Abstract
We investigated the characteristics of spontaneous contraction in feline ileal circular smooth. Smooth muscle contractions were recorded by an isometric force transducer. The neurotoxin tetrodotoxin did not alter the spontaneous contraction of the circular muscles. Atropine or guanethidine also did not affect on the contraction. In Ca2+-free Krebs, the spontaneous contraction completely disappeared in the muscles. An L-type Ca2+ channel blockade with nimodipine decreased the amplitude of spontaneous contraction. A selective inhibitor of the sarcoplasmic reticulum Ca2+-ATPase, thapsigargin, had not no effect on spontaneous contraction within incubation time for 10min. However, phosphoinositide-specific phopholipase C inhibitor, U-73122, decreased the frequency of the contraction in circular smooth muscle These results suggest that the neuronal component, adrenergic or cholinergic innervation was not involved in spontaneous activity and that extracellular Ca2+ influx via an L-type Ca2+ channel may mediate the contractile amplitude in circular smooth muscles of cat ileum.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Berridge, M. J., Inositol trisphosphate and calcium signalling. Nature, 361, 315–325 (1993).
Boddy, G., Bong, A., Cho, W., and Daniel, E. E., ICC pacing mechanisms in intact mouse intestine differ from those in cultured or dissected intestine. Am. J. Physiol. Gastrointest. Liver. Physiol., 286, G653–662 (2004).
Boddy, G. and Daniel, E. E., Role of l-Ca(2+) channels in intestinal pacing in wild-type and W/W(V) mice. Am. J. Physiol. Gastrointest. Liver. Physiol., 288, G439–446 (2005).
Dahms, V., Prosser, C. L., and Suzuki, N., Two types of ’slow waves’ in intestinal smooth muscle of cat. J. Physiol., 392, 51–69 (1987).
Hara, Y., Kubota, M., and Szurszewski, J. H., Electrophysiology of smooth muscle of the small intestine of some mammals. J. Physiol., 372, 501–520 (1986).
Horowitz, B., Ward, S. M., and Sanders, K. M., Cellular and molecular basis for electrical rhythmicity in gastrointestinal muscles. Annu. Rev. Physiol., 61, 19–43 (1999).
Kim, Y. C., Koh, S. D., and Sanders, K. M., Voltage-dependent inward currents of interstitial cells of Cajal from murine colon and small intestine. J. Physiol., 541, 797–810 (2002).
Lee, H. T., Hennig, G. W., Fleming, N. W., Keef, K. D., Spencer, N. J., Ward, S. M., et al.. The mechanism and spread of pacemaker activity through myenteric interstitial cells of Cajal in human small intestine. Gastroenterology, 132, 1852–1865 (2007).
Malysz, J., Donnelly, G., and Huizinga, J. D., Regulation of slow wave frequency by IP(3)-sensitive calcium release in the murine small intestine. Am. J. Physiol. Gastrointest. Liver. Physiol., 280, G439–448 (2001).
Malysz, J., Richardson, D., Farraway, L., Christen, M. O., and Huizinga, J. D., Generation of slow wave type action potentials in the mouse small intestine involves a non-Ltype calcium channel. Can. J. Physiol. Pharmacol., 73, 1502–1511 (1995).
Ozaki, H., Stevens, R. J., Blondfield, D. P., Publicover, N. G., and Sanders, K. M., Simultaneous measurement of membrane potential, cytosolic Ca2+, and tension in intact smooth muscles. Am. J. Physiol., 260, C917–925 (1991).
Prosser, C. L., Rhythmic potentials in intestinal muscle. Fed. Proc., 37, 2153–2157 (1978).
Putney, J. W., Jr., Takemura, H., Hughes, A. R., Horstman, D. A., and Thastrup, O., How do inositol phosphates regulate calcium signaling? FASEB J., 3, 1899–1905 (1989).
Sanders, K. M., A case for interstitial cells of Cajal as pacemakers and mediators of neurotransmission in the gastrointestinal tract. Gastroenterology, 111, 492–515 (1996).
Sanders, K. M., Koh, S. D., Ordog, T., and Ward, S. M., Ionic conductances involved in generation and propagation of electrical slow waves in phasic gastrointestinal muscles. Neurogastroenterol. Motil., 16, 100–105 (2004).
Sanders, K. M., Koh, S. D., and Ward, S. M., Interstitial cells of cajal as pacemakers in the gastrointestinal tract. Annu. Rev. Physiol., 68, 307–343 (2006).
Sanders, K. M., Ordog, T., Koh, S. D., Torihashi, S., and Ward, S. M., Development and plasticity of interstitial cells of Cajal. Neurogastroenterol. Motil., 11, 311–338 (1999).
Taylor, A. B., Kreulen, D., and Prosser, C. L., Electron microscopy of the connective tissues between longitudinal and circular muscle of small intestine of cat. Am. J. Anat., 150, 427–441 (1977).
Thastrup, O., Role of Ca2(+)-ATPases in regulation of cellular Ca2+ signalling, as studied with the selective microsomal Ca2(+)-ATPase inhibitor, thapsigargin. Agents Actions, 29, 8–15 (1990).
Thomsen, L., Robinson, T. L., Lee, J. C., Farraway, L. A., Hughes, M. J., Andrews, D. W., et al., Interstitial cells of Cajal generate a rhythmic pacemaker current. Nat. Med., 4, 848–851(1998).
Torihashi, S., Fujimoto, T., Trost, C., and Nakayama, S., Calcium oscillation linked to pacemaking of interstitial cells of Cajal: requirement of calcium influx and localization of TRP4 in caveolae. J. Biol. Chem., 277, 19191–19197 (2002).
Ward, S. M., Ordog, T., Koh, S. D., Baker, S. A., Jun, J. Y., Amberg, G. et al., Pacemaking in interstitial cells of Cajal depends upon calcium handling by endoplasmic reticulum and mitochondria. J. Physiol., 525, 355–361 (2000).
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Park, S.Y., Je, H.D., Shim, J.H. et al. Characteristics of spontaneous contraction in the circular smooth muscles of cat ileum. Arch. Pharm. Res. 33, 159–165 (2010). https://doi.org/10.1007/s12272-010-2238-2
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12272-010-2238-2