Abstract
Ecklonia stolonifera is a rich source of phlorotannins, which are responsible for the potent pharmacological effects associated with this seaweed. The purpose of this study was to develop a reversed-phase high-performance liquid chromatography method for the simultaneous determination of three major phlorotannins, eckol, dieckol, and phlorofucofuroeckol-A, in the extracts of Ecklonia stolonifera. The optimal chromatographic conditions were achieved on a Thermo Hypersil Gold C-18 column (250 × 4.6 mm i.d., 5 μm) using linear gradient elution of acetonitrile and water containing 0.1% formic acid at UV 254 nm. The separated phlorotannins were identified by liquid chromatography-mass spectrometry. The high-performance liquid chromatography method showed good linearity (r2 > 0.998), precision (1.4–9.5%), and accuracy (93.9–108.7%). The limits of detection ranged from 0.06 to 0.30 μg/mL and the lower limits of quantitation ranged from 0.2 to 1.0 μg/mL. Among phlorotannins, dieckol was the most abundant in both ethanol and ethyl acetate extracts of Ecklonia stolonifera.
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References
Glombitza, K. W., Hauperich, S., and Keusgen, M., Phlorotannins from the brown algae Cystophora torulosa and Sargassum spinuligerum. Nat. Toxins, 5, 58–63 (1997).
Goo, H. R., Choi, J. S., and Na, D. H., Simultaneous determination of quercetin and its glycosides from the leaves of Nelumbo nucifera by reversed-phase high-performance liquid chromatography. Arch. Pharm. Res., 32, 201–206 (2009).
Iwai, K., Antidiabetic and antioxidant effects of polyphenols in brown alga Ecklonia stolonifera in genetically diabetic KK-Ay mice. Plant Foods Hum. Nutr., 63, 163–169 (2008).
Joe, M. J., Kim, S. N., Choi, H. Y., Shin, W. S., Park, G. M., Kang, D. W., and Kim, Y. K., The inhibitory effects of eckol and dieckol from Ecklonia stolonifera on the expression of matrix metalloproteinase-1 in human dermal fibroblasts. Biol. Pharm. Bull., 29, 1735–1739 (2006).
Jung, H. A., Hyun, S. K., Kim, H. R., and Choi, J. S., Angiotensin-converting enzyme I inhibitory activity of phlorotannins from Ecklonia stolonifera. Fish. Sci., 72, 1292–1299 (2006).
Kang, H. S., Chung, H. Y., Jung, J. H., Son, B. W., and Choi, J. S., A New phlorotannin from the Brown Alga Ecklonia stolonifera. Chem. Pharm. Bull., 51, 1012–1014 (2003).
Kang, H. S., Chung, H. Y., Kim, J. Y., Son, B. W., Jung, H. A., and Choi, J. S., Inhibitory Phlorotannins from the edible brown alga Ecklonia stolonifera on total reactive oxygen species (ROS) generation. Arch. Pharm. Res., 27, 194–198 (2004).
Kim, A. R., Shin, T. S., Lee, M. S., Park, J. Y., Park, K. E., Yoon, N. Y., Kim, J. S., Choi, J. S., Jang, B. C., Byun, D. S., Park, N. K., and Kim, H. R., Isolation and identification of phlorotannins from Ecklonia stolonifera with antioxidant and anti-inflammatory properties. J. Agric. Food Chem., 57, 3483–3489 (2009).
Kovikko, R., Loponen, J., Pihlaja, K., and Jormalainen, V., High-performance liquid chromatographic analysis of phlorotannins from the brown alga Fucus Vesiculosus. Phytochem. Anal., 18, 326–332 (2007).
Koivikko, R., Eranen, J. K., Loponen, J., and Jormalainen, V., Variation of phlorotannins among three populations of Fucus vesiculosus as revealed by HPLC and colorimetric quantification. J. Chem. Ecol., 34, 57–64 (2008).
Nakamura, T., Nagayama, K., Uchida, K., and Tanaka, R., Antioxidant activity of phlorotannins isolated from the brown alga Eisenia bicyclis. Fish. Sci., 62, 923–926 (1996).
Ragan, M. A. and Glombitza, K. W., Phlorotannins, brown algal polyphenols. In Round, F. E. and Chapman, D. J. (Eds.). Progress in Phycological Research. Biopress, Bristol, pp. 129–241, (1986).
Sailler, B. and Glombitza, K. W., Halogenated phlortethols and fucophlorethols from the brown alga Cytophora retroflexa. Nat. Toxins, 7, 57–62 (1999).
Steinhoff, F. S., Wiencke, C., Müller, R., and Bischof, K., Effects of ultraviolet radiation and temperature on the ultrastructure of zoospores of the brown macroalga Laminaria hyperborea. Plant Biol., 10, 388–397 (2008).
Stern, J. L., Hagerman, A. E., Steinberg, P. D., Winter, F. C., and Estes, J. A., A new assay for quantifiying brown algal phlorotannins and comparisons to previous methods. J. Chem. Ecol., 22, 1273–1293 (1996).
Yoon, N. Y., Chung, H. Y., Kim, H. R., and Choi, J.S., Acetyland butyrlcholinesterase inhibitory activities of sterols and phlorotannins from Ecklonia stolonifera. Fish. Sci., 74, 200–207 (2008a).
Yoon, N. Y., Kim, H. R., Chung, H. Y., and Choi, J. S., Antihyperlipidemic effect of an edible brown algae, Ecklonia stolonifera, and its constituents on poloxamer 407-induced hyperlipidemic and cholesterol-fed rats. Arch. Pharm. Res., 31, 1564–1571 (2008b).
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Goo, H.R., Choi, J.S. & Na, D.H. Quantitative determination of major phlorotannins in Ecklonia stolonifera . Arch. Pharm. Res. 33, 539–544 (2010). https://doi.org/10.1007/s12272-010-0407-y
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DOI: https://doi.org/10.1007/s12272-010-0407-y