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Isolation, Characterization and Promoter Analysis of Cell Wall Invertase Gene SoCIN1 from Sugarcane (Saccharum spp.)

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Abstract

Cell wall invertase (CIN) plays an important role in carbohydrate partitioning and regulation of sink-source interaction. In this study, a full length cDNA encoding CIN gene was cloned by RT-PCR and RACE-PCR from sugarcane. The open reading frame of 1731 bp encodes a protein of 577 amino acids with a predicted molecular mass of 141.04 kDa and theoretical PI of 4.67. A size of 4,537 bp DNA encoding SoCIN1 was isolated, which contains seven exons and six introns. Using genome walking approach, a promoter sequence of 974 bp of SoCIN1 was isolated, and prediction with PlantCARE and PLACE software revealed that it has several key cis-regulatory elements known to be involved in various biotic and abiotic plant stresses. Relative expression in specific tissues was done using qRT-PCR, and the results demonstrated that SoCIN1 expression level was higher in the immature leaves and maturing leaves than the matured leaves and internodes at elongation stage and processing maturing stage. The conditions of 15 % PEG and 6 °C could have a similar function for inducing the expression of SoCIN gene in leaves, while 15 % PEG, 100 mM NaCl and 6 °C could induce the expression of SoCIN gene in roots.

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Acknowledgments

This present study was supported by the grants from the National High Technology Research and Development Program (“863” Program) of China (2013AA102604), International Scientific Cooperation Program of China (2013DFA31600), Guangxi Special Funds for Bagui Scholars’s and Distinguished Experts, and Guangxi Natural Science Fund (2011GXNSFF018002, 2013NXNSFAA019073).

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Authors and Affiliations

  1. State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresource, College of Agriculture, Guangxi University, 100 East Daxue Road, Nanning, 530005, China

    Jun-Qi Niu, Ai-Qin Wang, Jing-Li Huang & Li-Tao Yang

  2. Key Laboratory of Sugarcane Biotechnology and Genetic Improvement (Guangxi), Ministry of Agriculture, Nanning, People’s Republic of China

    Li-Tao Yang & Yang-Rui Li

  3. Sugarcane Research Center, Chinese Academy of Agricultural Sciences-Guangxi Academy of Agricultural Sciences, 174 East Daxue Road, Nanning, 530007, China

    Li-Tao Yang & Yang-Rui Li

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  1. Jun-Qi Niu
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  4. Li-Tao Yang
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Correspondence to Li-Tao Yang or Yang-Rui Li.

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Niu, JQ., Wang, AQ., Huang, JL. et al. Isolation, Characterization and Promoter Analysis of Cell Wall Invertase Gene SoCIN1 from Sugarcane (Saccharum spp.). Sugar Tech 17, 65–76 (2015). https://doi.org/10.1007/s12355-014-0348-8

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