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Phenotypic and biochemical responses of sugarcane cultivars to glyphosate application

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Abstract

Sugarcane cultivars show varying degrees of herbicide susceptibility, with herbicide effects ranging from no injury to total plant eradication. In this study, phenotypic and biochemical responses to glyphosate were evaluated in three sugarcane cultivars. Herbicide tolerance of the three cultivars (IACSP93-3046, IACSP94-4004 and RB72454) was tested in a greenhouse experiment using a completely randomized design. The experiment consisted of four replications of treatments in a factorial arrangement of 3 cultivars × 6 glyphosate dosages (0, 1,440, 2,160, 2,880, 3,600 and 4,320 g a. e. ha−1). Intoxication symptoms, based on a percentage scale ranging from 0 (no injury) to 100 % (total plant death), and total chlorophyll content were recorded at 1, 2, 3, 6, 7, 10, 20 and 34 days after glyphosate application (DAA). Shikimate dehydrogenase and α-esterase isoenzyme assays were performed at 8, 24, 48, 72 and 144 h after application (HAA). Intoxication symptoms observed at 6 DAA ranged from 5 % at the lowest glyphosate dosage (1,440 g a. e. ha−1) to ~30 % at the highest dosage (4,320 g a. e. ha−1) across all cultivars. IACSP94-4004 and IACSP93-3046 were determined to be the most glyphosate-tolerant cultivars. Reduction in chlorophyll content was observed with high dosages of glyphosate at 6 DAA. The shikimate dehydrogenase banding pattern was generally conserved among cultivars and treatments, with the exception of the IACSP94-4004 cultivar, which showed alterations in the banding pattern of α-esterase after glyphosate application.

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Acknowledgments

The present research was financed by Fundação de Amparo a Pesquisa do Estado de São Paulo (FAPESP 2010/09016-9) and IAC (Instituto Agronômico de Campinas). R.C. Adriano receive Master fellowship from CAPES.

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Authors and Affiliations

  1. IAC/Centro de Cana-de-Açúcar, C.P. 206, CEP, Ribeirão Preto, SP, 14001-970, Brazil

    Rodrigo Cabral Adriano, Carlos Alberto Mathias Azania, Luciana Rossini Pinto & Andréa Aparecida de Pádua Mathias Azania

  2. Depto. Ciências Exatas, Universidade Estadual Paulista, Campus Jaboticabal, Via de Acesso Prof. Paulo Donato Castellane s/n, Jaboticabal, SP, 14884-900, Brazil

    Dilermando Perecin

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  1. Rodrigo Cabral Adriano
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  2. Carlos Alberto Mathias Azania
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  3. Luciana Rossini Pinto
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Correspondence to Luciana Rossini Pinto.

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Adriano, R.C., Azania, C.A.M., Pinto, L.R. et al. Phenotypic and biochemical responses of sugarcane cultivars to glyphosate application. Sugar Tech 15, 127–135 (2013). https://doi.org/10.1007/s12355-013-0209-x

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