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Prevalence of Indigenous Antibiotic-Resistant Salmonella Isolates and Their Application to Explore a Lytic Phage vB_SalS_KFSSM with an Intra-Broad Specificity

  • Virology
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Abstract

The consumption of fresh produce has led to increase in antibiotic-resistant (AR) Salmonella outbreaks. In this study, indigenous Salmonella was isolated from a total of two hundred-two samples including fresh produce and agricultural environmental samples in Korea. After biochemical confirmation using the Indole, Methyl Red, Voges-Proskauer, Citrate tests, presumable Salmonella isolates were identified by 16S rRNA sequencing. Identified Salmonella isolates were evaluated for antibiotic susceptibility against twenty-two antibiotics. The specificity and the efficiency of plating (EOP) of vB_SalS_KFSSM were evaluated against fifty-three bacterial strains. Twenty-five suspected Salmonella were isolated and confirmed by the positive result for methyl red and citrate, of which ten were identified as Salmonella spp. through 16S rRNA gene sequencing. Eight Salmonella isolates (4.0%, n = 8/202) were resistant to at least one antibiotic, among which five were multi-drug resistant. As a lytic phage against Salmonella spp. CMGS-1, vB_SalS_KFSSM was isolated from cow manure. The phage was observed as a tailed phage belonging to the class Caudoviricetes. It exhibited an intra-broad specificity against four indigenous AR Salmonella isolates, two indigenous Salmonella isolates, and five other Salmonella serotypes with great efficiencies (EOP ≥ 0.75). Thus, this study suggested the potential of vB_SalS_KFSSM to combat indigenous AR Salmonella.

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Acknowledgements

This work was supported by Korea Institute of Planning and Evaluation for Technology in Food, Agriculture and Forestry (IPET) through High Value-added Food Technology Development Program, funded by Ministry of Agriculture, Food and Rural Affairs (MAFRA)(322014052HD030)

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Korea Institute of Planning and Evaluation for Technology in Food, Agriculture and Forestry (IPET), 322014052HD030, Mi-Kyung Park.

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  1. Jaein Choe and Su-Hyeon Kim have contributed equally to this work

Authors and Affiliations

  1. School of Food Science and Biotechnology, and Food and Bio-Industry Research Institute, Kyungpook National University, Daegu, 41566, Republic of Korea

    Jaein Choe, Su-Hyeon Kim, Ji Min Han & Mi-Kyung Park

  2. KookminBio Corporation, Seoul, 02826, Republic of Korea

    Jong-Hoon Kim & Mi-Sun Kwak

  3. Department of Food and Nutrition, Dongduk Women’s University, Seoul, 02748, Republic of Korea

    Do-Won Jeong

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Choe, J., Kim, SH., Han, J.M. et al. Prevalence of Indigenous Antibiotic-Resistant Salmonella Isolates and Their Application to Explore a Lytic Phage vB_SalS_KFSSM with an Intra-Broad Specificity. J Microbiol. 61, 1063–1073 (2023). https://doi.org/10.1007/s12275-023-00098-6

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