Abstract
The present study was aimed at understanding the role of different hosts in ammonium transporter1;2 expressions and glutamine synthetase(GS) activity and their effects on the growth parameters in the sandal. Sandal plant associated with leguminous host expressed better growth parameters. GS activity of leguminous hosts alone and in host associated sandals was analyzed using GS transferase assay. Highest GS activity was expressed in Mimosa pudica—sandal association compared to other leguminous and non-leguminous host associations. The association of N2 fixing host with sandal enhanced C and N levels in order to maintain the C/N value. The role of ammonium transporters in N nutrition of sandal-host association was elucidated by cloning AMT1;2 from the leaves, haustoria and roots of host associated sandal and quantifying the relative expression by the \( 2^{{ - \Delta \Delta {\text{C}}_{\text{T}} }} \) method. SaAMT1;2 was strongly up-regulated in leaves, roots and haustoria of leguminous host associated sandal compared to non-leguminous host associations. The relative increase in SaAMT1;2 expressions and up-regulated GS activity positively affected the growth parameters in sandal when associated with leguminous hosts.
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Abbreviations
- AMT:
-
Ammonium transporter
- BLAST:
-
Basic local alignment search tool
- CTAB:
-
Cetyltrimethyl ammonium bromide
- DTT:
-
Dithiothreitol
- EDTA:
-
Ethylenediamine tetra acetic acid
- GS:
-
Glutamine synthetase
- HATS:
-
High-affinity ammonium transporter system
- IUCN:
-
International Union for Conservation of Nature
- LATS:
-
Low-affinity ammonium transporter system
- PMSF:
-
Phenylmethylsulfonyl fluoride
- PVP:
-
Polyvinylpyrrolidone
- PVPP:
-
Polyvinylpolypyrrolidone
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The authors are thankful to the Director, Interuniversity Centre for Plant Biotechnology, Department of Botany, University of Calicut, Kerala for providing the facilities.
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Deepa, P., Yusuf, A. Influence of different host associations on glutamine synthetase activity and ammonium transporter in Santalum album L.. Physiol Mol Biol Plants 22, 331–340 (2016). https://doi.org/10.1007/s12298-016-0368-9
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DOI: https://doi.org/10.1007/s12298-016-0368-9