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Water-based extracts of Zizania latifolia inhibit Staphylococcus aureus infection through the induction of human beta-defensin 2 expression in HaCaT cells

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Abstract

Zizania latifolia is a perennial herb belonging to the family Gramineae that has been used as a health food in Asian countries. In this study, we investigated the antimicrobial effect of Z. latifolia, which increased human beta-defensin 2 (hBD2) expression in HaCaT cells. hBD2 expression was further increased in cells treated with Z. latifolia extracts and subsequently infected with Staphylococcus aureus. Inversely, S. aureus infection decreased after treatment. The induction of hBD2 in HaCaT cells was mediated by the Toll-like receptor 2 (TLR2) signaling pathway, including the activation of extracellular signal-regulated kinase (ERK) and activator protein 1 (AP-1). Further study using siRNA revealed that hBD2 played an important role in the inhibition of S. aureus infection in HaCaT cells. Our data suggest that Z. latifolia extracts can be used as an antimicrobial ingredient for skin treatment formulas.

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Authors and Affiliations

  1. Graduate School of Biotechnology and Institute of Life Science and Resources, Kyung Hee University, Yongin, 17104, Republic of Korea

    Bo Yeon Kang, Hyoik Jeon & Dae Kyun Chung

  2. Skin Biotechnology Center, Kyung Hee University, Yongin, 17104, Republic of Korea

    Seung-Su Lee, Myun-Ho Bang, Hangeun Kim & Dae Kyun Chung

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  1. Bo Yeon Kang
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  2. Seung-Su Lee
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  3. Myun-Ho Bang
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  4. Hyoik Jeon
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  5. Hangeun Kim
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  6. Dae Kyun Chung
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Correspondence to Hangeun Kim or Dae Kyun Chung.

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Kang, B.Y., Lee, SS., Bang, MH. et al. Water-based extracts of Zizania latifolia inhibit Staphylococcus aureus infection through the induction of human beta-defensin 2 expression in HaCaT cells. J Microbiol. 56, 910–916 (2018). https://doi.org/10.1007/s12275-018-8307-9

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  • DOI: https://doi.org/10.1007/s12275-018-8307-9

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