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Surface display expression of Bacillus licheniformis lipase in Escherichia coli using Lpp’OmpA chimera

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Abstract

The lipase from Bacillus licheniformis ATCC14580 was displayed on the cell surface of Escherichia coli using Lpp’OmpA as the anchoring protein. The expressed Lpp’OmpA-lipase fusion protein has a molecular weight of approximately 35 kDa, which was confirmed by SDS-PAGE and western blot analysis. The Lpp’OmpA-lipase fusion protein was located on the cell surface, as determined by immunofluorescence confocal microscopy and flow cytometry. The enzyme activity of the surface-displayed lipase showed clear halo around the colony. The cell surface-displayed lipase showed the highest activity of 248.12 ± 9.42 U/g (lyophilized cell) at the optimal temperature of 37°C and pH 8.0. The enzyme exhibited the highest activity toward the substrate p-nitrophenyl caprylate (C8). These results suggest that E. coli, which displayed the lipase on its surface, could be used as a whole cell biocatalyst.

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Authors and Affiliations

  1. Department of Bioscience and Biotechnology and Protein Research Center of GRRC, College of Natural Sciences, Hankuk University of Foreign Studies, Kyunggi-Do, 449-791, Republic of Korea

    Jae-Hyung Jo, Chan-Wook Han, Seung-Hwan Kim, Hyuk-Jin Kwon & Hyune-Hwan Lee

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  1. Jae-Hyung Jo
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  2. Chan-Wook Han
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  3. Seung-Hwan Kim
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  4. Hyuk-Jin Kwon
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  5. Hyune-Hwan Lee
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Correspondence to Hyune-Hwan Lee.

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Jo, JH., Han, CW., Kim, SH. et al. Surface display expression of Bacillus licheniformis lipase in Escherichia coli using Lpp’OmpA chimera. J Microbiol. 52, 856–862 (2014). https://doi.org/10.1007/s12275-014-4217-7

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  • DOI: https://doi.org/10.1007/s12275-014-4217-7

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