Abstract
Leucine-rich repeat receptor-like kinases (LRR-RLKs) represent the largest subgroup of receptor-like kinases (RLKs) in plants. While some LRR-RLK members play a role in regulating various plant growth processes related to morphogenesis, disease resistance, and stress response, the functions of most LRR-RLK genes remain unclear. In this study, we identified 397 LRR-RLK genes from the genome of Camellia sinensis and categorized them into 16 subfamilies. Approximately 62% of CsLRR-RLK genes are situated in regions resulting from segmental duplications, suggesting that the expansion of CsLRR-RLK genes is due to segmental duplications. Analysis of gene expression patterns revealed differential expression of CsLRR-RLK genes across different tissues and in response to stress. Furthermore, we demonstrated that CssEMS1 localizes to the cell membrane and can complement Arabidopsis ems1 mutant. This study is the initial in-depth evolutionary examination of LRR-RLKs in tea and provides a basis for future investigations into their functionality.
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Acknowledgements
This work was supported by the grants from General Research Projects of Zhejiang Provincial Department of Education (Y202351039), Huzhou Science and Technology Plan Project (2023GZ44), and Research Program of Huzhou College (2023HXKM09)
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ZZ designed the research. ZZ and FY wrote the manuscript. FY, KH, TL and ZM performed the identification of LRR-RLKs, protein structure, evolution analyses, and expression analyses. All authors read and approved the final manuscript.
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Table S1. Amino acid sequences of tea LRR-RLKs discovered in the current investigation. (TXT 349 KB)
12298_2024_1458_MOESM3_ESM.xlsx
Table S3. The comprehensive data on LRR-RLKs associated with tea as revealed in this current investigation. (XLSX 32 KB)
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Figure S1. Phylogenetic tree of LRR-RLK genes created using LRR-RLK genes sourced from Arabidopsis, rice, and tea. (PDF 878 KB)
Figure S2. The classification of groups for tea, rice and Arabidopsis LRR-RLK proteins. (PDF 150 KB)
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Figure S3. The conserved motifs identified in tea LRR-RLK proteins. The ten anticipated conserved structural domains are denoted by boxes of various colors (PDF 2416 KB)
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Zhang, Z., Ye, F., Hu, K. et al. New insights into evolution and functional diversification of Camellia sinensis LRR-RLKs. Physiol Mol Biol Plants 30, 851–866 (2024). https://doi.org/10.1007/s12298-024-01458-1
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DOI: https://doi.org/10.1007/s12298-024-01458-1