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Proteome Based Comparative Investigation of a High Sucrose Sugarcane Mutant in Contrast to the Low Sucrose Mother Variety by Using TMT Quantitative Proteomics

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Abstract

Sugarcane (Saccharum sp. hybrid) is a significant crop in the world’s economy and fulfills 80% of the world's sugar production. To meet the rising sugar requirement, it is emphasized to enhance the cane sugar yield. In the present study, the TMT quantitative proteomic approach was applied to find the proteins associated with sucrose accumulation in a high sucrose sugarcane mutant GXB9 compared to its low sucrose mother variety B9. A total of 27,922 proteins were obtained, and 7484 proteins received functional annotations on blasting in COG, GO, NR, Pfam, and KEGG databases. A sum of 3,102 differentially expressed proteins (DEPs) was recognized when the immature and maturing internodes of GXB9 were compared with B9. Sucrose phosphate synthase (SPS), invertases (CWIN, NIN1, CINV2), and sucrose synthase (SuSy) are significant proteins involved in sucrose metabolism and accumulation in sugarcane. This study discovered that the DEPs of SPS (SPS2, SPS5) were upregulated, while SuSy, CWIN, NIN1, CINV2 were downregulated in the maturing internodes, and seven DEPs of trehalose phosphate synthase such as TPS6, TPS7, TPS9, TPS9, and trehalose phosphate phosphatase were downregulated in the immature and maturing internodes of GXB9 compared with B9. The result of our exploration would mediate progression in sugarcane varieties by concentrating on the manipulation of proteins associated with sucrose synthesis, metabolism, and accumulation through the use of advanced molecular approaches.

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Acknowledgements

The authors are thankful to Shanghai Majorbio Bio-pharm Biotechnology Co., Ltd. http://www.majorbio.com/ (Shanghai, China) for the sequencing, transcriptomic, and bioinformatics analysis.

Funding

This work was financially supported by Guangxi Special Fund for Scientific Base and Talent (GKAD17195100), Fund for Guangxi Innovation Teams of Modern Agriculture Technology (gjnytxgxcxtd-03–01), and Fund of Guangxi Academy of Agricultural Sciences (2021YT011).

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Authors and Affiliations

  1. College of Agricultural, Guangxi University, Nanning, 530004, China

    Qaisar Khan, Ying Qin, Dao-Jun Guo, Zhen Lu, Xian-Qiu Xie, Li-Tao Yang, Yong-Xiu Xing & Yang-Rui Li

  2. Guangxi Key Laboratory of Sugarcane Genetic Improvement, Key Laboratory of Sugarcane Biotechnology and Genetic Improvement (Guangxi), Ministry of Agriculture and Rural Affairs, Sugarcane Research Institute of Guangxi Academy of Agricultural Sciences, Sugarcane Research Center of Chinese Academy of Agricultural Sciences, Nanning, 530007, China

    Qiang Liang, Xiu-Peng Song & Yang-Rui Li

Authors
  1. Qaisar Khan
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  2. Ying Qin
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  3. Dao-Jun Guo
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  4. Zhen Lu
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  5. Xian-Qiu Xie
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  6. Li-Tao Yang
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  7. Qiang Liang
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  8. Xiu-Peng Song
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  9. Yong-Xiu Xing
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  10. Yang-Rui Li
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Contributions

Q.K., Y.Q., L.Z., X.X., D.J.G., and Q.L. performed the experiment and did bioinformatics analysis. L.T.Y., X.P.S., Y.X.X., and Y.R.L. supervised the project. Q.K., L.T.Y., X.P.S., Y.X.X, and Y.R.L. designed the study. Q.K., X.P.S., Y.X.X, and Y.R.L. participated in writing the manuscript. All authors have read and agreed to the published version of the manuscript.

Corresponding authors

Correspondence to Xiu-Peng Song, Yong-Xiu Xing or Yang-Rui Li.

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The authors declare that they have no conflict of interest.

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The data that support the findings of this study are available in the supplementary material of this article.

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Khan, Q., Qin, Y., Guo, DJ. et al. Proteome Based Comparative Investigation of a High Sucrose Sugarcane Mutant in Contrast to the Low Sucrose Mother Variety by Using TMT Quantitative Proteomics. Sugar Tech 24, 1246–1259 (2022). https://doi.org/10.1007/s12355-022-01160-0

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  • DOI: https://doi.org/10.1007/s12355-022-01160-0

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