Abstract
Three strains of white-rot fungi were tested in solid state fermentation and submerged culture to obtain enzymes for dyes biotransformation. Both sugarcane bagasse and dyes induced laccase and manganese peroxidase biosynthesis but laccase seems to be the main enzyme related to the decolourisation profiles. A variable behavior of strains was observed depending on inducers, fermentation system and characteristics of the strains. Crude enzyme of Earliella scabrosa obtained in solid state fermentation showed higher decolourisation percentage of Navy FNB and Red FN-3G dyes than Trametes maxima (13) and Ganoderma zonatum (B-18). T. maxima exhibited the best decolourisation percentage in submerged cultures supplemented with Navy FNB, Red FN- 3G and yellow P-6GS dyes. Growing biomass of T. maxima could supply other enzymes and mediators for dyes transformation. Peculiar behaviour was observed with Ganoderma zonatum (B-18), it had a similar dyes biodegradation in both liquid and solid bed fermentation and there was not positive correlation between ligninolytic enzymes production and decolourisation pattern. The employment of crude enzymes produced in solid bed of bagasse could be an attractive option for biological removal of textile dyes.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Abadulla E, Tzanov T, Robra KH, Cavaco-Paulo A, Gübitz GM (2000) Decolorisation and detoxification of textile dyes with a laccase from Trametes hirsute. Appl. Environ. Microbiol. 66: 3357–3362.
Cha CJ, Doerge DR, Cernigha CE (2001) Biotransformation of the Malachite green by the fungus Cunninghamella elegans. Appl. Environ. Microbiol. 67: 4358–4360.
Conneely A, Smyth WF, McMullan G (2002) Study of the white-rot fungal degradation of selected phthalocyanine dyes by capillary electrophoresis and liquid chromatography. Anal. Chim. Acta. 451: 259–270.
Eichlenová I, Homolka L, Lisá L, Nerud F (2005) Orange G and remazol brilliant blue R, decolourisation by white-rot fungi Dichomitus squalens, Ischroderma resinosum and Pleurotus colyptratus. Chemosphere 60: 398–404.
Gandolfi C, Obici L, Giatti M, Peralta R (2004) Decolourisation of synthetic dyes by solid state cultures of Lentinula (Lentinus) edodes producing manganese peroxidase as the main ligninolytic enzyme. Bioresource 94: 107–112.
Guerra G, Ramos-Leal M, Casado G, Argüelles J, Sánchez Ma I, Manzano AM, T Guzmán (2006) Cellulase production with sugarcane straw by Trichoderma citrinoviride on solid bed. Sugar Tech 8(1):30–35.
Hofrichter M, Vares T, Kalsi M, Galkin S, Scheibner K, Fritsche W, Hatakka A (1999) Production of Manganese Peroxidase and organic acids and mineralization of 14C-Labelled Lignin (14C-DHP) during solid state fermentation of wheat straw with the White-rot Fungus Nematoloma frowardii. Appl. Environ. Microbiol. 65: 1864–1870
Kahraman S, Gurdal IH (2002) Effect of synthetic and natural culture media on laccase production by white-rot fungi. Biores. Technol. 82: 215–217.
Kirby N, Marchant R, McMullan G (2000) Decolourisation of synthetic textile dyes by Phlebia tremellosa. FEMS Microbiol. Lett. 188: 93–96.
Kirst G., Zilly A, Giatti C, Peralta RM (2004) Decolourisation of industrial dyes by solid-state cultures of Pleurotus pulmonarius. Process Biochemistry 39: 855–859.
Kuan IC, Tien M (1993) Glyoxylate-suported reactions catalyzed by Mn peroxidase of Phanerochaete chrysosporium in the absence of added hydrogen peroxide. Arch. Biochem. Biophys. 302: 447–454.
Levin L, Papinutti L, Forchiassin F (2004) Evaluation of Argentinean white-rot fungi for their ability to produce lignin-modifying enzymes and decololourize industrial dyes. Biores. Technol. 94: 169–176.
Manzano AM (2004) Decoloración de tintes industriales de tipo azoico por cepas autóctonas de basidiomicetos de la podredumbre blanca. MSc Thesis. Faculty of Biology, Havana University, Cuba. 60 p.
Martínez AT (2002) Molecular biology and structure- function of lignindegrading beme peroxidases. Enzyme Microbiol. Technol., 30: 425–444.
Muñoz C, Guillen F, Martinez AT, Martinez MJ (1997) Laccase isoenzymes of Pleurotus eryngii. Characterization, catalytic properties, and participation in activation of molecular oxygen and Mn2+ oxidation. Appl. Environ. Microbiol.63: 2166–2174.
Novotny C, Erbanová P, Cajthaml T, Rolhschild N, Dosoretz C, Sasek V (2000) Irpex lacteus, a white-rot fungus applicable to water and soil bioremediation. Appl. Microbiol. Biotechnol. 54:850–853.
Rodríguez E, Nuero O, Guillén F, Martínez AT, Martínez MJ (2004) Degradation of phenolic and non phenolic aromatic pollutans by tour Pleurotus specie: the role of laccase and versatile peroxidase. Soil Biology and Biotechnol. 36: 909–916.
Sasaki T, Kajino T, Li B, Sugiyama H, Takahashi H (2001) New pulp biobleaching system involving Manganese peroxidase immobilized in a silica support with controlled pore size. Appl. Environ. Microbiol. 67: 2208–2212.
Swamy J, Ramsay JA (1999) The evaluation of white-rot fungi in the decolouration of textile dyes. Enzyme and Microbial Technol., 24:130–137.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Guerra, G., Domínguez, O., Ramos-Leal, M. et al. Production of laccase and manganese peroxidase by white-rot fungi from sugarcane bagasse in solid bed: Use for dyes decolourisation. Sugar Tech 10, 260–264 (2008). https://doi.org/10.1007/s12355-008-0046-5
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12355-008-0046-5