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ZmFKBP20-1 improves the drought and salt tolerance of transformed Arabidopsis

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Abstract

FK506-binding proteins (FKBPs), which belong to the peptidyl-prolyl cis/trans isomerase superfamily, are involved in plant response to abiotic stresses. A number of FKBP family genes have been isolated in plants, but little has been reported of FKBP genes in maize. In this study, a drought-induced FKBP gene, ZmFKBP20-1, was isolated from maize and was characterized for its role in stress responses using gene expression, protein subcellular localization, transformation in Arabidopsis, expression patterns of the stress-responsive genes, and physiological parameter analysis. During drought and salt stresses, ZmFKBP20-1 transgenic Arabidopsis plants exhibited enhanced tolerance, which was concomitant with the altered expression of stress/ABA-responsive genes, such as COR15a, COR47, ERD10, RD22, KIN1, ABI1, and ABI2. The resistance characteristics of ZmFKBP20-1 overexpression were associated with a significant increase in survival rate. These results suggested that ZmFKBP20-1 plays a positive role in drought and salt stress responses in Arabidopsis and provided new insights into the mechanisms of FKBP in response to abiotic stresses in plants.

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Abbreviations

COR15a:

(cold-regulated gene 15a)

COR47:

(cold-regulated gene 47)

ERD10:

(early responsive to dehydration 10)

RD22:

(responsive to dehydration 22)

KIN1:

(kinase 1)

BI1:

(ABA-insensitive 1)

ABI2:

(ABA-insensitive 2)

RAB18:

(ABA-responsive gene 18)

Murashigeand Skoog:

(MS)

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Authors and Affiliations

  1. Maize Research Institute, Shandong Academy of Agricultural Sciences/National Engineering Laboratory of Wheat and Maize/Key Laboratory of Biology and Genetic Improvement of Maize in Northern Yellow-huai River Plain, Ministry of Agriculture, P.R. China, Jinan, 250100, China

    Yanli Yu, Yanjiao Li, Meng Zhao, Wencai Li, Qi Sun, Wenlan Li & Zhaodong Meng

  2. Key Laboratory of Agricultural Products Deep Processing Technology of Shandong; Institute of Agro-Food Science and Technology, Shandong Academy of Agricultural Sciences, Jinan, Shandong, 250100, P.R. China

    Fengjuan Jia

  3. Shandong Center of Crop Germplasm Resources/Shandong Academy of Agricultural Sciences, Jinan, 250100, P.R. China

    Nana Li

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  1. Yanli Yu
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  2. Yanjiao Li
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  3. Fengjuan Jia
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  4. Meng Zhao
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  9. Zhaodong Meng
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Yu, Y., Li, Y., Jia, F. et al. ZmFKBP20-1 improves the drought and salt tolerance of transformed Arabidopsis. J. Plant Biol. 60, 558–570 (2017). https://doi.org/10.1007/s12374-017-0262-1

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