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Glucanolytic Rhizobacteria Produce Antifungal Metabolites and Elicit ROS Scavenging System in Sugarcane

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Abstract

Plant growth-promoting bacteria have broad range usage in agriculture as an alternative to chemical fertilizers and fungicides. Their survival and persistency in the plant vicinity depend upon the production of secondary metabolites. In present study, 45 bacteria were isolated from the rhizosphere and endosphere of different sugarcane varieties growing at the different farmer’s fields of Punjab, Pakistan. Out of 45 isolates, 3 were able to produce hydrolytic enzyme glucanase. The glucanase-producing ability of bacterial strains was variable with solubilization zone 5.5–13.8 mm of different substrates. Dinitrosalicylic acid (DNS) quantification depicted the production of glucanase (0.1–0.3 U/mL of culture filtrate) by the antagonistic strains. All the glucanase-producing bacteria significantly inhibited the economically important pathogens of sugarcane, i.e., Fusarium moniliforme (45–56%) and Colletotrichum falcatum (52–63%), and pathogens of other crops, i.e., Fusarium oxysporum (58–63%), Rhizoctonia solani (42–53%) and Macrophomina phaseolina (53–61%). The glucanase-producing bacteria significantly induced the activities of enzymes involved in ROS scavenging, viz. SOD, POD, CAT and PPO by 1.4–2.0-fold. The glucanase-producing bacteria MAZ-3SR was identified as Bacillus amyloliquefaciens, while MAZ-10SR and MAZ-29SR were identified as Bacillus subtilis by 16S rDNA sequence comparision. These glucanolytic bacteria could be effectively used in sugarcane disease management and provide an effective way of enumerating the bioantagonists from sugarcane rhizosphere.

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Acknowledgements

The authors would like to thank Higher Education Commission (HEC), Pakistan, for providing funds under the research Grant (20-2991). We would also acknowledge the fellows for their motivation and help in the work. A special thanks to colleagues who helped in improving the English of manuscript .We are also thankful to Mr. Habib Ullah for helping in molecular work.

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  1. Shahid Afghan

    Present address: Sugarcane Research and Development Board, Punjab, Pakistan

Authors and Affiliations

  1. Department of Biosciences, COMSATS University Islamabad (CUI), Park Road, Islamabad, Pakistan

    Muhammad Atif Zia, Humaira Yasmin, Faluk Shair, Zahra Jabeen, Saqib Mumtaz, Zafar Hayat, Fauzia Yusuf Hafeez & Muhammad Nadeem Hassan

  2. Fatima Sugar Research & Development Centre, Fatima Sugar Mills Limited, Kot Addu, Pakistan

    Syed Zia ul Husnain Shah

  3. Shakarganj Sugar Research Institute, Toba Road, Jhang, Pakistan

    Shahid Afghan

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  1. Muhammad Atif Zia
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Zia, M.A., Yasmin, H., Shair, F. et al. Glucanolytic Rhizobacteria Produce Antifungal Metabolites and Elicit ROS Scavenging System in Sugarcane. Sugar Tech 21, 244–255 (2019). https://doi.org/10.1007/s12355-018-0654-7

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