Abstract
AtFes1A is a co-chaperone of heat shock protein 70 (Hsp70), which is a key component of plant molecular chaperone systems. Plants without AtFes1A generally present defective thermotolerance. However, the underlying mechanism of this molecule has remained elusive. In the present study, we used thermolabile firefly luciferase as a molecular reporter to evaluate the efficiency of the plant molecular chaperone system. Renaturation of firefly luciferase in atfes1a mutants lagged far behind that of wild-type (WT) plants. In contrast, in vitro AtFes1A did not affect renaturation of firefly luciferase, whereas Hsp70 did significantly. We propose that AtFes1A is indirectly involved in plant molecular chaperone systems.
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Fu, C., Zhang, J., Liu, X. et al. AtFes1A is essential for highly efficient molecular chaperone function in Arabidopsis. J. Plant Biol. 58, 366–373 (2015). https://doi.org/10.1007/s12374-015-0181-y
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DOI: https://doi.org/10.1007/s12374-015-0181-y