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GmREM1.1 and GmREM2.1, which encode the remorin proteins in soybean, have distinct roles during root nodule development

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Abstract

Remorin is involved in various biotic and abiotic stresses, including host-microbe interactions. To elucidate its roles during root nodule development, we isolated and characterized five remorin genes in soybean. The genes belonging to groups 1 and 2 were both expressed during root nodule development but with somewhat different temporal patterns. Using a biochemical assay, we showed that the homo-oligomerization of GmREM1.1 was mediated by the coiled-coil region in the C-terminal domain in combination with either the N-terminal or C-terminal anchor peptides (RemCA). Plasma membrane targeting was also sufficiently mediated by RemCA from GmREM1s but not from GmREM2.1. In addition, GmREM1.1 was highly expressed in the nodule primordia and inner cortex region of root nodules, whereas GmREM2.1 transcription was mainly detected in the infected cells. Moreover, RNAi-GmREM2.1 hairy roots showed significantly reduced nodule formation, but RNAi-GmREM1.1 had little effect on nodule formation. Taken together, our results suggest that GmREM1.1 and GmREM2.1, with different molecular features, function distinctively during root nodule development.

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Authors and Affiliations

  1. School of Biological Sciences, College of Natural Sciences, Seoul National University, Seoul, 151-747, Korea

    Seungmin Son, Chang Jae Oh, Ju-Hee Bae & Chung Sun An

  2. Division of Life Sciences, Korea Polar Research Institute (KOPRI), Songdo Techno Park, Incheon, 406-840, Korea

    Hyoungseok Lee

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  1. Seungmin Son
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  2. Chang Jae Oh
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  3. Ju-Hee Bae
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  5. Chung Sun An
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Correspondence to Chung Sun An.

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These two authors are equally contributed to this work

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Son, S., Oh, C.J., Bae, JH. et al. GmREM1.1 and GmREM2.1, which encode the remorin proteins in soybean, have distinct roles during root nodule development. J. Plant Biol. 58, 17–25 (2015). https://doi.org/10.1007/s12374-014-0273-0

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