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Alteration of media enables efficient in vitro cloning of mature Elaeocarpus serratus L. (Ceylon olive): a commercially important fruit tree

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Abstract

Elaeocarpus serratus is a fruit tree able to propagate through conventional vegetative means to a limited extent restricts its wide cultivation by the farmers. In the present report, we have developed an efficient in vitro propagation protocol using mature nodal explants from a 17-year-old tree for the first time with 6.6 shoots/culture. Explants cultured on agar (0.8%) gelled standard Murashige and Skoog (MS) medium, ½ MS, ¾ MS, White’s, Gamborg’s B5 or woody plant medium (WPM) supplemented with 2.5 µM benzyl adenine (BA) and 0.1 µM α-naphthalene acetic acid (NAA) showed the superiority of ½ MS medium in terms of explant response and number shoots (6.6). Further optimization of ½ MS medium by altering nutrient elements (macros, micros, vitamins and Fe EDTA) were undertaken, and MS medium composed of half-strength major salts, original strength of minor salts and vitamins were supplemented with BA (2.5 µM) and NAA (0.1 µM), produced enhanced axillary bud proliferation (8.88/explant) and shoot elongation (3.83 cm). Reculturing of original explant on this medium after IV passages produced more than 16 healthy shoots per culture which attained a length of 4.13 cm. Microshoots raised through this way were rooted (86.11%) ex vitro by pulse treatment with 2 mM indole-3-butyric acid (IBA) for 5 min followed by planting in nursery pots containing a 1:1:1 (v/v/v) mix of sand, soil, and farmyard manure. The hardened plants were successfully planted in the fruit tree garden of the Department. Genetic fidelity of micropropagated and mother plants were tested using random amplified polymorphic DNA (RAPD) and inter simple sequence repeat (ISSR) markers which showed a high degree of monomorphism thus supported morphological uniformity of micropropagated plants.

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Acknowledgements

The authors are grateful to Dr. Suhara Beevy S, Professor and Head, Department of Botany for providing the facilities and Kerala State Council for Science Technology and Environment (KSCSTE), Government of Kerala, Thiruvananthapuram, India, for the financial assistance (P.1409/2014/KSCSTE) through Junior Research Fellowship.

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  1. Department of Botany, University of Kerala, Kariavattom, Trivandrum, 695581, India

    R. Raji & E. A. Siril

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RR and EAS designed the experiments. RR performed the experiments and data collection. EAS and RR analyzed data. RR prepared the first draft of the manuscript and EAS edited and communicated for publishing.

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Correspondence to E. A. Siril.

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Raji, R., Siril, E.A. Alteration of media enables efficient in vitro cloning of mature Elaeocarpus serratus L. (Ceylon olive): a commercially important fruit tree. Physiol Mol Biol Plants 27, 429–443 (2021). https://doi.org/10.1007/s12298-021-00955-x

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