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Flavonoid O-Diglucosyltransferase from Rice: Molecular Cloning and Characterization

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Abstract

Among more than 100 rice uridine diphosphate glycosyltransferases (UGTs), OsUGT-3 was selected as a candidate for producing flavonoid O-diglycosyltransferases based on phylogenetic analysis and molecular docking. This gene was functionally expressed in Escherichia coli. Analysis of kaempferol, luteolin, quercetin, and tricin reaction products using liquid chromatography-mass spectrometry revealed that these were diglucosylated. The glucosylation positions of kaempferol, which was the best substrate, were determined to be the 3- and 7-hydroxyl groups. This is the first flavonoid O-diglucosyltransferase described from rice.

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Acknowledgments

This work was supported by a Korea Research Foundation grant funded by the Korean Government (MOEHRD) (KRF-2005-214-00029).

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Authors and Affiliations

  1. Department of Bioscience and Biotechnology, Bio/Molecular Informatics Center, Konkuk University, Seoul, 143-701, South Korea

    Bong-Gyu Kim, Na Yeon Kim, Jeong Ho Kim, Youhoon Chong & Joong-Hoon Ahn

  2. Laboratory of Plant Pathology, United Graduate School and Faculty of Agriculture, Kagawa University, Miki, Kagawa, 761-0795, Japan

    Kazuya Akimitsu

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  1. Bong-Gyu Kim
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  2. Na Yeon Kim
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  4. Kazuya Akimitsu
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  6. Joong-Hoon Ahn
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Correspondence to Joong-Hoon Ahn.

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Kim, BG., Kim, N.Y., Kim, J.H. et al. Flavonoid O-Diglucosyltransferase from Rice: Molecular Cloning and Characterization. J. Plant Biol. 52, 41–48 (2009). https://doi.org/10.1007/s12374-008-9002-x

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  • DOI: https://doi.org/10.1007/s12374-008-9002-x

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