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Metal-organic framework hybrid materials of ZIF-8/RGO for immobilization of D-amino acid dehydrogenase

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Abstract

Immobilization of D-amino acid dehydrogenase (DAADH) by the assembly of peptide linker was studied for the biosynthesis of D-phenylalanine. Hybrid material of zeolitic imidazolate framework-8 (ZIF-8) combined with reduced graphene oxide (RGO) was applied for the immobilization of DAADH from Ureibacillus thermosphaericus. The recovery rate of DAADH/ZIF-8/RGO was 165.6%. DAADH/ZIF-8/RGO remained 53.4% of its initial activity at 50 °C for 10 h while the free enzyme was inactivated. DAADH/ZIF-8/RGO maintained 70.5% activity in hyperalkaline solution with pH 12. Kinetic parameters indicated that DAADH/ZIF-8/RGO had greater affinity of phenylpyruvate as Vmax/Km of DAADH/ZIF-8/RGO was 1.27-fold than free enzyme. After seven recycles, the activity of DAADH/ZIF-8/RGO remained 64.3%. Furthermore, one-step separation and in situ immobilization of DAADH by ZIF-8/RGO/Ni was carried out with 1.5-fold activity enhancement. Combining peptide linker and metal-organic framework (MOF) immobilization, thermostability and activity of the immobilized DAADH were significantly improved.

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (Nos. 21776233 and 22078273) and National Key Research and Development Program (No. 2022YFA0912000).

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Authors and Affiliations

  1. Department of Chemical and Biochemical Engineering, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, 361005, China

    Hangbin Lei, Qian Zhang, Xiaoyan Xiang, Liang Jiang, Shiyan Wang, Lingxuan Duan & Shizhen Wang

  2. Xiamen Key Laboratory of Synthetic Biotechnology, Xiamen University, Xiamen, 361005, China

    Shizhen Wang

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  1. Hangbin Lei
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  2. Qian Zhang
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  3. Xiaoyan Xiang
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Correspondence to Shizhen Wang.

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Lei, H., Zhang, Q., Xiang, X. et al. Metal-organic framework hybrid materials of ZIF-8/RGO for immobilization of D-amino acid dehydrogenase. Nano Res. 17, 290–296 (2024). https://doi.org/10.1007/s12274-023-5811-y

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  • DOI: https://doi.org/10.1007/s12274-023-5811-y

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