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The Effect on Ethanol Production Using Red Seaweed Extracts in Sugarcane Juice Treatment

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Abstract

The present study assessed the clarification of sugarcane juice using different flocculants and their effect on ethanol yield. The extracted sugarcane samples (CTC072361—harvest 2022/2023) were adjusted to 16% Brix, and calcium hydroxide (56 g L−1) was added with pH 7.0 ± 0.4 and boiled. The clarification test was done, using the anionic synthetic polymer, commercial organic flocculant and red seaweed extract (RSE—Kappaphycus alvarezii) for sedimentation of impurities. After a retention time of 20 min, the clarified broths were collected by siphoning and analyzed for Brix, total reducing sugars (TRS), pH, total acidity, and total phenolic compounds. The extracted sugarcane juice was analyzed for Brix, TRS, reducing sugars, Pol, pH, purity, acidity, ash, total phenolic compounds, turbidity, and starch content. The clarified juices were standardized to obtain the wort and analyzed for TRS and total acidity. The worts were processed for fermentation process, using CAT-1 yeast (30 g L−1). After fermentation (Brix ≤ 1%), the wine samples were collected and analyzed for total residual reducing sugars, pH, total acids, alcohol content and glycerol, with subsequent calculation of fermentation efficiency. The use of red algae extracts (Kappaphycus alvarezii) was found efficient as a bioflocculant for removing impurities in sugarcane juice and promoted a similar clarification process with synthetic and organic flocculants. The use of red seaweed resulted in as fermentation efficiency 93.27% and represented up to 3.4% higher than the other treatments.

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Acknowledgements

The authors would like to thank the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) and the Fundação de Amparo à Pesquisa do Estado de Minas Gerais (Fapemig) for the research grant granted (Process APQ-03146-21), as well as the University of the State of Minas Gerais (UEMG-Frutal) for helping with the project and the company Tanac® for providing the material to carry out the study.

Funding

This research was conducted using founds from University of Minas Gerais State.

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Authors and Affiliations

  1. Department of Agronomy and Biological Sciences, Universidade do Estado de Minas Gerais, Frutal, MG, Brazil

    Giovanni Uema Alcantara, Maurício Bonatto Machado de Castilhos & Gustavo Henrique Gravatim Costa

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  1. Giovanni Uema Alcantara
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  2. Maurício Bonatto Machado de Castilhos
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  3. Gustavo Henrique Gravatim Costa
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Contributions

GUA did material preparation, data collection, analysis and first draft of the manuscript, reviewed and edited the manuscript. MBMdC done experimental design; GHGC and MBMdC performed data collection, analysis, first draft of the manuscript, reviewed and edited the manuscript. All authors read and approved this final manuscript.

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Correspondence to Giovanni Uema Alcantara.

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The authors declare that they have no conflict of interest, financial interests or any personal relationships that could influence in this present research.

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The authors inform that this research followed principles of ethical and professional conduct. The manuscript was not submitted to another journal for simultaneous consideration. The data are original and they are not published elsewhere in any form or language (partially or in full). The results were presented clearly, honestly, and without fabrication, falsification or inappropriate data manipulation.

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Alcantara, G.U., de Castilhos, M.B.M. & Costa, G.H.G. The Effect on Ethanol Production Using Red Seaweed Extracts in Sugarcane Juice Treatment. Sugar Tech (2024). https://doi.org/10.1007/s12355-024-01389-x

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