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Antityrosinase and antimicrobial activities from Thai medicinal plants

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Abstract

Various dermatological disorders and microbial skin infection can cause hyperpigmentation. Therefore, screenings for whitening and antimicrobial agents from Thai medicinal plants have been of research interest. Seventy-seven ethanol plant extracts were investigated for antityrosinase activity, eleven samples showed the tyrosinase inhibition more than 50 % were further preliminary screening for antimicrobial activity by agar disc diffusion and broth micro-dilution methods. Artocarpus integer (Thunb.) Merr. (Moraceae) root extract, which showed the potential of tyrosinase inhibition with 90.57 ± 2.93 % and antimicrobial activity against Staphylococcus aureus, S. epidermidis, Propionibacterium acnes and Trichophyton mentagophytes with inhibition zone as 9.10 ± 0.00, 10.67 ± 0.09, 15.25 ± 0.05 and 6.60 ± 0.17 mm, respectively was selected for phytochemical investigation. Three pure compounds were isolated as artocarpin, cudraflavone C and artocarpanone. And artocarpanone exhibited anti-tyrosinase effect; artocarpin and cudraflavone C also showed the potential of antibacterial activity against S. aureus, S. epidermidis and P. acnes with MIC at 2, 4 and 2 μg/ml, respectively and MBC at 32 μg/ml for these bacteria. So, these pure compounds are interesting for further study in order to provide possibilities of new whitening and antibacterial development. This will be the first report of phytochemical investigation of A. integer root.

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References

  • Alam, M., H.B. Gladstone, and R.C. Tung (eds.). 2009. Cosmetic dermatology, 17–23. China: Elsevier.

    Google Scholar 

  • Athikomkulchai, S., R. Watthanachaiyingcharoen, S. Tunvichien, P. Vayumhasuwan, P. Karnsomkiet, P. Sae-Jong, and N. Ruangrungsi. 2008. The development of anti-acne products from Eucalyptus globulus and Psidium guajava oil. Journal of Health Research 22(3): 109–113.

    CAS  Google Scholar 

  • Burdock, G.A., M.G. Soni, and I.G. Carabin. 2001. Evaluation of health aspects of kojic acid in food. Regulatory Toxicology and Pharmacology 33(1): 80–101.

    Article  PubMed  CAS  Google Scholar 

  • Chaisawadi, S., Thongbute, D., Methawiriyasilp, W., Chaisawadi, A., Pitakworrarat, N., Jaturronrasamee, K., and Suthipinitthum, A. 2008. Preliminary study of antimicrobial activities on medicinal herbs of Thai food’s ingredients. http://www.scisoc.or.th/stt/28/web/content/J_10/J11.html. Accessed 2 Jul 2008.

  • Chen, J.S., C. Wei, and M.R. Marshall. 1991. Inhibition mechanism of kojic acid on polyphenol oxidase. Journal of Agriculture and Food Chemistry 39(11): 1897–1901.

    Article  CAS  Google Scholar 

  • Clausa, H., and H. Decker. 2006. Bacterial tyrosinases. Systematic and Applied Microbiology 29: 3–14.

    Article  CAS  Google Scholar 

  • Cushnie, T.P.T., and A. Lamb. 2005. Antimicrobial activity of favonoids. International Journal of Antimicrobial Agents 26: 343–356.

    Article  PubMed  CAS  Google Scholar 

  • Dominguez-Soto, L., T. Hojyo-Tomoko, E. Vega-Memije, R. Arenas, and R. Cores-Franco. 1994. Pigmentary problems in the topics. Dermatologic Clinics 12(4): 777–784.

    PubMed  CAS  Google Scholar 

  • García-Molina, F., L.G. Fenoll, J.C. Morote, P.A. García-Ruiz, J.N. Rodríguez-López, F. García-Cánovas, and J. Tudela. 2005. Opposite effects of peroxidase in the initial stages of tyrosinase-catalysed melanin biosynthesis. International Journal of Biochemistry and Cell Biology 37: 1179–1196.

    Article  PubMed  CAS  Google Scholar 

  • Gąsowska, B., B. Frąckowiak, and H. Wojtasek. 2006. Indirect oxidation of amino acid phenylhydrazides by mushroom tyrosinase. Biochimica et Biophysica Acta 1760(9): 1373–1379.

    Article  PubMed  CAS  Google Scholar 

  • Gómez, B.L., and J.D. Nosanchuk. 2002. Melanin and fungi. Current Opinion in Infectious Diseases 16(2): 91–96.

    Article  Google Scholar 

  • Hoogduijn, M.J., E. Cemeli, K. Ross, D. Anderson, A.J. Thody, and J.M. Wooda. 2004. Melanin protects melanocytes and keratinocytes against H2O2-induced DNA strand breaks through its ability to bind Ca2+. Experimental Cell Research 294: 60–67.

    Article  PubMed  CAS  Google Scholar 

  • Kiken, D.A., and D.E. Cohen. 2002. Contact dermatitis to botanical extracts. American Journal of Contact Dermatitis 13: 148–152.

    Article  PubMed  Google Scholar 

  • Kim, Y.J., and H. Uyama. 2005. Tyrosinase inhibitors from natural and synthetic sources: structure, inhibition mechanism and perspective. Cellular and Molecular Life Sciences 65: 1707–1723.

    Article  CAS  Google Scholar 

  • Kondo, S., C. Sattaponpan, S. Phongpaichit, A. Srijan, and A. Itharat. 2010. Antibacterail activity of Thai medicinal plants Pikutbenjakul. Journal of the Medical Association of Thailand 93(12): 131–135.

    Google Scholar 

  • Kongcharoensuntorn, W., Chomosot, N., Jindamol, J., Arrayasillapathon, J., Charadram, P., Ounarom, S. and Pittayaphasertgul, A. 2005. Screening of some Thai medicinal plants for antimicrobial activity and antioxidant activity against microorganisms (proceeding), 31st Congress on Science and Technology of Thailand at Suranaree University of Technology, 18–20 Oct 2005.

  • Kumar, G.S., K.N. Jayaveera, C.K.A. Kumar, U.P. Sanjay, B.M.V. Swamy, and D.V.K. Kumar. 2007. Antimicrobial effects of Indian medicinal plants against acne-inducing bacteria. Pharmaceutical Research 6(2): 717–723.

    Google Scholar 

  • Kummee, S., and N. Intaraksa. 2008. Antimicrobial activity of Desmos chinensis leaf and Maclura cochinchinensis wood extract. Songklanakarin Journal of Science and Technology 30(5): 635–639.

    Google Scholar 

  • Lorian, V. 2005. Antibiotics in laboratory medicine, 5th ed. Philadelphia: Lippincott Williams & Wilkins.

    Google Scholar 

  • Lui, G.Y., and V. Nizet. 2009. Color me bad: microbial pigments as virulence factors. Trends in Microbiology 660: 1–8.

    Google Scholar 

  • Niyomkam, P. (2007). Screening of antibacterial activity of Thai medicinal plants against Propionibacterium acnes and preliminary formulation study. A thesis in partial fulfillment of the requirements for the Degree of Master of Pharmacy in Pharmaceutical Sciences, Prince of Songkla University, Songkhla.

  • Noh, J.-M., S.-Y. Kwak, H.-S. Seo, J.-H. Seo, B.-G. Kim, and Y.-S. Lee. 2009. Kojic acid-amino acid conjugates as tyrosinase inhibitors. Bioorganic & Medicinal Chemistry Letters 19: 5586–5589.

    Article  CAS  Google Scholar 

  • Okunji, C., S. Komarnytsky, G. Fear, A. Poulev, D.M. Ribnicky, P.I. Awachie, Y. Ito, and I. Raskin. 2007. Preparative isolation and identification of tyrosinase inhibitors from the seeds of Garcinia kola by high-speed counter-current chromatography. Journal of Chromatography A 1151: 45–50.

    Article  PubMed  CAS  Google Scholar 

  • Pandya, A.G., and I.L. Guevara. 2000. Disorders of hyperpigmentation. Dermatologic Clinics 18(1): 91–98.

    Article  PubMed  CAS  Google Scholar 

  • Peñalver, M.J., L.G. Fenoll, J.N. Rodríguez-López, P.A. García-Ruiz, F. García-Molina, R. Varón, F. García-Cánovas, and J. Tudela. 2005. Reaction mechanism to explain the high kinetic autoactivation of tyrosinase. Journal of Molecular Catalysis B 33: 35–42.

    Article  CAS  Google Scholar 

  • Petit, L., and G.E. Piérard. 2003. Skin-lightening products revisited. International Journal of Cosmetic Science 25: 169–181.

    Article  PubMed  CAS  Google Scholar 

  • Phongpaichit, S., S. Kummee, L. Nilrat, and A. Itarat. 2006. Antimicrobial activity of Cinnamomum porrectum oil. Songklanakarin Journal of Science and Technology 29(1): 11–16.

    Google Scholar 

  • Schurink, M., W.J.H. van Berkel, H.J. Wichers, and C.G. Boeriu. 2007. Novel peptides with tyrosinase inhibitory activity. Peptides 28: 485–495.

    Article  PubMed  CAS  Google Scholar 

  • Shimizu, K., R. Kondo, K. Sakai, S. Buabarn, and U. Dilokkunanant. 2000. A geranylated chalcone with 5α-reductase inhibitory properties from Artocarpus incisus. Phytochemistry 54: 737–739.

    Article  CAS  Google Scholar 

  • Sritularak, B. 1998. Chemical constituents of Artocarpus lakoocha and A. gomezianus. Master’s thesis, Department of Pharmacognosy, Gradute School, Chulalongkorn University, Bangkok.

  • Sritularak, B., W. De-Eknamkul, and K. Likhitwitayawuid. 1998. Tyrosinase inhibitors from Artocarpus lakoocha. Thai Journal of Pharmaceutical Sciences 22: 149–155.

    Google Scholar 

  • Tengamnuay, P., K. Pengrungruangwong, I. Pheansri, and K. Likhitwitayawuid. 2006. Artocarpus lakoocha heartwood extract as novel cosmetic ingredient: evaluation of the in vitro anti-tyrosinase and in vivo skin whitening activities. International Journal of Cosmetic Science 28: 269–276.

    Article  PubMed  CAS  Google Scholar 

  • Wei, B.L., G.R. Weng, P.H. Chiu, C.F. Hung, J.P. Wang, and C.N. Lin. 2005. Antiinflammatory flavonoids from Artocarpus heterophyllus and Artocarpus communis. Journal of Agricultural and Food Chemistry 53: 3867–3871.

    Article  PubMed  CAS  Google Scholar 

  • Zheng, Z.-P., Q. Zhu, C.-L. Fan, H.-Y. Tan, and M. Wang. 2011. Phenolic tyrosiaanse inhibitors from the stems of Cudrania cochinchinensis. Food and Function 2: 259–264.

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgments

The authors are thankful to Prince of Songkla University for financial support of this research.

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Authors and Affiliations

  1. Department of Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmaceutical Sciences, Prince of Songkla University, Hat-Yai, Songkhla, 90112, Thailand

    Sukanya Dej-adisai, Imron Meechai, Jindaporn Puripattanavong & Sopa Kummee

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  1. Sukanya Dej-adisai
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  2. Imron Meechai
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  3. Jindaporn Puripattanavong
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  4. Sopa Kummee
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Correspondence to Sukanya Dej-adisai.

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Dej-adisai, S., Meechai, I., Puripattanavong, J. et al. Antityrosinase and antimicrobial activities from Thai medicinal plants. Arch. Pharm. Res. 37, 473–483 (2014). https://doi.org/10.1007/s12272-013-0198-z

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  • DOI: https://doi.org/10.1007/s12272-013-0198-z

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