Abstract
Bio-mediated soil improvement has been recently introduced as a novel link between biotechnology and geotechnical engineering. Microbial induced carbonate precipitation (MICP) is one of the most promising bio-mediated methods which have been developed based on biologically driven urea hydrolysis. In the present study, an indigenous bacterium that can be used in MICP is identified in the native soil of Sirjan, Southern Iran. This bacterium, called Acinetobacter calcoaceticus Nima (ACN), was compared with the standard Sporosarcina pasteurii (SP), with respect to their performance in the improvement of the mechanical properties of biologically treated soil samples. During a 40-day period of the experiment, direct shear and shear wave velocity tests were conducted to investigate the influence of the treatments on soil properties. Also, stereo microscope images were used to investigate the precipitation on the inter-particle contacts. It was observed that the bio-mediated techniques can significantly improve the mechanical properties of the soil samples. For instance, while the shear wave velocity of the bacteria-free soil was measured as 175 m/s, SP-treated soil has the shear wave velocity of 526 m/s. This value was even higher for the ACN-treated samples (588 m/s) indicating that the local bacteria performed considerably better than the standard SP when used for the native soil.
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Sand-Poor
Sand-Mo (Swedish word for silt)
References
Acar YB, El-Tahir ET (1986) Low strain dynamic properties of artificially cemented sand. J Geotech Eng 112(11):1001–1015. https://doi.org/10.1061/(ASCE)0733-9410(1986)112:11(1001)
Akyol E, Bozkaya Ö, Dogan NM (2017) Strengthening sandy soils by microbial methods. Arab J Geosci 10(15):327. https://doi.org/10.1007/s12517-017-3123-9
Al-Salloum Y, Abbas H, Sheikh QI, Hadi S, Alsayed S, Almusallam T (2017) Effect of some biotic factors on microbial-induced calcite precipitation in cement mortar. Saudi J Biol Sci 24(2):286–294. https://doi.org/10.1016/j.sjbs.2016.01.016
Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ (1990) Basic local alignment search tool. J Mol Biol 215(3):403–410. https://doi.org/10.1016/S0022-2836(05)80360-2
ASTM (2012) Standard Practice for Classification of Soils for Engineering Purposes (Unified Classification System). ASTM Standard D 2487-11; ASTM International, West Conshohocken. https://doi.org/10.1520/D2487-11
Avseth P, Dvorkin J, Mavko G, Rykkje J (2000) Rock physics diagnostic of North Sea sands: link between microstructure and seismic properties. Geophys Res Lett 27(17):2761–2764. https://doi.org/10.1029/1999GL008468
Burbank M, Weaver T, Lewis R, Williams T, Williams B, Crawford R (2012) Geotechnical tests of sands following bio induced calcite precipitation catalyzed by indigenous bacteria. J Geotech Geoenviron 139(6):928–936. https://doi.org/10.1061/(ASCE)GT.1943-5606.0000781
Chapelle FH (2001) Ground-water Microbiology and Geochemistry. Wiley, New York
Chou CW, Seagren EA, Aydilek AH, Lai M (2011) Biocalcification of sand through ureolysis. J Geotech Geoenviron 137(12):1179–1189. https://doi.org/10.1061/(ASCE)GT.1943-5606.0000532
De Muynck W, De Belie N, Verstraete W (2010) Microbial carbonate precipitation in construction materials: a review. Ecol Eng 36(2):118–136. https://doi.org/10.1016/j.ecoleng.2009.02.006
Dejong JT, Mortensen BM, Martinez BC, Nelson DC (2010) Bio-mediated soil improvement. Ecol Eng 36(2):197–210. https://doi.org/10.1016/j.ecoleng.2008.12.029
Dhami NK, Sudhakara Reddy S, Mukherjee A (2013) Biomineralization of calcium carbonates and their engineered applications: a review. Front Microbiol 4:314. https://doi.org/10.3389/fmicb.2013.00314
Felsenstein J (1985) Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39(4):783–791
Grabiec AM, Starzyk J, Stefaniak K, Wierzbicki J, Zawal D (2017) On possibility of improvement of compacted silty soils using bio deposition method. Constr Build Mater 138:134–140. https://doi.org/10.1016/j.conbuildmat.2017.01.071
Hall TA (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. In: Nucleic acids symposium series (Vol. 41 No. 41 p. 95–98). Information Retrieval Ltd., London, pp c1979–c2000
Hamdan N, Kavazanjian E Jr, Rittmann BE, Karatas I (2017) Carbonate mineral precipitation for soil improvement through microbial denitrification. Geomicrobiol J 34(2):139–146. https://doi.org/10.1080/01490451.2016.1154117
He H, Zhang Z, Wang J, Li K (2013) Compressive properties of nano-calcium carbonate/epoxy and its fiber composites. Compos Part B 45(1):919–924. https://doi.org/10.1016/j.compositesb.2012.09.050
Ibragimov MN (2015) Design and implementation of soil stabilization by grout injection using hydrofracking technology. Soil Mechanics and Foundation Engineering 52(2):100–108. https://doi.org/10.1007/s11204-015-9313-0
Ismail AF, David LIB (2001) A review on the latest development of carbon membranes for gas separation. J Membr Sci 193(1):1–18. https://doi.org/10.1016/S0376-7388(01)00510-5
Jiang N, Soga K (2016) The applicability of microbially induced calcite precipitation (MICP) for internal erosion control in gravel–sand mixtures
Kano S, Moriwaki T, Ochi K (2017) A study on the bio-treatment technique of ground improvement with urease microorganisms which live in Japan. In: Geotechnical Hazards from Large Earthquakes and Heavy Rainfalls. Springer Tokyo, pp 441–448. https://doi.org/10.1007/978-4-431-56205-4_41
Karol RH (2003) Chemical grouting and soil stabilization, revised and expanded, vol 12. CRC Press
Kucharski ES, Cord-ruwisch R, Whiffin V, Al-thawadi SM (2008) United States patent
Law YF, Johnston KW, Routh HF, Cobbold RSC (1989) On the design and evaluation of a steady flow model for Doppler ultrasound studies. Ultrasound Med Biol 15(5):505–516. https://doi.org/10.1016/0301-5629(89)90103-8
Lowenstam HA, Weiner S (1989) On biomineralization. Oxford University Press
Neelands RJ, James AN (1963) Formulation and selection of chemical grouts, with typical examples of their field use. Grouts and Drilling Muds in Engineering Practice, 150–155
Pride SR (2005) Relationships between seismic and hydrological properties. In: Hydrogeophysics. Springer, Dordrecht, pp 253–290. https://doi.org/10.1007/1-4020-3102-5_9
Rajasekar A, Moy CK, Wilkinson S (2017) Stimulation of indigenous carbonate precipitating bacteria for ground improvement. In: IOP Conference Series: Earth and Environmental Science, Vol 68, No. 1. IOP Publishing, p 012010. https://doi.org/10.1088/1755-1315/68/1/012010
Sharma SS, Fahey M (2003) Degradation of stiffness of cemented calcareous soil in cyclic triaxial tests. J Geotech Geoenviron 129(7):619–629. https://doi.org/10.1061/(ASCE)1090-0241(2003)129:7(619)
Stotzky G (1997) Soil as an environment for microbial life. In: van Elsas JD, Trevors JT, Wellington EMH (eds) . Taylor & Francis, Modern soil microbiology, pp 1–20
Szabo TL, Wu J (2000) A model for longitudinal and shear wave propagation in viscoelastic media. J Acoust Soc Am 107(5):2437–2446
Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S (2011) MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance and maximum parsimony methods. Mol Biol Evol 28(10):2731–2739. https://doi.org/10.1093/molbev/msr121
Uzer AU (2016) Evaluation of freezing-thawing cycles for foundation soil stabilization. Soil Mech Found Eng 53(3):202–209. https://doi.org/10.1007/s11204-016-9386-4
Van Paassen LA, Harkes MP, Van Zwieten GA, Van der Zon WH, Van der Star WR, Van Loosdrecht MC (2009) Scale up of BioGrout: a biological ground reinforcement method. In: Proceedings of the 17th international conference on soil mechanics and geotechnical engineering. Lansdale, IOS Press, pp 2328–2333. https://doi.org/10.3233/978-1-60750-031-5-2328
Whiffin VS, van Paassen LA, Harkes MP (2007) Microbial carbonate precipitation as a soil improvement technique. Geomicrobiol J 24(5):417–423. https://doi.org/10.1080/01490450701436505
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Mohammadizadeh, M., Ajalloeian, R., Nadi, B. et al. Experimental study on soil improvement using local microorganisms. Arab J Geosci 13, 469 (2020). https://doi.org/10.1007/s12517-020-05450-3
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DOI: https://doi.org/10.1007/s12517-020-05450-3