Abstract
An ideal micropropagation method of Polygonatum verticillatum has been developed using stem disc explants. Multiple shoots were initiated from stem disc explants on Murashige and Skoog (MS) medium fortified with different concentrations (0.25–10.0 mgl−1) and combinations of cytokinins (BAP, Kn and TDZ) along with (0.5–1.0 mgl−1) auxins (NAA/IBA/IAA). 1.0 mgl−1 BAP with 0.5 mgl−1 NAA was found to be the most effective in producing maximum number of shoots. Regular subculturing of these in vitro multiple shoots induced profuse growth of lateral roots in the same medium. Individual shoots were excised and rooted in vitro on half strength MS medium with 1.0 mgl−1 NAA. Regenerants were hardened in growth chamber with high humidity and showed a high rate of survival.
Similar content being viewed by others
References
Chaumont JP (1979) Antifungal activity of eight flowering plant against pathogenic fungi. Bull Bot Soc Fr 126:537–541
Islam MT, Keller ERJ, Philibert D (2008) Effect of growth regulators on in vitro propagation and Tuberisation of four Dioscorea species. Plant Tissue Cult Biotechnol 18:25–35
Kukreja AK, Dhawan OP, Mathur AK, Ahuja PS, Mandal S (1991) Screening and evaluation of aagronomically useful somaclonal variations in Japanese mint (Mentha arvensis L.). Euphytica 53:183–191
Lal N, Ahuja PS (1993) Assessment of liquid culture procedure for in vitro propagation of Rheum emodi. Plant Cell Tissue Organ Cult 34:223–226
Mathur J, Ahuja PS, Mathur A, Kukreja AK, Shah NC (1987) In vitro propagation of Valeriana wallichi. Planta Medica 39:187,277,1–187,277,3
Mercier H, Vieira CCJ, Figueiredo-Ribeiro RCL (1992) Tissue culture and plant propagation of Gomphrena officinalis—a Brazilian medicinal plant. Plant Cell Tissue Organ Cult 28:249–225
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497
Murthy KSR, Kondamudi R, Vijayalakshmi V (2010) Micropropagation of an endangered medicinal plant Ceropegia spiralis L. J Agr Tech 6(1):179–191
Pasqua G, Monacelli B, Giuliano M (1993) Influence of two different growth regulators on the adventitious root origin in vitro of Vismia guianensis regenerated plantlets. Ann Bot Roma 51:117–125
Pattnaik S, Chand PK (1996) In vitro propagation of the medicinal herbs Ocimum americanum L. syn. Ocimum canum Sims. (hoary basil) and Ocimum sanctum L. (holy basil). Plant Cell Rep 15:846–850
Purohit SD, Dave A, Gotam K (1994) Micropropagation of safed musli (Chlorophytum borivilianum), a rare Indian medicinal herb. Plant Cell Tissue Organ Cult 39:93–96
Sudha G, Seeni S (1994) In vitro multiplication and establishment of Adhatoda beddomei C.B Clark, a rare medicinal plant. Plant Cell Rep 13:203–207
Varier PS (1994) Indian medicinal plants: a compendium of 500 species. Madras, Orient Longman. 336–338.Wealth of India- Raw Materials, CSIR, New Delhi, 1976, 9: 365
Yamaner O, Erdag B (2008) Direct shoot formation and microtuberization from aseptic seedlings of Cyclamen mirabile Hildebr. Biotechnol 7(2):328–332
Acknowledgements
We are thankful to University Grants Commission New Delhi for financial support to carry out the present work.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Bisht, S., Bisht, N.S. & Bhandari, S. In vitro micropropagation in Polygonatum verticillatum (L.) All. an important threatened medicinal herb of Northern India. Physiol Mol Biol Plants 18, 89–93 (2012). https://doi.org/10.1007/s12298-011-0091-5
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12298-011-0091-5