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A salt stress-activated mitogen-activated protein kinase in soybean is regulated by phosphatidic acid in early stages of the stress response

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Abstract

Salt stress inhibits plant growth and development and plants activate kinase-dependent survival pathways in response to salt stress. However, the role of soybean mitogenactivated protein kinases (MAPKs) in salt stress response has yet to be characterized. In this study, we found that salt stress activates Glycine max MAP kinase 1 (GMK1), a soybean MAPK. The activity of GMK1 induced with increasing salt concentrations, up to 300 mM NaCl, after 5 min of the treatment and was regulated by post-translational modification. We found that mastoparan, a heteromeric G-protein activator, also activated GMK1, and that n-butanol, a phospholipase D inhibitor, and neomycin, a phospholipase C inhibitor, inhibited its activity. Moreover, GMK1 activity was reduced by suramin, a heteromeric G-protein inhibitor, and by two inhibitors of phosphatidic acid (PA) generation after 5 min of 300 mM NaCl treatment. Endogenous PA levels were highest 5 min after induction of salt stress, and exogenous PA directly activated GMK1. From these data, we propose that salt stress signaling is transduced from heteromeric G-protein to GMK1 via phospholipases in the early stages of the response to salt stress in soybean.

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Authors and Affiliations

  1. School of Biological Sciences, College of Natural Sciences, Seoul National University, Seoul, 151-747, Korea

    Jong Hee Im, Hyoungseok Lee, Jitae Kim, Ho Bang Kim & Chung Sun An

  2. Department of Chemistry, College of Natural Sciences, Seoul National University, Seoul, 151-747, Korea

    Kim Seyoung & B. Moon Kim

  3. Division of Life Sciences, Korea Polar Research Institute (KOPRI), Songdo Techno Park, Incheon, 406-840, Korea

    Hyoungseok Lee

  4. Department of Plant Biology, Cornell University, Ithaca, New York, 14853, USA

    Jitae Kim

  5. Life Sciences Research Institute, Biomedic, Inc., Bucheon, 420-852, Korea

    Ho Bang Kim

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  1. Jong Hee Im
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  2. Hyoungseok Lee
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  3. Jitae Kim
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  4. Ho Bang Kim
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  5. Kim Seyoung
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  6. B. Moon Kim
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Correspondence to Chung Sun An.

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Im, J.H., Lee, H., Kim, J. et al. A salt stress-activated mitogen-activated protein kinase in soybean is regulated by phosphatidic acid in early stages of the stress response. J. Plant Biol. 55, 303–309 (2012). https://doi.org/10.1007/s12374-011-0036-8

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  • DOI: https://doi.org/10.1007/s12374-011-0036-8

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