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Membranproteinfaltung — Kernwissen aus (nicht zu) vereinfachten Systemen

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  • Membranbiologie
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Abstract

Despite their functional and structural diversity, membrane proteins of all living cells are inserted and assembled via a few evolutionary conserved pathways. Here, we describe how studies in model membranes provide insights into the functionality of the Sec translocon, a primary machinery for the insertion of a-helical membrane proteins, but also for translocation of unfolded polypeptide chains across the membrane. To bring together in vitro and in vivo analysis, further developments of new membrane mimetics are suggested.

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Literatur

  1. Hanczyc MM, Monnard PA (2017) Primordial membranes: more than simple container boundaries. Curr Opin Chem Biol 40:78–86

    Article  CAS  PubMed  Google Scholar 

  2. White H, Wimley C (1999) Membrane protein folding and stability: physical Principles. Annu Rev Biophys Biomol Struct 28:319–365

    Article  CAS  PubMed  Google Scholar 

  3. Park E, Rapoport TA (2012) Mechanisms of Sec61/SecY-mediated protein translocation across membranes. Annu Rev Biophys 41:21–40

    Article  CAS  PubMed  Google Scholar 

  4. Tanaka Y, Sugano Y, Takemoto M et al. (2015) Crystal structures of SecYEG in lipidic cubic phase elucidate a precise resting and a peptide-bound state. Cell Rep 13:1561–1568

    Article  CAS  PubMed  Google Scholar 

  5. Kedrov A, Kusters I, Krasnikov VV et al. (2011) A single copy of SecYEG is sufficient for preprotein translocation. EMBO J 30:4387–4397

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  6. Taufik I, Kedrov A, Exterkate M et al. (2013) Monitoring the activity of single translocons. J Mol Biol 425:4145–4153

    Article  CAS  PubMed  Google Scholar 

  7. Wu ZC, De Keyzer J, Kedrov A et al. (2012) Competitive binding of the SecA ATPase and ribosomes to the SecYEG translocon. J Biol Chem 287:7885–7895

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  8. Kedrov A, Sustarsic M, De Keyzer J et al. (2013) Elucidating the native architecture of the YidC:ribosome complex. J Mol Biol 425:4112–4124

    Article  CAS  PubMed  Google Scholar 

  9. Kedrov A, Wickles S, Crevenna AH et al. (2016) Structural dynamics of the YidC:ribosome complex during membrane protein biogenesis. Cell Rep 17:2943–2954

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  10. Engelman DM (2005) Membranes are more mosaic than fluid. Nature 438:578–580

    Article  CAS  PubMed  Google Scholar 

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Authors and Affiliations

  1. Synthetische Membransysteme, Institut für Biochemie, Universität Düsseldorf, Düsseldorf, Deutschland

    Maryna Löwe, Athanasios Papadopoulos, Michael Kamel & Alexej Kedrov

Authors
  1. Maryna Löwe
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  2. Athanasios Papadopoulos
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  3. Michael Kamel
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  4. Alexej Kedrov
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Corresponding author

Correspondence to Alexej Kedrov.

Additional information

Maryna Löwe Jahrgang 1992. 2015–2017 Masterstudium Biotechnologie mit dem Schwerpunkt medizinische Biotechnologie an der Universität für Bodenkultur in Wien, Österreich. Seit 2018 Promotion an der Universität Düsseldorf.

Athanasios Papadopoulos Jahrgang 1989. 2015–2018 Masterstudium Biologie am Institut für Botanik, Universität Düsseldorf, mit dem Schwerpunkt Astrobiologie und Weltraummikrobiologie in Kollaboration mit dem Deutschen Institut für Luft- und Raumfahrt (DLR). Seit 2018 Promotion an der Universität Düsseldorf.

Michael Kamel Jahrgang 1991. 2015–2017 Masterstudium „Molecular Bioengineering‟ mit Schwerpunkt bioaktive und responsive Polymerforschung an der TU Dresden. Seit 2018 Promotion an der Universität Düsseldorf.

Alexej Kedrov Jahrgang 1979. 2006 Promotion an der TU Dresden und am Max-Planck-Institut für molekulare Zellbiologie und Genetik (MPI-CBG). 2008–2012 Postdoc an der Universität Groningen, Niederlande. 2013–2017 Postdoc und Projektleiter an der LMU München. Seit 2017 Juniorprofessor für Synthetische Membransysteme an der Universität Düsseldorf.

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Löwe, M., Papadopoulos, A., Kamel, M. et al. Membranproteinfaltung — Kernwissen aus (nicht zu) vereinfachten Systemen. Biospektrum 25, 385–387 (2019). https://doi.org/10.1007/s12268-019-1066-2

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  • DOI: https://doi.org/10.1007/s12268-019-1066-2

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