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Spirosoma daeguensis sp. nov., isolated from beach soil

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Abstract

A Gram-stain-negative, non-motile, non-spore-forming, rod-shaped, aerobic bacterium, designated 15J9-6T, was isolated from beach soil on Jeju Island, South Korea. Strain 15J9-6T, grew at 10–30°C (optimum growth at 25°C) and pH 7–8 (optimum growth at pH 7) on R2A, NA, and TSA agar. Phylogenetically, the strain was closely related to members of the genus Spirosoma (92.3–90.1% 16S rRNA gene sequence similarities) and showed highest sequence similarity to Spirosoma panaciterrae DSM 21099T (92.3%). The G+C content of the genomic DNA of strain 15J9-6T was 45.7 mol%. The strain contained phosphatidylethanolamine, two unidentified aminophospholipids, an unidentified phospholipid, and an unidentified lipid as the major polar lipids; menaquinone MK-7 as the predominant respiratory quinone and summed feature 3 (C16:1 ω6c/C16:1 ω7c; 30.1%), C16:1 ω5c (23.1%), iso C15:0 (13.3%), and C16:0 (8.4%) as the major fatty acids which supported the affiliation of strain 15J9-6T to the genus Spirosoma. The results of physiological and biochemical tests allowed genotypic and phenotypic differentiation of strain 15J9-6T from recognized Spirosoma species. On the basis of its phenotypic properties and phylogenetic distinctiveness, strain 15J9-6T represents a novel species of the genus Spirosoma, for which the name Spirosoma daeguensis sp. nov. is proposed. The type strain is 15J9-6T (=KCTC 52036T =JCM 31995T)

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Authors and Affiliations

  1. School of Applied Biosciences, Kyungpook National University, Daegu, 41566, Republic of Korea

    Nabil Elderiny, Leonid N. Ten, Jae-Jin Lee, Seung-Yeol Lee, Sangkyu Park & Hee-Young Jung

  2. School of Food Science and Biotechnology/Food and Bio-Industry Research Institute, Kyungpook National University, Daegu, 41566, Republic of Korea

    Young-Je Cho

  3. Department of Bio and Environmental Technology, Seoul Women’s University, Seoul, 01797, Republic of Korea

    Myung Kyum Kim

  4. Institute of Plant Medicine, Kyungpook National University, Daegu, 41566, Republic of Korea

    Hee-Young Jung

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  1. Nabil Elderiny
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Elderiny, N., Ten, L.N., Lee, JJ. et al. Spirosoma daeguensis sp. nov., isolated from beach soil. J Microbiol. 55, 678–683 (2017). https://doi.org/10.1007/s12275-017-7211-z

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