appendiculaire des principaux genres Holocenes de petits ruminants du nord de la Mediterranee et de procheorient (Capra, Ovis, Rupicapra, Capreolus, Gazella). In: Riches D’osteologie Animale Pour L’archeologie. Serie B: Mammiferes No. 4, pp. 3e21

  • Henton E, Meier-Augenstein W, Kemp HF (2010) The use of oxygen isotopes in sheep molars to investigate past herding practices at the Neolithic settlement of Çatalhöyük, central Anatolia. Archaeometry 52(3):429–449

    Article  Google Scholar 

  • Hoppe KA, Stover SM, Pascoe JR, Amundson R (2004) Tooth enamel biomineralization in extant horses: implications for isotopic microsampling. Palaeogeogr Palaeoclimatol Palaeoecol 206:355–365

    Article  Google Scholar 

  • Julien M-A, Bocherens H, Burke A, Drucker DG, Patou-Mathis M, Krotova O, Péan S (2012) Were European steppe bison migratory? 18O, 13C and Sr intra-tooth isotopic variations applied to a palaeoethological reconstruction. Quat Int 271:106–119

    Article  Google Scholar 

  • Kansa EC, Kansa SW, Watrall E (2011) Archaeology 2.0: new approaches to communication and collaboration. Cotsen Digital Archaeology Series

  • Kohn MJ (1996) Predicting animal δ18O: accounting for diet and physiological adaptation. Geochim Cosmochim Acta 60:4811–4829

    Article  Google Scholar 

  • Kohn MJ, Dettman DL (2007) Paleoaltimetry from stable isotope compositions of fossils. Rev Mineral Geochem 66:119–154

    Article  Google Scholar 

  • Krueger HW, Sullivan CH (1984) Models for carbon isotope fractionation between diet and bone. In: Turnlund J, Johnson PE (eds) Stable isotopes in nutrition. American Chemical Society, Washington, DC, pp 205–222

    Chapter  Google Scholar 

  • Lee-Thorp JA, Sealy JC, van der Merwe NJ (1989) Stable carbon isotope ratio differences between bone collagen and bone apatite, and their relationship to diet. J Archaeol Sci 16:585–599

    Article  Google Scholar 

  • Luz B, Kolodny Y, Horowitz M (1984) Fractionation of oxygen isotopes between mammalian bone phosphate and environmental drinking water. Geochim Cosmochim Acta 48:1689–1693

    Article  Google Scholar 

  • Makarewicz CA, Sealy J (2015) Dietary reconstruction, mobility, and the analysis of ancient skeletal tissues: expanding the prospects of stable isotope research in archaeology. J Archaeol Sci 56:146–158

    Article  Google Scholar 

  • Metzker ML (2010) Sequencing technologies—the next generation. Nat Rev Genet 11(1):31–46

    Article  Google Scholar 

  • Orlando L, Gilbert MT, Willerslev E (2015) Reconstructing ancient genomes and epigenomes. Nat Rev Genet 16(7):395–408

    Article  Google Scholar 

  • Orton D, Gaastra J, Vander Linden M (2016) Between the Danube and the deep blue sea: zooarchaeological meta-analysis reveals variability in the spread and development of Neolithic farming across the Western Balkans. Open Quaternary 2:6

    Article  Google Scholar 

  • Ottoni C, Flink LG, Evin A, Geörg C, De Cupere B, Van Neer W, Bartosiewicz L et al (2013) Pig domestication and human-mediated dispersal in western Eurasia revealed through ancient DNA and geometric morphometrics. Mol Biol Evol 30(4):824–832

    Article  Google Scholar 

  • Özdoğan M (2011) Archaeological evidence on the westward expansion of farming communities from eastern Anatolia to the Aegean and the Balkans. Curr Anthropol 52(4):415–430

    Article  Google Scholar 

  • Passey BH, Cerling TE (2002) Tooth enamel mineralization in ungulates: implications for recovering a primary isotopic time-series. Geochim Cosmochim Acta 66:3225–3234

    Article  Google Scholar 

  • Payne S (1972) Partial recovery and sample bias: the results of some sieving experiments. In: Higgs ES (ed) Papers in Economic Prehistory. Cambridge University Press, Cambridge, pp 49–64

    Google Scholar 

  • Payne S (1985) Morphological distinctions between the mandibular teeth of young sheep, Ovis, and goats, Capra. J Archaeol Sci 12:139–147

    Article  Google Scholar 

  • Pearson JA, Buitenhuis H, Hedges REM, Martin L, Russell N, Twiss KC (2007) New light on early caprine herding strategies from isotope analysis: a case study from Neolithic Anatolia. J Archaeol Sci 34:2170–2179

    Article  Google Scholar 

  • Pilaar Birch SE (2013) Stable isotope analysis in zooarchaeology. J Anthropol Archaeol Sci 5:81–83

    Article  Google Scholar 

  • Pilaar Birch SE, Cakırlar C. In preparation. Faunal and isotopic analyses at Neolithic Yenikapı

  • Pilaar Birch SE, Miracle PT, Stevens RE, O’Connell TC (2016) Reconstructing late Pleistocene/early Holocene migratory behavior of ungulates using stable isotopes and its effects on forager mobility. PLOS ONE (6):11, e0155714

  • Pilaar Birch SE, Atici L, Erdoğu B. In preparation. Sourcing wild and domestic fauna at Neolithic Uğurlu using stable isotope analysis

  • Prummel W, Frisch H-J (1986) A guide for the distinction of species, sex, and body size of sheep and goat. J Archaeol Sci 14:609–614

    Google Scholar 

  • Reade H, Stevens RE, Barker G, O’Connell TC (2015) Tooth enamel sampling strategies for stable isotope analysis: potential problems in cross-method data comparisons. Chem Geol 404:126–135

    Article  Google Scholar 

  • Scheu A (2012) Palaeogenetische Studien zur Populationsgeschichte von Rind und Ziege mit eienm Schwerpunkt auf dem Neolithikum in Südosteuropa: Von der Sesshaftigkeit zur komplexen Gesellschaft:/ Siedlung, Wirtschaft, Umwelt. Volume 4 of Menschen - Kulturen - Traditionen. Rahen/Westfahlen. Leidorf

  • Scheu A (2017) Neolithic animal domestication as seen from ancient DNA. Quat Int. https://doi.org/10.1016/j.quaint.2017.02.009

  • Scheu A, Hartz S, Schmölcke U, Tresset A, Burger J, Bollongino R (2008) Ancient DNA provides no evidence for independent domestication of cattle in Mesolithic Rosenhof, Northern Germany. J Archaeol Sci 35(5):1257–1264

    Article  Google Scholar 

  • Scheu A, Powell A, Bollongino R, Vigne J-D, Tresset A, Çakırlar C, Benecke N, Burger J (2015) The genetic prehistory of domesticated cattle from their origin to the spread across Europe. BMC Genet 16:54

    Article  Google Scholar 

  • Sykes N (2014) Hunting and hunting landscapes. In Encyclopedia of Global Archaeology (pp. 3592–3611). New York: Springer

  • van der Sluis LG, Hollund HI, Buckley M, De Louwd PGB, Rijsdijke KF, Kars H (2014) Combining histology, stable isotope analysis and ZooMS collagen fingerprinting to investigate the taphonomic history and dietary behaviour of extinct giant tortoises from the Mare aux Songes deposit on Mauritius. Palaeogeogr Palaeoclimatol Palaeoecol 416:80–91

    Article  Google Scholar 

  • von Holstein ICC, Ashby SP, van Doorn NL, Sachs SM, Buckley M, Meiric M, Barnes I, Brundlee A, Collins MJ (2014) Searching for Scandinavians in pre-Viking Scotland: molecular fingerprinting of Early Medieval combs. J Archaeol Sci 41:1–6

    Article  Google Scholar 

  • Zangrando AF, Tessone A, Ugan A, Gutierrez MA (2014) Applications of stable isotope analysis in zooarchaeology: an introduction. Int J Osteoarchaeol 24:127–133

    Article  Google Scholar 

  • Zazzo A, Balasse M, Patterson WP (2005) High-resolution δ13C intratooth profiles in bovine enamel: implications for mineralization pattern and isotopic attenuation. Geochim Cosmochim Acta 69:3631–3642

    Article  Google Scholar 

  • Zeder MA (2001) A metrical analysis of a collection of modern goats (Capra hircus aegargus and C. h. hircus) from Iran and Iraq: implications for the study of caprine domestication. J Archaeol Sci 28(1):61–79

    Article  Google Scholar 

  • Zeder MA (2006) Reconciling rates of long bone fusion and tooth eruption and wear in sheep (Ovis) and goat (Capra). Recent advances in ageing and sexing animal bones, pp87–118

  • Zeder MA, Lapham H (2010) Assessing the reliability of criteria used to post-cranial bones in sheep, Ovis, and goats, Capra. J Archaeol Sci 37:2887–2905

    Article  Google Scholar 

  • Zeder MA, Pilaar SE (2010) Assessing the reliability of criteria used to identify mandibles and mandibular teeth in sheep, Ovis, and goats, Capra. J Archaeol Sci 37:225–242

    Article  Google Scholar 

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Acknowledgements

This study has been funded by the Institute of Aegean Prehistory and the University of Groningen, Faculty of Arts.

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Pilaar Birch, S.E., Scheu, A., Buckley, M. et al. Combined osteomorphological, isotopic, aDNA, and ZooMS analyses of sheep and goat remains from Neolithic Ulucak, Turkey. Archaeol Anthropol Sci 11, 1669–1681 (2019). https://doi.org/10.1007/s12520-018-0624-8

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