Abstract
Cardiometabolic syndrome is one of the major public health issues of this century which describes a cluster of clinical characteristics. Seventy two patients with coronary artery disease (CAD) and cardiometabolic syndrome and forty healthy age and sex matched normal controls were selected for this study. Detailed clinical epidemiological and anthropometric characteristics were noted. Lipid profile and Cytokinesis-block micronuclei (CBMN) assay using cytochalasin B were carried out in all the subjects. Serum total cholesterol, triglyceride and LDL-cholesterol was significantly higher and HDL cholesterol was significantly lower in patients compared to their normal counter-parts (P<0.05). CBMN frequency of the patients was significantly higher at all ages compared to their normal counter parts (P<0.05). Various risk factors like diabetes, hypertension, dyslipidemia, abdominal obesity, smoking and alcoholism were found influenced the CBMN frequency; but the changes were not significant. From this study it can be concluded that DNA damage was found to be higher in patients with cardiometabolic syndrome which may be attributed to the generation of free radicals associated with alcohol consumption, tobacco use, dyslipidemia and glucose intolerance and the accumulation of free radicals with increase in age.
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Grundy SM, Cleeman JI, Daniels SR, Donato KA, Eckel RH, Franklin BA, et al. Diagnosis and management of the metabolic syndrome. An American Heart Association/National Heart, Lung, and Blood Institute Scientific Statement. Circulation 2005; 112:2735–2752.
Govindarajan G, Whaley-Connell A, Mugo M, Stump C, Sowers JR. The cardiometabolic syndrome as a cardiovascular risk factor. Am J Med Sci 2005; 330(6): 311–318
Pandey S, Baral N, Majhi S, Acharya P, Karki P, Shrestha S, et al. Prevalence of the metabolic syndrome in acute myocardial infarction and its impact on hospital outcomes. Int J Diab Dev Ctries 2009; 29: 52–55
Frisard MI, Rood JC, Fang X, Su J, Welsh DA, Jazwinski SM, Ravussin E Metabolic syndrome and risk factors for cardiovascular disease: are nonagenarians protected? Age (Dordr) 2009; 31(1): 67–75.
Eckel RH, Grundy SM, Zimmet PZ. The metabolic syndrome. Lancet 2005; 365:1415–1428.
Andreassi MG. Metabolic syndrome, diabetes and atherosclerosis: influence of gene-environment interaction. Mutat Res 2009; 667(1–2):35–43.
Botto N, Mssetti S, Petrozzi L. Elevated levels of oxidative DNA damage in patients with coronary artery disease. Coron Artery Dis 2002; 13:269–274.
Andreassi MG. Coronary atherosclerosis and somatic mutations: an overview of the contributive factors for oxidative DNA damage. Mutat Res 2003; 543: 67–86.
Demirbag R, Yilmaz R, Gur M, Celik H, Guzel S, Selek S et al. DNA damage in metabolic syndrome and its association with antioxidative and oxidative measurements. Int J Clin Pract 2006; 60: 1187–1193.
Rifai N, Bachorik PS, Albers JJ. Lipids,lipoprotein and apolipoprotein. In Burtis CA, Ashwood R., editors. Tietz textbook of clinical chemistry 3rd ed. Philadelphia. W.B. Saunders company. 1999: 806–861.
Mc Gowan MW, Artiss JD, Standbergh DR, Zark B. A peroxidase coupled method for the colorimeteric determination of serum triglycerides. Clin Chem 1983; 29(3): 538–542.
Sugiuchi H, Uji Y, Okabe H, Irie T, Uekama K, Kayahara N, et al. Direct measurement of High-Density Lipoprotein Cholesterol in serum with polyethylene glycol-modified enzymes and sulphated alpha-cyclodextrin. Clin Chem 1995; 41:717–723.
Armstrong V, Seidel D. Evaluation of a commercial kit for the determination of LDL-cholesterol in serum based on precipitation of LDL with dextran sulphate. Arztl Lab 1985; 31: 325–330.
Moorhead PS, Nowell PC, Mellman WJ, Battips DM, Hungerford DA. Chromosome preparations of leukocytes cultured from human peripheral blood. Exp Cell Res 1960; 20: 613–616.
Fenech M. The cytokinesis-block micronucleus technique and its application to genotoxicity studies in human populations. Environmental Health Perspectives 1993; 101(3):101–107.
Ford ES, Giles WH, Dietz WH. Prevalence of the metabolic syndrome in U.S. adults. JAMA 2002; 287: 356–359.
Deepa M, Farooq S, Datta M, Deepa R, Mohan V. Prevalence of metabolic syndrome using WHO, ATP III, and IDF definitions in Asian Indians: the Chennai Urban Rural Epidemiology Study (CURES-34). Diabetes Metab Res Rev 2007; 23: 127–134.
Abbott RD, Curb JD, Rodriguez BL, Masaki KH, Yano K, Schatz IJ, et al. Age-related changes in risk factor effects on the incidence of coronary heart disease. Ann Epidemiol 2002; 12: 173–181.
Ghosh J, Mishra T K, Rao Y N, Aggarwal S K. Oxidised LDL, HDL cholesterol, LDL cholesterol levels in patients of coronary artery disease. Ind J Clin Biochem 2006; 21: 181–184.
Lahdenpera S, Syvanne M, Kahri J, Taskinen MR. Regulation of low-density lipoprotein particle size distribution in NIDDM and coronary disease: importance of serum triglycerides. Diabetologia 1996; 39(4):453–461.
Ishizaka N, Ishizaka Y, Toda E, Hashimoto H, Nagai R, Yamakado M. Association between cigarette smoking, metabolic syndrome, and carotid arteriosclerosis in Japanese individuals. Atherosclerosis 2005; 181: 381–388.
Nakanishi N, Takatorige T, Suzuki K. Cigarette smoking and the risk of the metabolic syndrome in middle-aged Japanese male office workers. Ind Health 2005; 43: 295–301.
Ramachandran A, Snehalatha C, Satyavani K, Weiss R, Dziura J, Burgert TS. Metabolic syndrome in urban Asian Indian adults: a population study using modified ATP III criteria. Diabetes Res Clin Pract 2003; 60: 199–204.
Balkau B, Picard P, Vol S, Fezeu L and Eschwège E. Consequences of Change in Waist Circumference.Diabetes Care 2007; 30(7): 1901–1903.
Wang Y, Rimm EB, Stampfer MJ, Willett WC, Hu FB. Comparison of abdominal adiposity and overall obesity in predicing risk of Type 2 diabetes among man. Am J Clin Nutr 2005;81:555–563.
Haffner SM, Stern MP, Hazuda HP, Mitchell BD, Patterson JK. Cardiovascular risk factors in confirmed prediabetic individuals. Does the clock for coronary heart disease start ticking before the onset of clinical diabetes? JAMA 1990; 263: 2893–2898.
McPhillips JB, Barrett-Connor E, Wingard DL. Cardiovascular disease risk factors prior to the diagnosis of impaired glucose tolerance and non-insulin-dependent diabetes mellitus in a community of older adults. Am J Epidemiol 1990; 131: 443–453.
Mykkanen L, Kuusisto J, Pyorala K, Laakso M. Cardiovascular disease risk factors as predictors of type 2 (non-insulin-dependent) diabetes mellitus in early subjects. Diabetologia 1993; 36: 553–559.
Zimmet PZ, Alberti KGMM. The changing face of macrovascular disease in non-insulin-dependent diabetes mellitus: an epidemic in progress. Lancet 1997;350(1):1–4.
Mahadik SR, Deo SS, Mehtalia SD. Increased prevalence of metabolic syndrome in non-obese Asian Indian-an urban-rural comparison. Metab Syndr Relat Disord 2007; 5(2): 142–152
Fan AZ, Russell M, Dorn J, Freudenheim JL, Nochajski T, Hovey K, et al. Lifetime alcohol drinking pattern is related to the prevalence of metabolic syndrome. The Western New York Health Study (WNYHS). Eur J Epidemiol 2006; 21:129–138.
Wei-ju L, Hao X, Kai S, Xiao-dong S, Yi-bo W, Yi-song Z, et al Cardiovascular risk and prevalence of metabolic syndrome by differing criteria. Chin Med J 2008; 121: 1532–1536.
Nishtha J, Imrana N, Jamal A. Evaluation of DNA damage and metabolic syndrome parameters in diabetic rabbits supplemented with antioxidants. Fundamental Clin Pharmacol 2009; 23: 197–205
Satoh M, Ishikawa Y, Takahashi Y, Itoh T, Minami Y, Nakamura M Association between oxidative DNA damage and telomere shortening in circulating endothelial progenitor cells obtained from metabolic syndrome patients with coronary artery disease. Atherosclerosis 2008; 198(2): 347–353.
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Supriya Simon, A., Dinesh Roy, D., Jayapal, V. et al. Biochemical and genetic studies on cardiometabolic syndrome. Indian J Clin Biochem 25, 164–168 (2010). https://doi.org/10.1007/s12291-010-0030-1
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DOI: https://doi.org/10.1007/s12291-010-0030-1