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Transcriptomic and proteomic profiling revealed global changes in Streptococcus thermophilus during pH-controlled batch fermentations

  • Microbial Genetics, Genomics and Molecular Biology
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Abstract

Understanding global changes of physiological processes at the molecular level during the growth of Streptococcus thermophilus is essential for the rational design of cultivation media and the optimization of bioprocesses. Transcriptomics and proteomics were combined to investigate the global changes at the transcript and protein level during the growth of S. thermophilus. The expression of 1396 genes (FDR ≤ 0.001) and 876 proteins (P < 0.05) changed significantly over time. The most remarkable growth phase dependent changes occurred in the late-lag phase and were related to heterofermentation, glycolysis, peptidoglycan biosynthesis, conversion between amino acids and stress response. The present results could provide theoretical guidance for high-cell-density culture, help design cultivation media, and help attain a high biomass of S. thermophilus.

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Acknowledgements

This work was supported by grants from the National Natural Science Foundation of China (31771989), Natural Science Foundation of Heilongjiang Province (C2016023), and Academic Backbone Project of Northeast Agricultural University (15XG21).

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Authors and Affiliations

  1. Key Laboratory of Dairy Science, Ministry of Education, College of Food Science, Northeast Agricultural University, Harbin, 150030, Heilongjiang, P. R. China

    Yali Qiao, Cong Leng, Gefei Liu, Yanjiao Zhang, Xuepeng Lv, Hongyu Chen, Jiahui Sun & Zhen Feng

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  1. Yali Qiao
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  2. Cong Leng
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  3. Gefei Liu
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  4. Yanjiao Zhang
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  5. Xuepeng Lv
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  6. Hongyu Chen
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  7. Jiahui Sun
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  8. Zhen Feng
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Correspondence to Zhen Feng.

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The authors declare that they have no conflict of interest.

Supplemental material for this article may be found at http://www.springerlink.com/content/120956.

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Supplementary material, approximately 7.83 MB.

Supplementary data Table S3. List of translation differences in proteins.

Proteins with at least 1.2-fold change (P < 0.05) were considered to be significant differentially expressed proteins.

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Qiao, Y., Leng, C., Liu, G. et al. Transcriptomic and proteomic profiling revealed global changes in Streptococcus thermophilus during pH-controlled batch fermentations. J Microbiol. 57, 769–780 (2019). https://doi.org/10.1007/s12275-019-8604-y

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  • DOI: https://doi.org/10.1007/s12275-019-8604-y

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