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Measuring levels of biogenic amines and their metabolites in rat brain tissue using high-performance liquid chromatography with photodiode array detection

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Abstract

We developed a method to detect biogenic amines and their metabolites in rat brain tissue using simultaneous high-performance liquid chromatography and a photodiode array detection. Measurements were made using a Hypersil Gold C-18 column (250 × 2.1 mm, 5 µm). The mobile phase was 5 mM perchloric acid containing 5 % acetonitrile. The correlation coefficient was 0.9995–0.9999. LODs (S/N = 3) and LOQs (S/N = 10) were as follows: dopamine 0.4 and 1.3 pg, 3, 4-dihydroxyphenylacetic acid 8.4 and 28.0 pg, serotonin 0.4 and 1.3 pg, 5-hydroxyindolacetic acid 3.4 and 11.3 pg, and homovanillic acid 8.4 and 28.0 pg. This method does not require derivatization steps, and is more sensitive than the widely used HPLC–UV method.

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Acknowledgments

This study was supported by the Basic Science Research Program through a grant funded by the National Research Foundation of Korea (NRF), which is supported by the Korean government (MEST) (NRF-2012R1A1B3003074).

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    1. Department of Oriental Pharmaceutical Sciences, Kyung Hee East–West Pharmaceutical Research Institute, College of Pharmacy, Kyung Hee University, Hoegi-dong, Dongdaemoon-gu, Seoul, 130-701, South Korea

      Min-Jung Gu, Ji-Hyun Jeon, Myung Sook Oh & Seon-Pyo Hong

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    1. Min-Jung Gu
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    2. Ji-Hyun Jeon
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    Correspondence to Seon-Pyo Hong.

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    Min-Jung Gu and Ji-Hyun Jeon have contributed equally to the work.

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    Gu, MJ., Jeon, JH., Oh, M.S. et al. Measuring levels of biogenic amines and their metabolites in rat brain tissue using high-performance liquid chromatography with photodiode array detection. Arch. Pharm. Res. 39, 59–65 (2016). https://doi.org/10.1007/s12272-015-0661-0

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