Abstract
We applied a recently developed method of collagen peptide mass fingerprinting for taxonomic identification in faunal remains to 111 specimens from the Neolithic site of Domuztepe in southeastern Turkey. Advances on the published technique allow us to move beyond the key domesticated fauna (sheep, goat, pig and cattle) to include non-domesticates (deer and gazelle, as well as humans). As the animal economy of Domuztepe was dominated by domestic sheep and goats, a large number of the samples tested were morphologically indistinguishable sheep/goat remains. A number of samples represented elements that provide information on age (i.e., mandibles) or sex (pelvis); thus, this represents the first major study to attempt to improve kill-off profiles using collagen fingerprinting. We also show that the collagen extracted from dentine is equally amenable to the proposed methodology as bone collagen.
Similar content being viewed by others
References
Ambler RP, Daniel M (1991) Proteins and molecular palaeontology. Phil Trans Roy Soc Lond 333:381–389
Balasse M, Ambrose SH (2005) Distinguishing sheep and goats using dental morphology and stable carbon isotopes in C-4 grassland environments. J Archeol Sci 32:691–702
Bar-Gal GK, Ducos P, Horwitz LK (2003) The application of ancient DNA analysis to identify Neolithic caprinae: a case study from the site of Hatoula, Israel. Int J Osteoarch 13:120–131
Barker G, Chang C, Levy TE, Greenfield HJ (1988) On the Origins of Milk and Wool Production in the Old World. Curr Archaeol 29:743–748
Boessneck J (1969) Osteological differences between sheep (Ovis aries Linné) and goat (Capra hircus Linné). In: Brothwell D, Higgs E (eds) Science in Archaeology. Praeger, New York, pp 331–358
Buckley M, & Collins M, Submitted. Bone collagen, a molecular barcode for Quaternary fossils. J Quat Sci
Buckley M, & Kansa SW (2011) Domuztepe Collagen Fingerprinting Study, Open Context http://opencontext.org, California Digital Library Archival Identifier: ark:/28722/k23r0ps54 http://n2t.net/ezid/id/ark:/28722/k23r0ps54
Buckley M, Collins M, Thomas-Oates J, Wilson JC (2009) Species identification by analysis of bone collagen using matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry. Rapid Commun Mass Spectrom 23:3843–3854
Buckley M, Whitcher Kansa S, Howard S, Campbell S, Thomas-Oates J, Collins M (2010) Distinguishing between archaeological sheep and goat bones using a single collagen peptide. J Archeol Sci 37:13–20
Campbell S, Carter E, Healey E, Anderson S, Kennedy A, Whitcher S (1999) Emerging Complexity on the Kahramanmaras Plain, Turkey: the Domuztepe Project, 1995–1997. Am J Archaeol 103:395–418
Grant A (1982) The use of tooth wear as a guide to the age of domestic ungulates. Ageing and sexing animal bones from archaeological sites. BAR British Series, Oxford
Grine FE, Krause DW, Fosse G, Jungers WL (1987) Analysis of individual, intraspecific and interspecific variability in quantitative parameters of caprine tooth enamel structure. Acta Odontol Scand 45:1–23
Halstead P, Collins P, Isaakidou V (2002) Sorting the sheep from the goats: morphological distinctions between the mandibles and mandibular teeth of adult Ovis and Capra. J Archeol Sci 29:543–553
Helmer D (2000) Discrimination des genres Ovis et Capra à l’aide des prémolaires inférieures 3 et 4 et interpretation des ages d’abbatage: l’example de Dikili Tash (Grèce). J Mt Ecol 5:29–38
Loreille O, Vigne JD, Hardy C, Callou C, Treinen-Claustre F, Dennebouy N, Monnerot M (1997) First distinction of sheep and goat archaeological bones by the means of their fossil mtDNA. J Archeol Sci 24:33–37
Nielsen-Marsh C (2002) Biomolecules in fossil remains. The Biochemist, 12–14
Payne S (1969) A metrical distinction between sheep and goat metacarpals. In: Ucko PJ, Dimbleby GW (eds) The domestication and exploitation of plants and animals. Duckworth, London, pp 295–306
Payne S (1973) Kill-off patterns in sheep and goats: the mandibles from Asvan Kale. Anatol Stud 23:281–303
Payne S (1985) Morphological distinctions between the mandibular teeth of young sheep, Ovis and goats, Capra. J Archeol Sci 12:139–147
Peters J, von den Driesch A, & Helmer D (2005) The upper Euphrates-Tigris basin: cradle of agro-pastoralism? In: Vigne JD, Peters J, & Helmer D (eds) Proceedings of the 9th ICAZ Conference, Durham 2002, Durham Oxbow Books, Oxford. pp. 96–124
Prummel W, Frisch HJ (1986) A guide for the distinction of species, sex and body side in bones of sheep and goat. J Arch Sci 13:567–577
Robinson AB, & Rudd CJ (1974) Deamidation of glutaminyl and asparaginyl residues in peptides and proteins. In: Einsele G, Ricken W, & Seilacher A (eds) Current topics in cellular recognition, vol 8. Academic Press, New York. pp 247–294
Sherratt A (1983) The secondary exploitation of animals in the Old World. World Archaeol 15:90–104
Smith CI, Chamberlain AT, Riley MS, Stringer C, Collins MJ (2003) The thermal history of human fossils and the likelihood of successful DNA amplification. J Hum Evol 45:203–217
Zeder MA (1991) Feeding cities: specialized animal economy in the Ancient Near East (Smithsonian Series in Archaeological Inquiry). Smithsonian Institution, Washington
Zeder MA, Lapham HA (2010) Assessing the reliability of criteria used to identify postcranial bones in sheep, Ovis, and goats, Capra. J Arch Sci 37:2887–2905
Zeder MA, Pilaar SE (2010) Assessing the reliability of criteria used to identify mandibles and mandibular teeth in sheep, Ovis, and goats, Capra. J Archeol Sci 37:225–242
Acknowledgements
The authors would like to thank Malgosia Nowak-Kemp (Oxford Natural History Museum; samples 19437 and 19945) and Jerry Herman (Scotland Natural History Museum) for supplying modern specimens to sample and to Suellen Gauld for archaeological human and Josep Antoni Alcover for Myotragus samples. We would also like to acknowledge the Natural Environment Research Council for provision of a postdoctoral research grant NE/G000204/1 and a postdoctoral fellowship grant NE/H015132/1 for MB, the York Centre of Excellence in Mass Spectrometry (University of York), and the Michael Barber Center for Mass Spectrometry (University of Manchester). We also thank Stuart Campbell for access to and discussions about the site, and both Matthew Collins and Jane Thomas-Oates for advice throughout.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Buckley, M., Kansa, S.W. Collagen fingerprinting of archaeological bone and teeth remains from Domuztepe, South Eastern Turkey. Archaeol Anthropol Sci 3, 271–280 (2011). https://doi.org/10.1007/s12520-011-0066-z
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12520-011-0066-z