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Differential responses of Phaseolus vulgaris cultivars following mungbean yellow mosaic India virus infection

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Abstract

Phaseolus vulgaris, commonly known as French bean is a vital leguminous crop worldwide and India stood 1st rank in dry bean and 4th rank in green bean production worldwide (FAOSTAT 2017). However, this production is severely affected by Mungbean yellow mosaic India virus (MYMIV) infection. Hence it is very important to identify MYMIV tolerant P. vulgaris cultivars. MYMIV infection results in the production of reactive oxygen species and plant cells have evolved complex defense mechanisms at different levels to overcome the damage. Our study for the first time focused on the changes at the morphological and biochemical level, as well as on the relative quantification of MYMIV genes in nine cultivars of P. vulgaris after MYMIV infection. Highest growth and the highest accumulation of four antioxidants of cv. ‘Anupam’ after MYMIV infection, established that cv. ‘Anupam’ was less affected by MYMIV infection amongst all nine cultivars. Relative quantification studies also correlated well with these results. Additionally, there is a consistent level of photosynthetic pigments content in mock- and MYMIV-treated seedlings of cv. ‘Anupam’ over early infection period. Combining all the results we conclude that cv. ‘Anupam’ is a MYMIV tolerant cultivar.

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Abbreviations

YMD:

Yellow mosaic disease

MYMIV:

Mungbean yellow mosaic India virus

CP:

Coat protein

Rep:

Replication associated protein

TrAP:

Transcription activator protein

REn:

Replication enhancer protein

NSP:

Nuclear shuttle protein

MP:

Movement protein

RCR:

Rolling circle replication

H2O2 :

Hydrogen peroxide

ROS:

Reactive oxygen species

SOD:

Superoxide dismutase

CAT:

Catalase

RT-qPCR:

Real time quantitative polymerase chain reaction

ANOVA:

One-way analysis of variance

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Acknowledgements

JB acknowledges Department of Biotechnology, Govt. of India.

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The funding was provided by Department of Biotechnology, Government of India.

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  1. Nisha Patwa

    Present address: Horticultural Insects Research Laboratory, USDA-ARS, Application Technology Research Unit, 1680 Madison Ave., Wooster, OH, 44691, USA

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  1. Department of Biotechnology, Visva-Bharati, Siksha Bhavana, Santiniketan, West Bengal, 731235, India

    Nisha Patwa, Chitra Chatterjee & Jolly Basak

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  1. Nisha Patwa
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NP, CC and JB envisioned the study and participated in its design and coordination. NP and CC carried out experimental work, statistical analysis of the data and wrote the final manuscript.

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Correspondence to Jolly Basak.

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Patwa, N., Chatterjee, C. & Basak, J. Differential responses of Phaseolus vulgaris cultivars following mungbean yellow mosaic India virus infection. Physiol Mol Biol Plants 26, 817–828 (2020). https://doi.org/10.1007/s12298-019-00741-w

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