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Genome-Wide Analysis of Genes Induced by Fusarium graminearum Infection in Resistant and Susceptible Wheat Cultivars

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Abstract

Fusarium head blight (FHB) caused by Fusarium graminearum is one of the most serious diseases in wheat (Triticum aestivum) and barley (Hordeum vulgare). Dahongmil is an elite Korean wheat cultivar with relatively high resistance to FHB. To identify differentially expressed genes in the resistant cultivar Dahongmil and the susceptible cultivar Urimil after inoculation of F. graminearum, we used the Affymetrix GeneChip® Wheat Genome Array to identify 328 ESTs that were differentially expressed in inoculated seedling tissues of the two cultivars. From these, we selected 16 induced genes and found that they have defense functions, such as genes encoding pathogen resistance proteins, oxidative stress-related proteins, metabolism, and proteins involved in defense mechanisms. To verify the DNA microarray results, we tested seven of these genes by semiquantitative RT-PCR and confirmed that these defense- and stress-related genes were expressed at much higher levels in the resistant Dahongmil cultivar. We next developed a hypothetical functional gene network and identified 89 interaction pairs mediated by four of the differentially expressed genes in the hypothetical network. We further refined the network by identifying nine genes showing significant up- or down-regulation after FHB challenge in the resistant cultivar and two genes having multiple interactions with queried proteins. We hope that the set of induced genes identified in this study can be used for development of new wheat and barley cultivars with improved resistance to FHB.

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Acknowledgments

This work was carried out with the support of Cooperative Research Program for Agriculture Science & Technology Development (PJ006662), Rural Development Administration, Republic of Korea.

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Authors and Affiliations

  1. Department of Plant Science, Plant Genomics and Breeding Institute, and Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul, 151-921, South Korea

    Sung-Hwan Cho & Nam-Chon Paek

  2. Department of Applied Biology and Chemistry, Seoul National University, Seoul, 151-921, South Korea

    Jungkwan Lee & Yin-Won Lee

  3. Department of Applied Biology, Dong-A University, Busan, 604-714, South Korea

    Jungkwan Lee

  4. Department of Plant Molecular Systems Biotechnology and Crop Biotech Institute, Kyung Hee University, Yongin, 446-701, South Korea

    Ki-Hong Jung

  5. Department of Rice and Winter Cereal Crop, National Institute of Crop Science, Rural Development Administration, Iksan, 570-080, South Korea

    Jong-Chul Park

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  1. Sung-Hwan Cho
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  2. Jungkwan Lee
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  3. Ki-Hong Jung
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  4. Yin-Won Lee
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Correspondence to Nam-Chon Paek.

Additional information

Sung-Hwan Cho, Jungkwan Lee, and Ki-Hong Jung contributed equally to this work.

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Fig. S1

Interaction network mediated by 89 up- and down-regulated genes in response to F. graminearum inoculation using the Cytoscape program (version 2.7). Green nodes indicate down-regulation in Dahongmil and red nodes indicate up-regulation. (JPEG 1773 kb)

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Cho, SH., Lee, J., Jung, KH. et al. Genome-Wide Analysis of Genes Induced by Fusarium graminearum Infection in Resistant and Susceptible Wheat Cultivars. J. Plant Biol. 55, 64–72 (2012). https://doi.org/10.1007/s12374-011-9190-7

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  • DOI: https://doi.org/10.1007/s12374-011-9190-7

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