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Exogenous thermospermine has an activity to induce a subset of the defense genes and restrict cucumber mosaic virus multiplication in Arabidopsis thaliana

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Abstract

We previously proposed the defensive role of a signal pathway triggered by the polyamine spermine (Spm) in the reaction against avirulent viral pathogens in Nicotiana tabacum and Arabidopsis thaliana. In this study, we showed that thermospermine (T-Spm), an isomer of Spm, is also active in inducing the expression of the genes involved in the Spm-signal pathway at a similar dose as Spm. Furthermore, we found that T-Spm enhances the expression of a subset of pathogenesis-related genes whose expression is induced during cucumber mosaic virus (CMV)-triggered hypersensitive response. In consistent with the above observation, we further showed that exogenous T-Spm can repress CMV multiplication with same efficiency as Spm.

Key message

Polyamine thermospermine, an isomer of spermine, is able to induce a subset of hypersensitive response-related defense genes and can suppress cucumber mosaic virus multiplication in Arabidopsis thaliana.

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Acknowledgments

This work was supported in part by Grant-in-Aids from the Japan Society for the Promotion of Science (JSPS) to TK (21380063) and YT (21780087), and by the grant from The Saito Gratitude Foundation (to GHMS) and by the research funding programme “LOEWE-Landes-Offensive zur Entwicklung Wissenschaftlich-ökonomischer Exzellenz” of Hesse’s Ministry of Higher Education, Research, and the Arts (to TB). GHMS is a recipient of MEXT scholarship.

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Authors and Affiliations

  1. Graduate School of Life Sciences, Tohoku University, 2-1-1 Katahira, Aoba, Sendai, Miyagi, 980-8577, Japan

    G. H. M. Sagor, Yoshihiro Takahashi & Tomonobu Kusano

  2. Graduate School of Agricultural Sciences, Tohoku University, 1-1 Tsutsumidori-Amamiyamachi, Aoba, Sendai, Miyagi, 981-8555, Japan

    Hideki Takahashi

  3. Faculty of Pharmaceutical Sciences, Josai University, Sakado, Saitama, 350-0295, Japan

    Masaru Niitsu

  4. Biodiversity and Climate Research Center (BiK-F), BioCampus-Westend, 60323, Frankfurt am Main, Germany

    Thomas Berberich

Authors
  1. G. H. M. Sagor
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  2. Hideki Takahashi
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  3. Masaru Niitsu
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  4. Yoshihiro Takahashi
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  5. Thomas Berberich
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  6. Tomonobu Kusano
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Corresponding author

Correspondence to Tomonobu Kusano.

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Communicated by H. Ebinuma.

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Sagor, G.H.M., Takahashi, H., Niitsu, M. et al. Exogenous thermospermine has an activity to induce a subset of the defense genes and restrict cucumber mosaic virus multiplication in Arabidopsis thaliana . Plant Cell Rep 31, 1227–1232 (2012). https://doi.org/10.1007/s00299-012-1243-y

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  • DOI: https://doi.org/10.1007/s00299-012-1243-y

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