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Effect of thermospermine on expression profiling of different gene using massive analysis of cDNA ends (MACE) and vascular maintenance in Arabidopsis

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Abstract

Arabidopsis thaliana polyamine oxidase 5 gene (AtPAO5) functions as a thermospermine (T-Spm) oxidase. Aerial growth of its knock-out mutant (Atpao5-2) was significantly repressed by low dose(s) of T-Spm but not by other polyamines. To figure out the underlying mechanism, massive analysis of 3′-cDNA ends was performed. Low dose of T-Spm treatment modulates more than two fold expression 1,398 genes in WT compared to 3186 genes in Atpao5-2. Cell wall, lipid and secondary metabolisms were dramatically affected in low dose T-Spm-treated Atpao5-2, in comparison to other pathways such as TCA cycle-, amino acid- metabolisms and photosynthesis. The cell wall pectin metabolism, cell wall proteins and degradation process were highly modulated. Intriguingly Fe-deficiency responsive genes and drought stress-induced genes were also up-regulated, suggesting the importance of thermospermi′ne flux on regulation of gene network. Histological observation showed that the vascular system of the joint part between stem and leaves was structurally dissociated, indicating its involvement in vascular maintenance. Endogenous increase in T-Spm and reduction in H2O2 contents were found in mutant grown in T-Spm containing media. The results indicate that T-Spm homeostasis by a fine tuned balance of its synthesis and catabolism is important for maintaining gene regulation network and the vascular system in plants.

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Acknowledgements

Dr. Simit Patel is acknowledged for critically reading the manuscript. This work was supported by the Ministry of Education, Culture, Sports, Science and Technology of Japan (MEXT) to TK (26·04081, 15K14705) and Bangladesh Agricultural University Research System (BAURES) to GHMS (2017/262/BAU).

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Author notes

  1. G.H.M. Sagor and Stefan Simm these authors contributed equally to this work.

Authors and Affiliations

  1. Plant Molecular Genetics Laboratory, Department of Genetics & Plant Breeding, Bangladesh Agricultural University, Mymensingh, 2202, Bangladesh

    G. H. M. Sagor

  2. Graduate School of Life Sciences, Tohoku University, 2-1-1 Katahira, Aoba, Sendai, Miyagi, 980-8577, Japan

    Dong Wook Kim & Tomonobu Kusano

  3. Department of Biosciences, Molecular Cell Biology of Plants, Goethe University, Frankfurt am Main, Germany

    Stefan Simm

  4. Faculty of Pharmaceutical Sciences, Josai University, Sakado, Saitama, 370-0290, Japan

    Masaru Niitsu

  5. Senckenberg Biodiversity and Climate Research Center, Georg-Voigt-Str. 14-16, 60325, Frankfurt am Main, Germany

    Thomas Berberich

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  1. G. H. M. Sagor
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GHMS, SS, KDW, Performing lab experiment, collection and analysis of data. MN synthesized uncommon chemicals, GHMS, TK, TB: Design, formulation, supervision of experiment and writing of manuscript.

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Correspondence to G. H. M. Sagor.

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Sagor, G.H.M., Simm, S., Kim, D.W. et al. Effect of thermospermine on expression profiling of different gene using massive analysis of cDNA ends (MACE) and vascular maintenance in Arabidopsis. Physiol Mol Biol Plants 27, 577–586 (2021). https://doi.org/10.1007/s12298-021-00967-7

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  • DOI: https://doi.org/10.1007/s12298-021-00967-7

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