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Growth, photosynthesis and water relations as affected by different drought regimes and subsequent recovery in Medicago laciniata (L.) populations

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Abstract

The aims of this study were to investigate the effects of water deficit and recovery on growth, photosynthesis and water relations in four Medicago laciniata populations from saharian (Ml-90), inferior arid (Ml-204), superior arid (Ml-306) and semi-arid (Ml-376) Tunisian regions. After 28 d of sowing with ample irrigation, the plants were subjected to 4 water regimes: optimal irrigation (100% of field capacity, FC), moderate drought (75% FC), severe drought (35% FC) and rewatering (plants submitted to 35% FC during 7 d, afterwards the plants were rewatered to 100% FC). Harvest was carried out after 28 d of treatments. The drought tolerance in M. laciniata populations was found to be increased particularly with increasing temperatures of collection site of the population. The Ml-204 and Ml-90 populations used mainly physiological strategies for survival under moderate water shortage. Higher severe drought tolerance in both signaled populations would be related to their lower photosynthesis metabolic impairment, relatively higher leaf RWC and greater osmotic potential decrease. The results suggest that plants with low values of leaf features are likely to maintain higher leaf RWC under sever drought. The largest decrease of osmotic potential was found associated with the solute accumulations such as proline and K+.

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References

  • Ackerly DD, Knight CA, Weiss SB, Barton K, Starmer KP (2002) Leaf size, specific leaf area and microhabitat distribution of chaparral woody plants: contrasting patterns in species level and community level analyses. Oecologia 130:449–457

    Article  Google Scholar 

  • Anyia AO, Herzog H (2004) Water-use efficiency, leaf area and leaf gas exchange of cowpeas under mid-season drought. Eur J Agron 20:327–339

    Article  Google Scholar 

  • Ashraf M (2003) Relationships between leaf gas exchange characteristics and growth of differently adapted populations of Blue panicgrass (Panicum antidotale Retz.) under salinity or waterlogging. Plant Sci 165:69–75

    Article  CAS  Google Scholar 

  • Ashraf M, Foolad MR (2007) Roles of glycine betaine and proline in improving plant abiotic stress resistance. Environ Exp Bot 59: 206–216

    Article  CAS  Google Scholar 

  • Badri M, Ilahi H, Huguet T, Aouani ME (2007). Quantitative and molecular genetic variation in sympatric populations of Medicago laciniata and M. truncatula (Fabaceae): relationships with ecogeographical factors. Genet Res 89:107–122

    Article  CAS  PubMed  Google Scholar 

  • Bajji M, Lutts S, Kinet JM (2001) Water deficit effects on solute contribution to osmotic adjustment as a function of leaf ageing in three durum wheat (Triticum durum Desf.) cultivars performing differently in arid conditions. Plant Sci 160:669–681

    Article  CAS  PubMed  Google Scholar 

  • Bates LS, Waldren RP, Teare D (1973) Rapid determination of free proline for water stress studies. Plant Soil 39:205–207

    Article  CAS  Google Scholar 

  • Bounejmate M, Robson AD, Beale PE (1992) Annual species in Marocco. II. Distribution in relation to soil and climate. Aust J Agri Res 43:751–763

    Article  Google Scholar 

  • Caspersen JP, Kobe RK (2001) Interspecific variation in sapling mortality in relation to growth and soil moisture. Oikos 92:160–168

    Article  Google Scholar 

  • Chartzoulakis K, Patakas A, Kofidis G, Bosabalidis A, Nastou A (2002) Water stress affects leaf anatomy, gas exchange, water relations and growth of two avocado cultivars. Sci Hort 95:39–50

    Article  CAS  Google Scholar 

  • Chaves MM, Maroco JP, Pareira JS (2003) Understanding plant responses to drought from 22 genes to the whole plant. Funct Plant Biology 30:239–264

    Article  CAS  Google Scholar 

  • Cifre J, Bota J, Escalona JM, Medrano H, Flexas J (2005) Physiological tools for irrigation scheduling in grapevine (Vitis vinifera L.): an open gate to improve water-use efficiency? Agric Ecosyst Environ 106:159–170

    Article  Google Scholar 

  • Cornic G (2000) Drought stress inhibits photosynthesis by decreasing stomatal aperture–not by affecting ATP synthesis. Trends Plant Sci 5:187–188

    Article  Google Scholar 

  • Cornish K, Radin JW, Turcotte EL, Lu Z, Zeiger E (1991) Enhanced photosynthesis and stomatal conductance of Pima cotton (Gossypium barbadense L.) bred for increased yield. Plant Physiol 97:484–489

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Erice G, Irigoyen JJ, Sánchez-Díaz M, Avice JJ, Ourry A (2007) Effect of drought, elevated CO2 and temperature on accumulation of N and vegetative storage proteins (VSP) in taproot of nodulated alfalfa before and after cutting. Plant Sci 172:903–12

    Article  CAS  Google Scholar 

  • Erice G, Louahlia S, Irigoyen JJ, Sanchez-Diaz M, Avice JC (2010) Biomass partitioning, morphology and water status of four alfalfa genotypes submitted to progressive drought and subsequent recovery. J Plant Physiol 167:114–120

    Article  CAS  PubMed  Google Scholar 

  • Ewing MA (1999) Annual pasture legumes: a vital component stabilizing and rehabilitating low-rainfall Mediterranean ecosystems. Arid Soil Res Rehab 13:327–342

    Article  Google Scholar 

  • Flexas J, Bota J, Cifre J, Escalona JM, Galmés J, Gulías J, Lefi E, Martínez-Cañellas SF, Moreno MT, Ribas-Carbó M, Riera D, Sampol B, Medrano H (2004) Understanding down-regulation of photosynthesis under water stress: future prospects and searching for physiological tools for irrigation management. Ann Appl Biol 144:273–283

    Article  Google Scholar 

  • Flexas J, Bota J, Escalona JM, Sampol B, Medrano H (2002) Effects of drought on photosynthesis in grapevines under field conditions: an evaluation of stomatal and mesophyll limitations. Funct Plant Biol 29:461–471

    Article  Google Scholar 

  • Garcia-Serrano H, Caño L, Escarré J, Fleck I, Sans FX (2009) Physiological comparison of alien Senecio inaequidens and S. pterophorus and native S. malacitanus: Implications for invasion. Flora 204:445–455

    Article  Google Scholar 

  • Hare PD, Cress WA (1997) Metabolic implications of stress-induced proline accumulation in plants. Plant Growth Regul 21:79–102

    Article  CAS  Google Scholar 

  • Hessini K, Ghandour M, Albouchi AA, Soltani A, Werner KH, Abdelly C (2008) Biomass production, photosynthesis, and leaf water relations of Spartina alterniflora under moderate water stress. J Plant Res 121:311–318

    Article  PubMed  Google Scholar 

  • Hoque MA, Okuma E, Akhter Banu MN, Nakamura Y, Shimoishi Y, Murata Y (2007) Exogenous proline mitigates the detrimental effects of salt stress more than exogenous betaine by increasing antioxidant enzyme activities. J Plant Physiol 164:553–561

    Article  CAS  PubMed  Google Scholar 

  • Jaleel CA, Gopi R, Manivannan P, Gomathinayagam M, Sridharan R, Panneerselvam R (2008) Antioxidant potential and indole alkaloid profile variations with water deficits along different parts of two varieties of Catharanthus roseus. Colloids Surf B Biointerfaces 62:312–318

    Article  CAS  PubMed  Google Scholar 

  • Javid MG, Akbari GA, Moradi F, Alahdadi I, Sorooshzadeh A (2010) Different Morphological, Physiological and Biochimical responses to Drought stress Tolerance in Cutleaf Medic (Medicago laciniata(L.) Mill). World Appl Sci J 11:938–948

    Google Scholar 

  • Jordan DC (1981) Reduction of the nodulation barrier in Medicago laciniata by alteration of the root temperature. Plant Soil 61:93–111

    Article  Google Scholar 

  • Lawlor DW, Cornic G (2002) Photosynthetic carbon assimilation and associated metabolism in relation to water deficits in higher plants. Plant Cell Environ 25:275–294

    Article  CAS  PubMed  Google Scholar 

  • Lehner B, Döll P, Alcamo J, Henrichs T, Kaspar F (2006) Estimating the impact of global change on flood and drought risk in Europe: a continental integrated analysis. Climatic Change 75:273–299

    Article  Google Scholar 

  • Lesins KA, Lesins I (1979) Genus Medicago (Leguminosae): A Taxogenetic Study. Dr. W. Junk Publishers, The Hague-Boston-London, pp 228

    Book  Google Scholar 

  • Liu F, Stützel H (2002) Leaf water relations of vegetable amaranth (Amaranthus spp.) in response to soil drying. Eur J Agron 16: 137–50

    Article  Google Scholar 

  • Loi A, Howieson JG, Cocks PS, Caredda S (1993) The adaptation of Medicago polymorpha to a range of edaphic and environmental conditions: effect of temperature on growth, and acidity stress on nodulation and nod gene induction. Aust J Exp Agri 33:25–30

    Article  Google Scholar 

  • Manivannan P, Jaleel CA, Somasundaram R, Panneerselvam R (2008) Osmoregulation and antioxidant metabolism in drought stressed Helianthus annuus under triadimefon drenching. C R Biol 331:418–425

    Article  CAS  PubMed  Google Scholar 

  • Marcelis LFM, Heuvelink E, Goudriaan J (1998) Modelling biomass production and yield of horticultural crops: a review. Sci Hortic 74:83–111

    Article  Google Scholar 

  • Martìnez JP, Lutts S, Schanck A, Bajji M, Kinet JM (2004) Is osmotic adjustment required for water stress resistance in the Mediterranean shrub Atriplex halimus L? J Plant Physiol 161:1041–1051

    Article  PubMed  Google Scholar 

  • Medrano H, Escalona JM, Bota J, Gulías J, Flexas J (2002) Regulation of photosynthesis of C3 plants in response to progressive drought: stomatal conductance as a reference parameter. Ann Bot (Lond) 89:895–905

    Article  CAS  Google Scholar 

  • Morgan JM (1984) Osmoregulation and water stress in higher plants. Annu Rev Plant Phys 35:199–319

    Article  Google Scholar 

  • Nautiyal PC, Nageswara Rao R, Joshi YC (2002) Moisture-deficitinduced changes in leaf water content, leaf carbon exchange rate and biomass production in groundnut cultivars differing in specific leaf area. Field Crop Res 74:67–79

    Article  Google Scholar 

  • Olsen JE, Bindi M (2002) Consequences of climate change for European agricultural productivity, land use and policy. Eur J Agron 16:239–262

    Article  Google Scholar 

  • Peoples MB, Baldock JA (2001) Nitrogen dynamics of pastures: nitrogen fixation inputs, the impact of legumes on soil nitrogen fertility, and the contributions of fixed nitrogen to Australian farming systems. Aust J Exp Agric 41:327–346

    Article  CAS  Google Scholar 

  • Peña-Rojas K, Aranda X, Fleck I (2004) Stomatal limitation to CO2 assimilation and down-regulation of photosynthesis in Quercus ilex resprouts in response toslowly-imposed drought. Tree Physiol 24:813–822

    Article  PubMed  Google Scholar 

  • Premachandra GS, Hahn DT, Rhodes D, Joly RJ (1995) Leaf water relations and solute accumulation in two-grain sorghum lines exhibiting contrasting drought tolerance. J Exp Bot 46:1833–1841

    Article  CAS  Google Scholar 

  • Premachandra GS, Saneoka H, Kanaya M, Ogata S (1989) Responses of relative growth rate, water relations and solute accumulation to increasing water deficits in maize. J Plant Physiol 135:257–260

    Article  CAS  Google Scholar 

  • Reeves TG, Ewing MA (1993) Is ley farming in Mediterranean zones just a passing phase? In M Baker, ed, XVII International Grassland Congress. Massey University, SIR Publisher, pp. 2169–2177

    Google Scholar 

  • Roberts SK (1998) Regulation of K+ channels in maize roots by water stress and abscisic acid. Plant Physiol 116:145–153

    Article  PubMed Central  CAS  Google Scholar 

  • Seemann JR, Critchley C (1985) Effects of salt stress on the growth, ion content, stomatal behaviour and photosynthetic capacity of a salt-sensitive species Phaseolus vulgaris L. Planta 164:151–162

    Article  CAS  PubMed  Google Scholar 

  • Shao HB, Chu LY, Abdul Jaleel C, Zhao CX (2008) Water-deficit stressinduced anatomical changes in higher plants. C R Biol 331: 215–225

    Article  PubMed  Google Scholar 

  • Sharp RE, Davies WJ (1989) Regulation of growth and development of plants growing with a restricted supply of water, In GJ Hamyln, TJ Flowers, MB Jones, ed, Plants under stress. Biochemistry, physiology and ecology and their application to plant improvement. Cambridge University Press, Cambridge, pp. 71–94

  • Small E, Jomphe M (1988) A synopsis of the genus Medicago (Leguminosae). Can J Bot 67:3260–3294

    Article  Google Scholar 

  • Sobrado MA, Turner NC (1986) Photosynthesis, dry matter accumulation and distribution in the wild sunflower Helianthus petiolaris and the cultivated sunflower Helianthus annuus as influenced by water deficits. Oecologia 69:181–187

    Article  Google Scholar 

  • Sánchez FJ, de Andrés EF, Tenorio JL, Ayerbe L (2004) Growth of epicotyls, turgor maintenance and osmotic adjustment in pea plants (Pisum sativum L.) subjected to water stress. Field Crops Res 86:81–90

    Article  Google Scholar 

  • Tanner JJ (2008) Structural biology of proline catabolism. Amino Acids 35:719–730

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Torrecillas A, Alarcün JJ, Domingo R, Planes J, Sánchez-Blanco MJ (1996) Strategies for drought resistance in leaves of two almond cultivars. Plant Sci 118:135–143

    Article  CAS  Google Scholar 

  • Turner NC (1997) Further progress in crop water relations. Adv Agron 58:293–338

    Article  Google Scholar 

  • Verbruggen N, Hermans C (2008) Proline accumulation in plants. Amino Acids 35:753–759

    Article  CAS  PubMed  Google Scholar 

  • Wang JR, Hawkins CDB, Letchford T (1998) Photosynthesis, water and nitrogen use efficiencies of four paper birch (Betula papyrifera) populations grown under different soil moisture and nutrient regimes. Ecol Manage 179:377–385

    Google Scholar 

  • Wang ZL, Huang BR, Xu QZ (2003) Effects of abscissic acid on drought responses of Kentucky bluegrass. J Am Soc Hortic Sci 128:36–41

    CAS  Google Scholar 

  • Wright GC, Nageswara Rao RC, Fraquhart GD (1994) water-use efficiency and carbon isotope discrimination in peanut under water-deficit conditions. Crop Sc 34:92–97

    Article  Google Scholar 

  • Wu F, Bao W, Li F, Wu N (2008) Effects of drought stress and N supply on the growth, biomass partitioning and water-use efficiency of Sophora davidii seedlings. Environ Exp Bot 63:248–55

    Article  CAS  Google Scholar 

  • Xu YL, Yu SW (1990) Energy consumption in plant adapting to salinity adverse habitat. Acta Phytophysiol Sin 6:70–73

    Google Scholar 

  • Yemm EW, Willis J (1954) The estimation of carbohydrates in plant extracts by anthrone. Biochem J 57:508–514

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Young RR, Croft PH, Sandral GA (1992) Variation in flowering times and agronomic characteristics of Medicago laciniata (L.) Miller collected from diverse locations in New South Wales. Austr J Exp Arig 32:59–63

    Article  Google Scholar 

  • Zhu JK (2003) Regulation of ion homeostasis under salt stress. Curr Opin Plant Biol 6:441–445

    Article  CAS  PubMed  Google Scholar 

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Authors and Affiliations

  1. Laboratory of extremophile plants, Centre of Biotechnology of Borj Cedria B.P. 901, 2050, Hammam-Lif, Tunisia

    Nasreddine Yousfi & Chedly Abdelly

  2. Laboratory of plant ecology, Faculty of Sciences of Tunis, Campus Universitaire, 2092, El Manar, Tunis, Tunisia

    Ncib Sihem

  3. Laboratory of Biochemistry and Genetic, Faculty of Sciences of Gafsa, Sidi Ahmed Zarrouk, 2112, Gafsa, Tunisia

    Amari Ramzi

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  1. Nasreddine Yousfi
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  2. Ncib Sihem
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Correspondence to Nasreddine Yousfi.

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Yousfi, N., Sihem, N., Ramzi, A. et al. Growth, photosynthesis and water relations as affected by different drought regimes and subsequent recovery in Medicago laciniata (L.) populations. J. Plant Biol. 59, 33–43 (2016). https://doi.org/10.1007/s12374-016-0422-8

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  • DOI: https://doi.org/10.1007/s12374-016-0422-8

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