Abstract
Induced mutations have contributed significantly to plant improvement and more than 2500 mutant varieties have been developed through mutation breeding. Many examples related to different vegetatively propagated species show that the mutation induction can be empowered by in vitro techniques. Sugarcane is one of the most important industrial crops, used as major source of sugar and recently to produce ethanol, an important renewable biofuel source. At BARC, our group has been engaged in employing in vitro culture techniques in the improvement of sugarcane. Different in vitro culture systems have been developed and radiation induced mutagenesis has been undertaken using embryogenic callus cultures of cv. CoC-671 followed by the in vitro selection and field evaluation of the selected lines. Results on radiation-induced mutagenesis combined with in vitro selection have been reviewed. Also the novel approaches of overcoming intrasomatic competition during in vitro mutagenesis and use of using ion beam irradiation have been discussed.
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Patade, V.Y., Suprasanna, P. Radiation induced in vitro mutagenesis for sugarcane improvement. Sugar Tech 10, 14–19 (2008). https://doi.org/10.1007/s12355-008-0002-4
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DOI: https://doi.org/10.1007/s12355-008-0002-4