Abstract
Artificial pollination is one of the major constraints in date palm cultivation, while the unavailability or timely availability of pollen during the flowering period elevates the problem. One of the common practices is the use of stored pollen, but while stored at room temperature, pollen viability rapidly deteriorates after two to three months. In the present experiment, pollens were stored at ambient temperature and in a freezer at − 4 °C and a refrigerator at 5 °C using different storage containers for a year. The stored pollens were tested every month for 12 months for their pollen viability using acetocarmine as a staining agent. These stored pollens were then used for pollination, and on-farm fruit retention percentages were calculated. The best result for pollination was observed with the fresh pollen, while pollen stored at − 4 °C in a glass bottle gave the second-best results and can be used as an alternative in cases of pollen scarcity.
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References
Akond, A.S.M.G.M., C.T. Pounders, E.K. Blythe, and X. Wang. 2012. Longevity of crape-myrtle pollen stored at different temperatures. Scientia Horticulturae 139: 53–57. https://doi.org/10.1016/j.scienta.2012.02.021.
Al-Wusaibai, N.A., A.B. Abdallah, M.S. Al-Husainai, H. Al-Salman, and M. Elballaj. 2012. A comparative study between mechanical and manual pollination in two premier Saudi Arabian date palm cultivars. Indian Journal of Science and Technology 5 (4): 2487–2490. https://doi.org/10.17485/ijst/2012/v5i4.4.
Anushma, P.L., L. Vincent, P.E. Rajesekharan, and S. Ganeshan. 2018. Pollen storage studies in date palm (Phoenix dactylifera L.). International Journal of Chemical Studies 6 (5): 2640–2642.
Arnold, J.B. 2019. ggthemes: Extra Themes, Scales and Geos for ‘ggplot2’. R package version 4.2.0. https://CRAN.R-project.org/package=ggthemes.
Baidiyavadra, D.A., C.M. Muralidharan, and K.M. Sharma. 2019. Fresh date production in Kachchh (India): Challenges and prospects. Medicinal Plants 11 (3): 218–227. https://doi.org/10.5958/0975-6892.2019.00029.7.
Broussard, M.A., M. Coates, and P. Martinsen. 2023. Artificial pollination technologies: A review. Agronomy 13 (5): 1351. https://doi.org/10.3390/agronomy13051351.
Du, G., J. Xu, C. Gao, J. Lu, Q. Li, J. Du, M. Lv, and X. Sun. 2009. Effect of low storage temperature on pollen viability of fifteen herbaceous peonies. Biotechnology Reports 21: e00309. https://doi.org/10.1016/j.btre.2019.e00309.
Dutta, S.K., M. Srivastav, H. Rymbai, R. Chaudhary, A.K. Singh, A.K. Dubey, and K. Lal. 2013. Pollen-pistil interaction studies in mango (Mangifera indica L.) cultivars. Scientia Horticulturae 160: 213–221. https://doi.org/10.1016/j.scienta.2013.05.012.
El-Kadri, N., and M.B. Mimoun. 2020. In vitro germination of different date palm (Phoenix dactylifera L.) pollen sources from Southern Tunisia under the effect of three storage temperatures. International Journal of Fruit Science 20 (3): S1519–S1529. https://doi.org/10.1080/15538362.2020.1815116.
Iqbal, M., K. Usman, M. Munir, and M.S. Khan. 2018. Quantitative and qualitative characteristics of date palm cv. Gulistan in response to pollination times. Sarhad Journal of Agriculture 34 (1): 40–46. https://doi.org/10.17582/journal.sja/2018/34.1.40.46.
Johnson, D.V., J.M. Al-Khayri, and S.M. Jain. 2013. Seedling date palm (Phoenix dactylifera L.) as genetic resources. Emeritus Journal of Food and Agriculture 25 (11): 809–830. https://doi.org/10.9755/ejfa.v25i11.16497.
Kadri, K., M. Elsafy, S. Makhlouf, and M.A. Awad. 2021. Effect of pollination time, the hour of daytime, pollen storage temperature and duration of pollen viability, germinability and fruit set of date palm (Phoenix dactylifera L.) cv. “Deglet Nour.” Saudi Journal of Biological Sciences 29 (2): 1085–1091. https://doi.org/10.1016/j.sjbs.2021.09.062.
Kadri, K., A. Othmani, S. Makhloufi, M.S. Chebbi, A. Chokmani, and A. Touil. 2019. Contribution to the study of the effect of pollination mode on fruit set rate and yield in the date palm. International Journal of Agricultural Innovations and Research 7 (5): 533–537.
Karim, K., M.A. Awad, A. Manar, J. Monia, A. Karim, and E. Mohammed. 2022. Effect of flowering stage and storage conditions on pollen quality of six male date palm genotypes. Saudi Journal of Biological Sciences 29: 2564–2572. https://doi.org/10.1016/j.scienta.2021.12.038.
Kelen, M., and I. Demirtas. 2003. Pollen viability, germination capability and pollen production level of some grape varieties (Vitis vinifera L.). Acta Physiologiae Plantarum 25 (3): 229–233. https://doi.org/10.1007/s11738-003-0002-7.
Koubouris, G.G., I.T. Metzidakis, and M.D. Vasilakakis. 2009. Impact of temperature on olive (Olea europaea L.) pollen performance about relative humidity and genotype. Environmental and Experimental Botany 67: 209–214. https://doi.org/10.1016/j.envexpbot.2009.06.002.
Kumawat, P.K., R.S. Rathore, S. Kumar, G. Kumawat, and S. Rawar. 2022. Effect of stored pollen grains on quality and yield of date palm in western Rajasthan. The Pharma Innovation SP-11 (4): 1128–1132.
Maryam, M., J. Jaskani, B. Fatima, M.S. Haider, S.A. Naqvi, M. Nafees, R. Ahmad, and I.A. Khan. 2015. Evaluation of pollen viability in date palm cultivars under different storage temperatures. Pakistan Journal of Botany 47 (1): 377–381.
Mendiburu, F. 2019. Agricolae: Statistical procedures for Agricultural Research. R package version 1.3–1. https://CRAN.R-project.org/package=agricolae.
Mesnoua, M., M. Roumani, and A. Salem. 2018. The effect of pollen storage temperatures on pollen viability, fruit set and fruit quality of six date palm cultivars. Scientia Horitculturae 236: 279–283. https://doi.org/10.1016/j.scienta.2018.03.053.
Munir, M. 2019. Influence of liquid pollination technique on fruit yield and physicochemical characteristics of date palm cultivars Khadrawy and Zahidi. Journal of Biodiversity and Environmental Sciences 15: 41–49.
Muralidharan, C.M., C.N. Panchal, D.A. Baidiyavadra, K.M. Sharma, and P. Verma. 2020. Pistillate receptivity of date palm (Phoenix dactylifera L.) cv Barhee. Sugar Tech 22 (6): 1166–1169. https://doi.org/10.1007/s12355-020-00859-2.
Pinillos, V., and J. Cuevas. 2007. Artificial pollination in tree crop production. Horticultural Reviews 34: 239–276. https://doi.org/10.1002/9780470380147.ch4.
R Core Team. 2019. R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria. https://www.R-project.org/
Rezazadeh, R., H. Hassanzadeh, Y. Hosseini, Y. Karami, and R.R. Williams. 2013. Influence of pollen source on fruit production of date palm (Phoenix dactylifera L.) cv. Barhi in humid coastal regions of Southern Iran. Scientia Horticulturae 160: 182–188. https://doi.org/10.1016/j.scienta.2013.05.038.
Salomón-Torres, R., R. Krueger, J.P. García-Vázquez, R. Villa-Angulo, C. Villa-Angulo, N. Ortiz-Uribe, J.A. Sol-Uribe, and L. Samaniego-Sandoval. 2021. Date palm pollen: Features, production, extraction and pollination methods. Agronomy 11 (3): 504. https://doi.org/10.3390/agronomy11030504.
Shabana, H.R., A.A. Saeed, and A.H. Hamoodi. 2001. Determination of the optimal pollination period for Khalas date palm cultivar. In Proceedings of the second international conference on date palms, Al-Ain, United Arab Emirates, ed. A.A. Al-Badawy, 13–20. Al-Ain: UAE University.
Shaheen, M.A. 2004. Evaluation of date palm males using pollen viability and ultrastructure. Acta Horticulturae 632: 37–43.
Sharma, K.M., M. Kumar, C.M. Muralidharan, and R. Salomón-Torres. 2023. Date palm pollination management. In Date palm, ed. J.M. Al-Khayri, S.M. Jain, D.V. Johnson, and R.R. Krueger, 209–240. Wallingford: CABI. https://doi.org/10.1079/9781800620209.0007.
Sharma, K.M., C.M. Muralidharan, D.A. Baidiyavadra, K. Bardhan, and C.N. Panchal. 2021. Evaluation of potentiality of different adjuvants for date palm pollination and fruit set. Sugar Tech 23 (1): 139–145. https://doi.org/10.1007/s12355-020-00885-0.
Thompson, J.D., L.P. Rigney, K.M. Karoly, and B.A. Thomson. 1994. Pollen viability, vigor, and competitive ability in Erythronium grandiflorum (Liliaceae). American Journal of Botany 81: 1257–1266.
Wickam, H. 2017. tidyverse: Easily Install and Load the ‘Tidyverse’. R package version 1.2.1. https://CRAN.R-project.org/package=tidyverse.
Wickam, H. 2016. ggplot2: Elegant graphics for data analysis. New York: Springer.
Yao, S.D.M., J.L.M. Konan, R.S. Sie, A.R. Assa, and K. Allou. 2010. Effet de la duree de conservation sur la qualite du pollen en production de semences chez le cocotier (Cocos nucifera L.). Sciences & Nature 7: 87–96. https://doi.org/10.4314/scinat.v7i1.59938.
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KMS conducted, analyzed and wrote the manuscript; DAB conducted and supported in experimentation; CMM formulated and facilitated the experiment; CNP and PV helped in the formulation of the experiment. All the authors have reviewed the manuscript before submission.
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Sharma, K.M., Baidiayavadra, D.A., Muralidharan, C.M. et al. Effect of Storage Temperature and Containers on Date Palm (Phoenix dactylifera L.) Pollen Viability and Post-storage Pollination. Sugar Tech (2024). https://doi.org/10.1007/s12355-024-01415-y
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DOI: https://doi.org/10.1007/s12355-024-01415-y