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Recent paradigm shift in the assembly of bacterial tripartite efflux pumps and the type I secretion system

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Abstract

Tripartite efflux pumps and the type I secretion system of Gram-negative bacteria are large protein complexes that span the entire cell envelope. These complexes expel antibiotics and other toxic substances or transport protein toxins from bacterial cells. Elucidating the binary and ternary complex structures at an atomic resolution are crucial to understanding the assembly and working mechanism. Recent advances in cryoelectron microscopy along with the construction of chimeric proteins drastically shifted the assembly models. In this review, we describe the current assembly models from a historical perspective and emphasize the common assembly mechanism for the assembly of diverse tripartite pumps and type I secretion systems.

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Authors and Affiliations

  1. Department of Agricultural Biotechnology, Center for Food Safety and Toxicology, Center for Food and Bioconvergence, Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul, 08826, Republic of Korea

    Inseong Jo & Nam-Chul Ha

  2. Unit on Structural and Chemical Biology of Membrane Proteins, Cell Biology and Neurobiology Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD, 20892, USA

    Jin-Sik Kim

  3. Department of Bioengineering, College of Life Science, Dalian Minzu University, Dalian, Liaoning, 116600, P. R. China

    Yongbin Xu

  4. Electron Microscopy Research Center, Korea Basic Science Institute, Cheongju, 28119, Republic of Korea

    Jaekyung Hyun

  5. Department of Life Science, Chung-Ang University, Seoul, 06974, Republic of Korea

    Kangseok Lee

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  1. Inseong Jo
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  2. Jin-Sik Kim
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  3. Yongbin Xu
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  4. Jaekyung Hyun
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  5. Kangseok Lee
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  6. Nam-Chul Ha
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Correspondence to Nam-Chul Ha.

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Jo, I., Kim, JS., Xu, Y. et al. Recent paradigm shift in the assembly of bacterial tripartite efflux pumps and the type I secretion system. J Microbiol. 57, 185–194 (2019). https://doi.org/10.1007/s12275-019-8520-1

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  • DOI: https://doi.org/10.1007/s12275-019-8520-1

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