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The effect of lead on calcium release activated calcium influx in primary cultures of human osteoblast-like cells

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Abstract

To further understand the significance of bone as a target tissues of lead toxicity, as well as a reservoir of systemic lead, it is necessary to define the effect of lead on the calcium release activated calcium influx (CRACI) in primary cultures of human osteoblast-like cells (OLC). Pb2+ inhibited the immediate CRACI dose-dependent manner. Influx of Pb2+ into human OLC was increased dose-dependent manner. The present study demonstrates that the interference of Pb2+ with CRACI of human OLC is at least twofold: (1) the initiation of CRACI, i.e., the measurable influx of Ca2+ upon Ca2+ readdition, is partially inhibited by Pb2+ and (2) the influx of Pb2+ was enhanced after CRACI had been induced.

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Authors and Affiliations

  1. Departments of Dental Pharmacology and Biophysics, College of Dentistry and Research Institute for Oral Biotechnology Pusan National University, Busan, 602-739, Korea

    Hye-Ock Jang, Jeong-Kuk Kim, Myung-Suk Ko, Dong-Hoo Kim, Won-Il Kim & Il Yun

  2. Department of Microbiology, College of Dentistry and Research Institute for Oral Biotechnology Pusan National University, Busan, 602-739, Korea

    Ji-Suk Kim & Jin Chung

  3. Departments of Oral and Maxillofacial Surgery and Clinical Pharmacology, College of Dentistry and Research Institute for Oral Biotechnology Pusan National University, Busan, 602-739, Korea

    Woo-Cheol Kwon, In-Kyo Chung & Sang-Hun Shin

  4. Department of Prosthodontics, College of Dentistry and Research Institute for Oral Biotechnology Pusan National University, Busan, 602-739, Korea

    Young-Chan Jeon

  5. Departments of Oral Physiology and Molecular Biology, College of Dentistry and Research Institute for Oral Biotechnology Pusan National University, Busan, 602-739, Korea

    Moon-Kyung Bae

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  1. Hye-Ock Jang
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  2. Ji-Suk Kim
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  13. Il Yun
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Correspondence to Il Yun.

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Jang, HO., Kim, JS., Kwon, WC. et al. The effect of lead on calcium release activated calcium influx in primary cultures of human osteoblast-like cells. Arch. Pharm. Res. 31, 188–194 (2008). https://doi.org/10.1007/s12272-001-1140-3

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  • DOI: https://doi.org/10.1007/s12272-001-1140-3

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