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Two new benzylisoquinoline alkaloids from Thalictrum foliolosum and their antioxidant and in vitro antiproliferative properties

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Abstract

Two novel rare chloro-containing benzylisoquinoline alkaloids, thalfoliolosumines A (1) and B (2), along with eight known isoquinoline alkaloids (3–10) were isolated from the whole plant of Thalictrum foliolosum. The structures of these compounds were elucidated by spectral analyses, including 1D and 2D NMR (COSY, HSQC, HMBC and NOESY) experiments. The antiproliferative effects of all the isolated compounds were evaluated by MTT method against MCF-7, PC-3, and U937 cells, and trypan blue method against HL-60 cells. New compounds 1 and 2 exhibited moderate in vitro antiproliferative activity against MCF-7, PC-3, and HL-60 cells, and good inhibitory effects against U937 cells with IC50 values of 7.50 and 6.97 μM, respectively. Compounds 7 and 10 showed the strongest in vitro antiproliferative with IC50 values of 0.93 and 1.69 μM against HL-60 cell line. The antioxidant properties were also measured, bisbenzyltetrahydroisoquinoline alkaloids 3–6 showed the strongest antioxidant activities in ABTS assay.

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Acknowledgments

This work was financially supported by the National Natural Science Foundation of China (31570350, 21502121), Project Funded by China Post Doctoral Science Foundation (2015M570258), General Scientific Research Projects of Department of Education in Liaoning Province (L2014382), Young Teachers’ Scientific Research Fund Project of Shenyang Pharmaceutical University (QNJJ 2013501) and Career Development Support Plan for Young and Middle-aged Teachers in Shenyang Pharmaceutical University.

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    Authors and Affiliations

    1. Key Laboratory of Structure-Based Drug Design & Discovery, Ministry of Education, and School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang, 110016, China

      Da-hong Li, Jia Guo, Nan Zhao, Kai-bo Wang, Jian-yong Li, Zhan-lin Li & Hui-ming Hua

    2. State Key Laboratory of New-tech for Chinese Medicine Pharmaceutical Processes, Jiangsu Kanion Pharmaceutical Co. Ltd, Lianyungang, 222001, China

      Da-hong Li

    3. School of Life Science and Biopharmaceutics, Shenyang Pharmaceutical University, Shenyang, 110016, China

      Wen Bin

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    1. Da-hong Li
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    Correspondence to Zhan-lin Li or Hui-ming Hua.

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    Da-Hong Li and Jia Guo have contributed equally to this study.

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    Li, Dh., Guo, J., Bin, W. et al. Two new benzylisoquinoline alkaloids from Thalictrum foliolosum and their antioxidant and in vitro antiproliferative properties. Arch. Pharm. Res. 39, 871–877 (2016). https://doi.org/10.1007/s12272-016-0724-x

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