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Saxibacter everestensis gen. nov., sp. nov., A Novel Member of the Family Brevibacteriaceae, Isolated from the North Slope of Mount Everest

  • Evolutionary Microbiology and Systematics
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Abstract

We isolated and analyzed a novel, Gram-stain-positive, aerobic, rod-shaped, non-motile actinobacterium, designated as strain ZFBP1038T, from rock sampled on the north slope of Mount Everest. The growth requirements of this strain were 10–37 °C, pH 4–10, and 0–6% (w/v) NaCl. The sole respiratory quinone was MK-9, and the major fatty acids were anteiso-C15:0 and iso-C17:0. Peptidoglycan containing meso-diaminopimelic acid, ribose, and glucose were the major cell wall sugars, while polar lipids included diphosphatidyl glycerol, phosphatidyl glycerol, an unidentified phospholipid, and an unidentified glycolipid. A phylogenetic analysis based on 16S rRNA gene sequences showed that strain ZFBP1038T has the highest similarity with Spelaeicoccus albus DSM 26341 T (96.02%). ZFBP1038T formed a distinct monophyletic clade within the family Brevibacteriaceae and was distantly related to the genus Spelaeicoccus. The G + C content of strain ZFBP1038T was 63.65 mol% and the genome size was 4.05 Mb. Digital DNA–DNA hybridization, average nucleotide identity, and average amino acid identity values between the genomes of strain ZFBP1038T and representative reference strains were 19.3–25.2, 68.0–71.0, and 52.8–60.1%, respectively. Phylogenetic, phenotypic, and chemotaxonomic characteristics as well as comparative genome analyses suggested that strain ZFBP1038T represents a novel species of a new genus, for which the name Saxibacter gen. nov., sp. nov. was assigned with the type strain Saxibacter everestensis ZFBP1038T (= EE 014 T = GDMCC 1.3024 T = JCM 35335 T).

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Data availability

All genomic data from this study have been deposited in the NCBI Genbank (https://submit.ncbi.nlm.nih.gov/subs/genome/), with the registry number CP090958, JAJTWV000000000, JAJTWX000000000 and JAJTWW000000000.

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Acknowledgements

This work was supported by the National Key R and D Program of China (2019YFE0121100), and the National Natural Science Foundation of China (42071099, 42371156).

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  1. Mao Tian and Shiyu Wu contributed equally to this work.

Authors and Affiliations

  1. State Key Laboratory of Cryospheric Sciences, Northwest Institute of Eco-Environment and Resources, Chinese Academy of Sciences, Lanzhou, 730000, People’s Republic of China

    Mao Tian, Shiyu Wu, Binglin Zhang & Tuo Chen

  2. Key Laboratory of Extreme Environmental Microbial Resources and Engineering, Lanzhou, Gansu Province, 730000, People’s Republic of China

    Mao Tian, Shiyu Wu, Wei Zhang, Gaosen Zhang, Xue Yu, Yujie Wu, Puchao Jia, Binglin Zhang, Tuo Chen & Guangxiu Liu

  3. University of Chinese Academy of Sciences, Beijing, 100049, People’s Republic of China

    Mao Tian, Shiyu Wu, Xue Yu, Yujie Wu, Puchao Jia & Binglin Zhang

  4. Key Laboratory of Desert and Desertification, Northwest Institute of Eco-Environment and Resources, Chinese Academy of Sciences, Lanzhou, 730000, People’s Republic of China

    Wei Zhang, Gaosen Zhang, Xue Yu, Yujie Wu, Puchao Jia & Guangxiu Liu

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Contributions

M.T., W.Z., G.Z., Y.W., G.L., and T.C. conceived and designed the study. M.T., G.Z., A.B., Y.W., and X.Y. carried out the experiments. M.T., X.Y., and S.W. performed data analysis and figure drawing. M.T. and S.W. wrote the manuscript. W.Z., A.B., G.L., T.C., Y.W., and S.W. supervised this work and edited the manuscript. All authors read and approved the final draft.

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Correspondence to Wei Zhang.

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Tian, M., Wu, S., Zhang, W. et al. Saxibacter everestensis gen. nov., sp. nov., A Novel Member of the Family Brevibacteriaceae, Isolated from the North Slope of Mount Everest. J Microbiol. (2024). https://doi.org/10.1007/s12275-024-00108-1

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