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Analysis of phylogenetic markers for classification of a hydrogen peroxide producing Streptococcus oralis isolated from saliva by a newly devised differential medium

  • Microbial Systematics and Evolutionary Microbiology
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Abstract

Hydrogen peroxide (H2O2) is produced by alpha-hemolytic streptococci in aerobic conditions. However, the suitable method for detection of H2O2-producing streptococci in oral microbiota has not been setup. Here we show that o-dianisidine dye and horseradish peroxidase were useful in tryptic soy agar medium to detect and isolate H2O2-producing bacteria with the detection limit of one target colony in > 106 colony-forming units. As a proof, we isolated the strain HP01 (KCTC 21190) from a saliva sample using the medium and analyzed its characteristics. Further tests showed that the strain HP01 belongs to Streptococcus oralis in the Mitis group and characteristically forms short-chain streptococcal cells with a high capacity of acid tolerance and biofilm formation. The genome analysis revealed divergence of the strain HP01 from the type strains of S. oralis. They showed distinctive phylogenetic distances in their ROS-scavenging proteins, including superoxide dismutase SodA, thioredoxin TrxA, thioredoxin reductase TrxB, thioredoxin-like protein YtpP, and glutaredoxin-like protein NrdH, as well as a large number of antimicrobial resistance genes and horizontally transferred genes. The concatenated ROS-scavenging protein sequence can be used to identify and evaluate Streptococcus species and subspecies based on phylogenetic analysis.

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Acknowledgements

This work was supported by a cooperative research program for Agricultural Science & Technology Development, Rural Development Administration (Grant No. PJ01497402 to HGH) and by an internal research project of the Research Institute of Medical Science, Daegu Catholic University School of Medicine (Grant no. 201621 to YHK)

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Authors and Affiliations

  1. Department of Microbiology, Daegu Catholic University School of Medicine, Daegu, 42472, Republic of Korea

    Ha Pham, Thi Dieu Thuy Tran & Yong-Hak Kim

  2. Applied and Environmental Microbiology Laboratory, School of Earth Sciences and Environmental Engineering, Gwangju Institute of Science and Technology, Gwangju, 61005, Republic of Korea

    Youri Yang & Hor-Gil Hur

  3. Bioremediation Team, National Institute of Agricultural Science, Rural Development Administration, Wanju, Jeonju, 55365, Republic of Korea

    Jae-Hyung Ahn

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Ethical Statement This study was reviewed and approved by the ethics committee of the DCU medical centre (IRB protocol number CR-16-027) for the microbiological testing of saliva without disclosure of private information and obtained informed consent from the sample donor.

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Pham, H., Tran, T.D.T., Yang, Y. et al. Analysis of phylogenetic markers for classification of a hydrogen peroxide producing Streptococcus oralis isolated from saliva by a newly devised differential medium. J Microbiol. 60, 795–805 (2022). https://doi.org/10.1007/s12275-022-2261-2

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