Skip to main content
SpringerLink
Log in

Association of Low Serum Iron with Alpha Globin Gene Deletions and High Level of HbF with Xmn-1 Polymorphism in Sickle Cell Traits

  • Original Article
  • Published:
Indian Journal of Clinical Biochemistry Aims and scope Submit manuscript

Abstract

Usually sickle cell traits are asymptomatic but co-existence of various factrors may alter the clinical as well as biochemical levels. In India sickle cell traits are neglected condition. Here we are presenting the alpha deletion in association with low serum iron and increased HbF level with Xmn-1 carriers in sickle cell traits. Sickle traits with alpha deletions had significantly low level of serum iron (P-value <0.05) with low level of reticulocytes and red cell indices while Xmn-1 polymorphism associated with increased HbF level. Study concludes low serum iron associated with alpha deletions and high level of HbF associated with Xmn-1 polymorphism in sickle cell traits.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  1. Eichner ER. Sickle cell trait. J Sport Rehabil. 2007;16(3):197–203.

    PubMed  Google Scholar 

  2. Tsaras G, Owusu-Ansah A, Boateng FO, Amoateng-Adjepong Y. Complications associated with sickle cell trait: a brief narrative review. Am J Med. 2009;122(6):507–12.

    Article  PubMed  Google Scholar 

  3. Kanavakis E, Wainscoat JS, Wood WG, Weatherall DJ, Cao A, Furbetta M, et al. The intraction of alpha thalassemia with hetrozygus beta thalassemia. Br J Haematol. 1982;52(3):465–73.

    Article  PubMed  CAS  Google Scholar 

  4. Wainscoat JS, Kanavakis E, Wood WG, Letsky EA, Huehns ER, Marsh GW, Higgs DR, et al. Thalassemia intermedia in Cyprus: the interaction of alpha and beta thalassemia. Br J Haematol. 1983;53(3):411–6.

    Article  PubMed  CAS  Google Scholar 

  5. Gilman JG, Huisman THJ. DNA sequence variation associated with elevated fetal Gg globin production. Blood. 1985;66:783–7.

    PubMed  CAS  Google Scholar 

  6. Dedoussis GV, Mandilara GD, Boussiv M, Loutradis A. HbF production in b-thalassaemia heterozygotes for the IVSII-1 G-A β0-globin mutation. Implication of the haplotype and the Gg-158 C-T mutation on the HbF level. Am J Hematol. 2000;64(3):151–5.

    Article  PubMed  CAS  Google Scholar 

  7. Waterfall CM, Benjamin DC. Single tube genotyping of sickle cell anaemia using PCR-based SNP analysis. Nucleic Acids Res. 2001;29(23):e119.

    Article  PubMed  CAS  Google Scholar 

  8. Baysal E, Huisman THJ. Detection of common deletional alpha thalassemia-2 determinants by GAP-PCR. Am J Hematol. 1994;46:208–13.

    Article  PubMed  CAS  Google Scholar 

  9. Smetanina NS, Huisman TH. Detection of alpha-thalassemia-2 (−3.7 kb) and its corresponding triplication ααα (anti-3.7 kb) by PCR: an improved technical change. Am J Hematol. 1996;53(3):202–3.

    Article  PubMed  CAS  Google Scholar 

  10. Shaji RV, Eunice SE, Baidya S, Srivastava A, Chandy M. Determination of the breakpoint and molecular diagnosis of a common alpha-thalassaemia-1 deletion in the Indian population. Br J Haematol. 2003;123(5):942–7.

    Article  PubMed  CAS  Google Scholar 

  11. Chang JG, Lee LS, Lin CP, Chen PH, Chen CP. Rapid diagnosis of alpha–thalassemia-1 of Southeast Asia type and hydrops fetailis by polymerase chain reaction. Blood. 1991;78:853–4.

    PubMed  CAS  Google Scholar 

  12. Sutton M, Bouhassira EE, Nagel RL. Polymerase chain reaction amplification applied to the determination of beta like globin gene cluster haplotypes. Am J Hematol. 1989;32:66–9.

    Article  PubMed  CAS  Google Scholar 

  13. Morris CR. Mechanisms of vasculopathy in sickle cell disease and thalassemia. Hematol Am Soc Hematol Educ Progr. 2008;177–185.

  14. Stuart MJ, Nagel RL. Sickle-cell disease. Lancet. 2004;364:1343–60.

    Article  PubMed  Google Scholar 

  15. Koduri PR. Iron in sickle cell disease: a review why less is better. Am J Hematol. 2003;73:59–63.

    Article  PubMed  CAS  Google Scholar 

  16. Raffaella M, Paola T, Matteo P, Alberto P. Iron metabolism in thalassemia and sickle cell disease. Medit J Hemat Infect Dis. 2009;1(1): Open Journal System.

  17. Julius B, Louis JF. Sickle cell disease: with special regard to its non anemic variety. Arch Surg. 1943;47(6):553–63.

    Article  Google Scholar 

  18. Nava MP, Ibarra B, Magaña MT, de la Luz Chávez M, Perea FJ. Prevalence of −α3.7 and αααanti3.7 alleles in sickle cell trait and β-thalassemia patients in Mexico. Blood Cells Mol Dis. 2006;36(2):2255–8.

    Article  Google Scholar 

  19. Higgs DR, Aldridge BE, Lamb J, Clegg JB, Weatherall DJ, Hayes RJ, Grandison Y, et al. The interaction of alpha thalassemia and homozygous sickle cell disease. N Eng J Med N Engl J Med. 1982;306(24):1441–6.

    Article  CAS  Google Scholar 

  20. Embury SH, Dozy AM, Miller J, Davis JR, Kleman KM, Preisler H, et al. Concurrent sickle cell anemia and alpha thalassemia. Effect on severity. N Engl J Med. 1982;306(5):270–4.

    Article  PubMed  CAS  Google Scholar 

  21. de Ceulaer K, Higgs DR, Weatherall DJ, Hayes RJ, Serjeant BE, Serjeant GR. Alpha thalassemia reduces the hemolytic rate in homozygous sickle cell disease. N Engl J Med. 1983;309(3):189–90.

    Article  PubMed  Google Scholar 

  22. Garner C, Tatu T, Reittie JE, Littlewood T, Darley J, Cervino S, et al. Genetic influences on F cells and other hematologic variables: a twin heritability study. Blood. 2000;95:342–6.

    PubMed  CAS  Google Scholar 

  23. Serjeant GR. Sickle cell- β thalassemia. In: Serjeant GR, editor. Sickle Cell Disease. 3rd ed. Oxford: Oxford University Press; 2001.

  24. Panigrahi I, Agarwal S, Gupta T, Singhal P, Pradhan M. Hemoglobin E-beta thalassemia: factors affecting phenotype. Indian Pediatr. 2005;42:357–62.

    PubMed  CAS  Google Scholar 

  25. Shimmoto MM, Vicari P, Fernandes AC, Guimaraes GS, Figueiredo MS. XmnI polymorphism is associated with fetal hemoglobin levels in hypoplastic syndromes. Sao Paulo Med J. 2006;124(2):110–1.

    Article  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Hematology, All India Institute of Medical Sciences, Ansari Nagar, New Delhi, 110 029, India

    S. Pandey, A. Suhail, S. Rahul, K. Ravi, Sw. Pandey, T. Seth & R. Saxena

  2. Department of Environmental Biology, APS University Rewa, Rewa, India

    R. M. Mishra

Authors
  1. S. Pandey
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. R. M. Mishra
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. A. Suhail
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. S. Rahul
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. K. Ravi
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Sw. Pandey
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. T. Seth
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. R. Saxena
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to R. Saxena.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Pandey, S., Mishra, R.M., Suhail, A. et al. Association of Low Serum Iron with Alpha Globin Gene Deletions and High Level of HbF with Xmn-1 Polymorphism in Sickle Cell Traits. Ind J Clin Biochem 27, 270–273 (2012). https://doi.org/10.1007/s12291-011-0170-y

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12291-011-0170-y

Keywords

Search

Navigation