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Genetic relationships and diversity among populations of Paris polyphylla assessed using SCoT and SRAP markers

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Abstract

The genetic diversity of 33 Paris polyphylla samples collected from the Dabie Mountains was analyzed using SCoT and SRAP molecular markers, revealing the genetic relationships among Paris polyphylla resources in the Dabie Mountains at the molecular level and providing a theoretical basis for genetic improvement and conservation. As a result, a total of 134 bands were amplified with 9 SCoT primers, the percentage of polymorphic bands was 100%, the average number of primers amplified was 14.89, the PIC value was 94.83% and the genetic similarity coefficient ranged from 0.463 to 0.896. Ten pairs of SRAP primer combinations amplified 135 bands, including 129 polymorphic bands, and the percentage of polymorphic bands was 95.56%. The average number of polymorphic bands obtained with each pair of SRAP primer combinations was 12.9, the PIC value was 93.91%, and the genetic similarity coefficient ranged from 0.533 to 0.904. This study showed that both SCoT and SRAP markers were suitable for the genetic diversity analysis of P. polyphylla, which belongs to a genus in which SRAP marker technology has not previously been applied, despite its application in a variety of other plants.

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Acknowledgements

Authors are thankful to the teachers and reviewers who give me valuable suggestions on this article.

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Authors and Affiliations

  1. School of Life Sciences, Anhui Agricultural University, Hefei, 230061, People’s Republic of China

    Xiaopei Zhao, Gaofen Zou, Jie Zhao, Linyi Hu, Yuefeng Lan & Jinling He

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  1. Xiaopei Zhao
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  2. Gaofen Zou
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  3. Jie Zhao
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  4. Linyi Hu
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  5. Yuefeng Lan
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  6. Jinling He
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Corresponding Author: Jinling He.

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Appendix

Appendix

See Figs. 5 and 6.

Fig. 5
figure 5

Amplification profiles of thirty-three P. polyphylla DNA samples using SCoT markers. Lanes: M = 5000 bp DNA Marker; 1–33:33 samples

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Fig. 6
figure 6

Amplification profiles of thirty-three P. polyphylla DNA samples using SRAP markers. Lanes: M = 5000 bp DNA Marker; 1–33:33 samples

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Zhao, X., Zou, G., Zhao, J. et al. Genetic relationships and diversity among populations of Paris polyphylla assessed using SCoT and SRAP markers. Physiol Mol Biol Plants 26, 1281–1293 (2020). https://doi.org/10.1007/s12298-020-00808-z

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