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Xylooligosaccharide Production from Sugarcane Bagasse using Recombinant Endoxylanase of Bacillus Halodurans

  • S.I. : Diversification of Sugar Crops for Value Addition
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Abstract

Xylooligosaccharides (XOSs) can be manufactured from sugarcane bagasse as a value-added product in the sugar industry. To produce XOS, xylan was extracted from sugarcane bagasse by using sodium hydroxide, precipitated in ethanol, and subjected to enzymatic hydrolysis by using endoxylanase to form xylooligomers. We constructed a recombinant strain of Escherichia coli harbouring the recombinant plasmid containing a gene encoding endoxylanase modified from GH10 xylanase of alkaliphilic Bacillus halodurans BCRC 910,501. Results from the flask culture showed that in terms of activity, 80.43 ± 6.36% of recombinant proteins were secreted to the medium under the induction of 0.1 mM isopropyl β-D-1-thiogalactopyranoside (IPTG) for 18 h at 30 °C. The activity concentration in the medium was 20.04 ± 4.11 U/mL. When this strain was fed-batch cultivated to high cell density in a bench-top fermenter, 89.13 ± 0.66% of the recombinant enzymes were found in the medium, and the active protein concentration was 45.9 ± 7.32 U/mL. Thus, the recombinant E. coli has the potential for large-scale production of endoxylanase. A 24-h enzyme reaction yielded 44.91% (w/w) of XOS from xylan. Among the total soluble oligomers in the product, 40.6% (w/w) were xylobiose and xylotriose.

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Fig. 1

modified from the endoxylanase GH10 of B. halodurans BCRC 910,501

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Authors and Affiliations

  1. Department of Chemical Engineering, National Chung Cheng University, 168 University Road, Minhsiung, Chiayi, 621, Taiwan

    Ying-Hsuan Tseng & Wen-Chien Lee

  2. Mitr Phol Sugarcane Research Center Co., Ltd., 399 Moo 1 Chumpae-Phukieo Road, Khoksa-at, Phukieo, Chaiyaphum, 36110, Thailand

    Kridsana Krisomdee, Waranya Natesuntorn, Sunisa Chatsurachai & Klanarong Sriroth

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  1. Ying-Hsuan Tseng
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  2. Wen-Chien Lee
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  3. Kridsana Krisomdee
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Tseng, YH., Lee, WC., Krisomdee, K. et al. Xylooligosaccharide Production from Sugarcane Bagasse using Recombinant Endoxylanase of Bacillus Halodurans. Sugar Tech 24, 1029–1036 (2022). https://doi.org/10.1007/s12355-021-01096-x

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