Abstract
Gibberellins (GAs) are endogenous hormones that play an important role in regulating plant stature by increasing cell division and promoting seed germination. The GA2-oxidase gene from Arabidopsis thaliana (AtGA2ox8) was introduced into Brassica napus L. by Agrobacterium-mediated floral-dip transformation with the aim of decreasing the amount of bioactive GA and hence reduced the plant height. As anticipated, the transgenic plant exhibited dwarf phenotype. Importantly, compared with the wild type, the transgenic plants had delayed the seed germination, increased the chlorophyll content (28.7–36.3%) and photosynthesis capacity (14.3–18.7%) in a single leaf. At the same time, the photosynthesis capacity of the whole plants was significantly enhanced (35.7–48.6%) due to the extra leaves and branches.
Similar content being viewed by others
References
Agharkar M, Lomba P, Altpeter F, Zhang HN, Kenworthy K, Lange T (2007) Stable expression of AtGA2ox1 in a low-input turfgrass (Paspalum notatum Flugge) reduces bioactive gibberellin levels and improves turf quality under field conditions. Plant Biotechnol J 5:791–801
Ariizumi T, Steber CM (2007) Seed Germination of GA-Insensitive sleepy1 Mutants Does Not Require RGL2 Protein Disappearance in Arabidopsis. The Plant Cell 19:791–804
Biemelt S, Tschiersch H, Sonnewald U (2004) Impact of altered gibberellin metabolism on biomass accumulation, lignin biosynthesis, and photosynthesis in transgenic tobacco plants. Plant Physiol 135:254–265
Dijkstra C, Adams E, Bhattacharya A, Page AF, Anthony P, Kourmpetli ÆS, Power JB, Lowe KC, Thomas SG, Hedden P, AL PÆ, Davey MR (2008) Over-expression of a gibberellin 2-oxidase gene from Phaseolus coccineus L. enhances gibberellin inactivation and induces dwarfism in Solanum species. Plant Cell Rep 27:463–470
Dunfield K, Srivastava S, Shah S, Kav NNV (2007) Constitutive expression of ABR17 cDNA enhances germination and promotes early flowering in Brassica napus. Plant Sci 173:521–532
Durley RC, Pharis RP (1973) Interconversion of gibberellin A4 to gibberellins Al and AM by dwarf rice, cultivar Tanginbozu. Planta 109:357–361
Finch-Savage WE, Leubner-Metzger G (2006) Seed dormancy and the control of germination. New Phytol 171:501–523
Huerta L, Forment J, Gadea J, Fagoaga C, Peña L, Pérez-amador M, García-martínez JL (2008) Gene expression analysis in Citrus reveals the role of gibberellins on photosynthesis and stress. Plant Cell Environ 31:1620–1633
Harberd NP, King KE, Carol P, Cowling RJ, Peng JR, Richards DE (1998) Gibberellin: inhibitor of an inhibitor of …? BioEssays 20(12):1001–1008
Hedden P, Graebe J (1985) Inhibition of gibberellin biosynthesis by paclobutrazol in cell-free homogenates of Cucurbita maxima endosperm and Malus pumila embryos. J Plant Growth Regul 4:111–122
Hedden P, Proebsting WM (1999) Genetic analysis of gibberellin biosynthesis. Plant Physiol 119:365–370
Hedden P, Phillips AL (2000) Gibberellin metabolism: new insights revealed by the genes. Trends Plant Sci 5:523–530
Huang J, Tang D, Shen Y, Qin BX, Hong LL, You AQ, Li M, Wang X, Yu HX, Gu MB, Cheng ZK (2010) Activation of gibberellin 2-oxidase 6 decreases active gibberellin levels and creates a dominant semi-dwarf phenotype in rice (Oryza sativa L.). J Genet Genomics 37:23–36
Iglesias-Fernández R, Matilla AJ (2010) Genes involved in ethylene and gibberellins metabolism are required for endosperm-limited germination of Sisymbrium oycinale L. seeds germination in Sisymbrium oycinale L. seeds. Planta 231:653–664
Kende H, Zeevaart JAD (1997) The five “classical” plant hormones. Plant Cell 9(1):197–1210
Kloosterman B, Navarro C, Bijsterbosch G, Lange T, Prat S, Visser RGF, Bachem CWB (2007) StGA2ox1 is induced prior to stolon swelling and controls GA levels during potato tuber development. Plant J 52:362–373
Koshioka M, Douglas TJ, Ernst D, Huber J, Pharis RP (1983) Metabolism of [3H]gibberellin A4 in somatic suspension cultures of anise. Phytochemistry 22:1577–1584
Kucera B, Cohn MA, Leubner-Metzger L (2005) Plant hormone interactions during seed dormancy release and germination. Seed Sci Res 15:281–307
Lee DJ, Zeevaart JAD (2005) Molecular cloning of GA 2-oxidase3 from spinach and its ectopic expression in Nicotiana sylvestris. Plant Physiol 138:243–254
Lo SF, Yang SY, Chen KT, Hsing YI, Zeevaart JAD, Chen LJ, Yu SM (2008) A novel class of gibberellin 2-oxidases control semidwarfism, tillering, and root development in rice. Plant Cell 20:2603–2618
Lichtenthaler HK (1987) Chlorophylls and carotenoids: pigments of photosynthetic biomembranes. Meth Enzymol 148:350–382
Lin JZ, Zhou B, Yang YZ, Mei J, Zhao XY, Guo XH, Huang XQ, Tang DY, Liu XM (2009) Piercing and vacuum infiltration of the mature embryo: a simplified method for Agrobacterium-mediated transformation of indica rice. Plant Cell Rep 28:1065–1074
Murray MG, Thompson WF (1980) Rapid isolation of high molecular weight plant DNA. Nucleic Acids Res 8:4321–4325
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays of tobacco tissue cultures. Physiol Plant 15:473–497
Nykiforuk CL, Johnson-Flanagan AM (1998) Low temperature emergence in crop plants: biochemical and molecular aspects of germination and early seedling growth. J Crop Prod 1:249–273
Norusis MJ (1993) Documentation SPSS for Windows. SPSS, Chicago
Oikawa T, Koshioka M, Kojima K, Yoshida H, Kawata M (2004) A role of OsGA20ox1, encoding an isoform of gibberellin 20-oxidase, for regulation of plant stature in rice. Plant Mol Biol 55:687–700
Olszewski N, Sun TP, Gubler F (2002) Gibberellin signaling: biosynthesis, catabolism, and response pathways. Plant Cell 14(suppl):S61–S80
Rodríguez-Gacio MC, Matilla AJ (2009) Seed dormancy and ABA signalling: the breakthrough goes on. Plant Signal Behav 4:1035–1048
Rood S, Hedden P (1994) Convergent pathways of gibberellin Al biosynthesis in Brassica. Plant Growth Regul 15:241–246
Rood SB, Pharis RP, Koshioka M (1983) Reversible conjugation of gibberellins in situ in maize. Plant Physiol 73:340–346
Rood SB, Pearce D, Williams PH, Pharis RP (1989) A gibberellin-deficient Brassica mutant—rosette. Plant Physio 189:482–487
Schomburg FM, Bizzell CM, Lee DJ, Zeevaart JAD, Amasino RM (2003) Overexpression of a novel class of gibberellin 2-oxidases decreases gibberellin levels and creates dwarf plants. Plant Cell 15:151–163
Sakamoto T, Kobayashi M, Ihoh H, Tagiri A, Kayano T, Tanaka H, Iwahori S, Matsuoka M (2001) Expression of a gibberellin 2-oxidase gene around the shoot apex is related to phase transition in rice. Plant Physiol 125:1508–1516
Sakamoto T, Miura K, Itoh H, Tatsumi T, Ueguchi-Tanaka M, Ishiyama K, Kobayashi M, Agrawal GK, Takeda S, Abe K, Miyao A, Hirochika H, Kitano H, Ashikari M, Matsuoka M (2004) An overview of gibberellin metabolism enzyme genes and their related mutants in rice. Plant Physiol 134:1642–1653
Sayed OH (2003) Chlorophyll fluorescence as a tool in cereal research. Photosynthetica 41:321–330
Sun TP, Gubler F (2004) Molecular mechanism of gibberellin signaling in plants. Annu Rev Plant Biol 55:197–223
Takahashi M, Kamiya Y, Takahashi N, Graebe JE (1986) Metabolism of gibberellins in a cell-free system from immature seeds of Phaseolus vulgaris L. Planta 168:190–199
Talon M, Zeevaart JA (1990) Gibberellins and stem growth as related to photoperiod in Silene armeria L. Plant Physiol 92:1094–1100
Thomas SG, Phillips AL, Hedden P (1999) Molecular cloning and functional expression of gibberellin 2-oxidases, multifunctional enzymes involved in gibberellin deactivation. Proc Natl Acad Sci USA 96:4698–4703
Wang H, Caruso LV, Downie AB, Perry SE (2004) The embryo MADS domain protein AGAMOUS-like 15 directly regulates expression of a gene encoding an enzyme involved in gibberellin metabolism. Plant Cell 16:1206–1219
Yamaguchi S, Kamiya Y, Sun T (2001) Distinct cell-specific expression patterns of early and late gibberellin biosynthetic genes during Arabidopsis seed germination. Plant J 28:443–453
Yamaguchi S, Kamiya Y, Nambara E (2007) Regulation of ABA and GA levels during seed development and germination in Arabidopsis. Blackwell, Oxford
Yamane H, Murofushi N, Osada H, Takahashi N (1977) Metabolism of gibberellins in early immature bean seeds. Phytochemistry 16:831–835
Yang J, Zhang J, Wang Z, Zhu Q, Wang W (2001) Hormonal changes in the grains of rice subjected to water stress during grain filling. Plant Physiol 127:315–323
Zeevaart JA, Gage DA, Talon M (1993) Gibberellin A1 is required for stem elongation in spinach. Proc Natl Acad Sci USA 90:7401–7405
Zhao XY, Yu XH, Foo E, Symons GM, Lopez J, Bendehakkalu KT, Xiang J, Weller JL, Liu XM, Reid JB, Lin CT (2007) A study of gibberellin homeostasis and cryptochrome-mediated blue light inhibition of hypocotyl elongation. Plant Physiol 145:106–111
Acknowledgments
This work was funded by the National 863 Plan of China (2007AA10Z127) and the National Natural Science Foundation of China (30800080).
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Zhou, B., Peng, D., Lin, J. et al. Heterologous Expression of a Gibberellin 2-Oxidase Gene from Arabidopsis thaliana Enhanced the Photosynthesis Capacity in Brassica napus L.. J. Plant Biol. 54, 23–32 (2011). https://doi.org/10.1007/s12374-010-9139-2
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12374-010-9139-2