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Osmolytes Accumulation, Cell Membrane Integrity, and Antioxidant Enzymes in Sugarcane Varieties Differing in Salinity Tolerance

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Abstract

Osmolyte accumulation (proline and glycine–betaine), chlorophyll content, nitrate reductase (NR) activity and antioxidative system in terms of superoxide dismutase (SOD), ascorbate peroxidase (APX), catalase (CAT) and malondialdehyde content was studied in two sugarcane varieties (CoM-265 and CoC-671) differing in salt tolerance. The result clearly indicated higher level of osmolytes like proline and glycine–betaine in CoM-265, a salt tolerant variety. The reduction in chlorophyll content and NR activity was less in salt tolerant sugarcane variety CoM-265 than that of CoC-671, a salt susceptible variety. Higher activity of SOD, APX and CAT with lower LPO content in salt tolerant (CoM-265) variety compared to salt susceptible (CoC-671) variety, suggests that antioxidant enzymes protect cell from oxidative burst. Proline and glycine–betaine protect cell membrane integrity by maintaining higher chlorophyll and NR activity by scavenging ROS under salt stress.

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Abbreviations

SOD:

Superoxide dismutase

APX:

Ascorbate peroxidase

MDA:

Malondialdehyde

NBT:

Nitro blue tetrazolium

TBA:

Thiobarbituric acid

ROS:

Reactive oxygen species

NRA:

Nitrate reductase

LPO:

Lipid peroxidation

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Acknowledgments

We wish to thank Sugarcane Specialist, CSRS, Padegaon under M.P.K.V. Rahuri, Dist-Ahmednagar, Maharashtra, India for supply to sugarcane varieties for this study.

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Authors and Affiliations

  1. Department of Biochemistry, Mahatma Phule Krishi Vidyapeeth, Rahuri, Maharashtra, 413722, India

    R. D. Satbhai & R. M. Naik

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  1. R. D. Satbhai
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  2. R. M. Naik
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Correspondence to R. M. Naik.

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Satbhai, R.D., Naik, R.M. Osmolytes Accumulation, Cell Membrane Integrity, and Antioxidant Enzymes in Sugarcane Varieties Differing in Salinity Tolerance. Sugar Tech 16, 30–35 (2014). https://doi.org/10.1007/s12355-013-0243-8

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